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A. B. mPPAR a. 18. hPPAR a. **. 20. **. **. **. **. **. 12. Relative reporter activity. 15. Relative reporter activity. 10. *. 6. *. 5. *. 0. (-). WY. Rosi. GW. CG269. 0. 1 m M 1 m M 1 m M. (-). WY. Rosi. GW. CG269. 1 m M 1 m M 1 m M. C. D. #. PPAR d. - PowerPoint PPT Presentation
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Appendix 1 Jeong et al.
A.mPPAR
0
6
12
18
(-) WY Rosi GW CG2691M 1M 1M
Rel
ativ
e
rep
ort
er a
ctiv
ity
*
**
****
B.
hPPAR
0
5
10
15
20
(-) WY Rosi GW CG2691M 1M 1M
Rel
ativ
e
rep
ort
er a
ctiv
ity
** ****
**
0
2
4
6
8
(-) WY Rosi GW CG269
Rel
ativ
e
re
po
rter
act
ivit
y
PPARC.
#D.
LXR
Rel
ativ
e
re
po
rter
act
ivit
y
0
2
4
6
8
10
(-) T1317 WY Rosi GW CG269
#
E.
Rel
ativ
e re
po
rter
act
ivit
y EC 50 = 39 nMPPAR
0 0.001 0.01 0.1 1 10(M)
0
5
10
15
//
//F.
PPAR
EC 50 =2.69 M
Rel
ativ
e re
po
rter
act
ivit
y
(M)0 0.001 0.01 0.1 1 10
0
5
10
15
//
////
Appendix 2 Jeong et al.
N
O
F3C
O
N
S
O
HO
OH
OO
O
S
N
Cl
A.
B.
Jeong et al.Appendix 3
*
*
**
B.PPAR
WYGWRosi
Trypsin+
++
++ + + + + +
CG269
+ + + + +
*
*
A.
**
Trypsin
PPARWYGWRosi
++
++
+ + + + + +
CG269
+ + + + +
Jeong et al.Appendix 4
Fo
ld o
f -
oxi
dat
ion
0
0.5
1.0
1.5
(-) WY Rosi CG269
* *
0
2
4
6
8
ACO CPT1Rel
ativ
e g
ene
exp
ress
ion
#
*
#
*
**
**
*
A.
B.
Jeong et al.Appdneix 5
A.
0
2
4
6
8
aP2 Acrp30 LPL CD36Rel
ativ
e g
ene
exp
ress
ion
3T3-L1
#
*
*
*
*
**
*
*#
#
# #*
*
B.
0
2
4
6
8
aP2 Acrp30 LPL CD36
3T3-F442A
Rel
ativ
e g
ene
exp
ress
ion
#
#
**
#
#
**
**
###
* *
Rel
ativ
e m
RN
A PPAR
(-) RosiCG269 (-) RosiCG269 (-) RosiCG2690.0
0.5
1.0
1.5
**
#
*
**
* *
*
*# *# **¶
¶ ¶ ¶
¶
¶
aP2 CD36Acrp30
D.
C.
0
1
2
3
4
aP2 Acrp30 LPL
Rel
ativ
e g
ene
exp
ress
ion
WAT
#*
#
##
*
Veh WYRosi CG269
Jeong et al.Appendix 6
A.
25%
3%
5%
12%9%
4%
42%
Metabolism
oxidation/reduction
Inflammation/immune response
Signaling transduction
Cell growth/division/ apoptosis
Transcription
Others
B.
118
6
15 39
1
Total 246
Rosi
218
CG269
170
85 78
Upregulation Downregulation
100
91 79
C.
CxCl12
Retn
PmvKMthfd1l
Iars
Agpat3
Cfd
Hadhb
Ifi30
Veh CG269 CG269 Rosi 0.5M 5M 1M
1 2 3 4 5 6 7 8
0
2
4
6
8
10
TNF IL-1 IL-6 iNOS MCP1 COX2 MMP9
Rel
ativ
e g
ene
exp
ress
ion
3T3-F442A
*
**
*
*
***
#
#
#
#
#¶¶ ¶
¶ ¶# ¶¶¶ ¶¶¶ ¶
#¶ ¶¶¶¶ ¶ ¶
Jeong et al.Appendix 7
Jeong et al.
Ave
rag
ed
aily
fo
od
inta
ke (
g)
0.0
2.5
5.0
7.5
Veh WY Rosi CG269
Appendix 8
Jeong et al.Appendix 9
Rel
ativ
eg
ene
exp
ress
ion
** *
#
**
*
Jeong et al.Appendix 10
Veh WY Rosi CG269
