D I A B E T E S R E S E A R C H A N D C L I N I C A L P R A C T I C E 1 0 3 S ( 2 0 1 4 ) S 1 – S 6 1 S19

une fraction importante et croissante des personnes âgées et desdiabétiques. La principale cause de décès chez les diabétiques estreprésentée par les maladies cardiovasculaires. Le but de notretravail était d’illustrer les aspects cliniques des complicationsdégénératives du diabétique âgé.

Méthodes : Il s’agissait d’une étude transversale rétrospectivedescriptive qui s’est déroulée sur une période de 2 mois (févrierà mars 2013). Elle a porté sur les diabétiques âgés de 60 ans etplus, suivis au Centre Antidiabétique d’Abidjan.

Résultats : Au total, 157 patients ont été inclus dans l’étude.L’âge moyen était de 66,8±7,2 ans avec des extrêmes de 60 et 91ans. On notait une prédominance féminine dans 55 % des cas. Enmoyenne, la durée d’évolution du diabète était estimée à 9,6±7,9ans. La prévalence de l’hypertension artérielle et des compli-cations dégénératives du diabète était respectivement de 69 %et 95,5 %. La microangiopathie présente chez 139 patients soit92,7 % des cas et était dominée par la polyneuropathie (73,4 %)suivi de la dysfonction érectile (24,5 %). La rétinopathie et la né-phropathie diabétique étaient notées respectivement dans 20,1 %et 15,1 % des cas. Les complications macrovasculaires étaientprésentes chez 19,3 % des patients et a intéressé les trois prin-cipaux lits vasculaires avec comme expression clinique les acci-dents vasculaires cérébraux (41,4 %), les coronaropathies (37,9 %)et l’artériopathie oblitérante des membres inférieurs (34,5 %).

Conclusion : La prévention des complications dégénérativesnotamment des macroangiopathies chez le sujet âgé diabétiquereste une priorité.

OP36Evaluation of nutritional effect of quail eggs on bloodglucose, lipid profile and oxidative stress parameters indiabetic induced rats

E. Lontchi Yimagou a, A. Tanya b, C. Tchankou a, J. Ngondi a,J. Oben a. aLaboratory of Nutrition and NutritionnalBiochemistry/University of Yaounde I; bFaculty of Medicine andBiomedical Sciences, Department of Public Health, University ofYaounde I, Yaounde, Cameroon

Introduction: Quail eggs are a new food item that has recentlybeen introduced into the diet of some Cameroonians. Thesesmall colourful eggs are being advertised in some shops in thecities with many unfounded health claims. One of these claimsis that quail eggs can reduce blood glucose levels in diabetics.It was therefore necessary to evaluate the effect of quail eggson blood glucose, lipid profile and oxidative stress parameters indiabetic induced rats.

Methods: Twenty Wistar rats weighing on average 250 gwere divided into four groups of five each. Group 1 consistedof rats with normal blood glucose and the other 3 groups(2, 3 and 4) consisted of diabetic induced rats by intravenousinjection of streptozotocin. For 16 days, groups 1 and 2 receiveddistilled water, groups 3 and 4 received quail and chicken eggsrespectively with gastro oesophageal probe at a dose of 1 mL/200g body weight. The experiment lasted 17 days. Fasting bloodglucose levels were determined in all groups on the 1st, 7th, 14thand 17th days after induction of diabetes. On the 17th day, thefasting rats were sacrificed; blood and liver were collected forbiochemical analysis.

Results: In 17 days, the consumption of quail and chickeneggs had no effect on blood glucose levels of diabetic rats. Totalcholesterol level were higher in groups 3 (75.59 mg/dL) and4 (59.41 mg/dL) compared to group 2 (55.67 mg/dL) althoughthis difference was non significant. Also triglyceride levels weresignificantly higher (P<0.05) in group 3 (106.52 mg/dL) and 4(109.65 mg/dL) compared to group 2 (65.82 mg/dL). Quail eggshad no effect on oxidative stress parameters (Malondialdehyde,hydroperoxides and catalase).

Conclusion: The consumption of quail eggs by diabetic

patients would have no effect on blood glucose, oxidative stressparameters and may have a negative effect on lipid profile.

OP37Guinean antidiabetic plants and their potential anticanceractivity

E.S. Baldé a, N.M. Baldé b, A. Diallo a, M.S. Traoré a, V. Mégalizzi c,A.M. Baldé a. aCentre de Recherche et de Valorisation des PlantesMédicinales, Dubréka, Guinée, Département de Pharmacie, Faculté deMédecine-Pharmacie-Odontostomatologie, Université de Conakry,Guinée; bService d’endocrinologie, CHU Donka Conakry Guinée,Département de Médecine, FMPOS Université Gamal Abdel Nasser,Conakry, Guinée; cLaboratoire de toxicologie, Institut de Pharmacie,ULB Bruxelles Belgique

Introduction: Previously studies have reported the link betweendiabetes and various cancers. Since plant species have beendescribed to play an important role in diabetes management.The aim of this study was to explore the anticancer activity ofGuinean medicinal plants widely used in the management ofdiabetes.

Methods: Five plants (Anacardium occidentale, Alchornea cordi-folia, Bridellia ferruginea, Cassia siberiana, Piliostigma thonningii)widely used in the Guinean traditional medicine were selectedand two extracts (polar and apolar) were prepared for eachplant. The in vitro antigrowth activity using the colorimetric3-[4,5-dimethylthiazol-2yl]-diphenyl tetrazolium bromide assay(MTT) were evaluated for 4 cancer cells lines (Hs683, U373, MCF7,B16F10).

Results: All the apolar extract displayed a significant growthinhibitory activity against the 4 cancer cell lines studied. Fromwhile the polar extracts only that of Bridellia ferruginea dis-played an activity which was strong. The cytotoxicity wasmoderate (10 μg/ml < IC50 < 50 μg/ml) for Anacardium occiden-tale, Alchornea cordifolia, Cassia siberiana good (1 μg/ml < IC50 <

5 μg/ml) for Piliostigma thonningii and strong (0,1 μg/ml < IC50 <

0,5 μg/ml) for Bridellia ferruginea.Conclusion: These plants may be playing an important role in

cancer chemoprevention. Previous studies reported antioxidantactivity for Piliostigma thonningii and the inhibition of the NF-κBand MAPK, two signaling pathway implicated in the cancer cellproliferation and migration by Bridellia ferruginea, Anacardiumoccidentale. The bio-guided fractionation will be plan for theresearch of the compound with a potential anticancer activity.

OP39Evaluation of the hypoglycemic, hypolipidemic and antialpha amylase effects of extracts of the twigs and fruits ofFicus ovata Vahl (Moraceae)

M. Fouondo, J. Oben, C. Fokunang. University of Yaoundé I

Introduction: The phytochemical component of many medicinalplants have most often been linked to the modulation ofbiomarkers associated to type 2 diabetes. Previous studies donethe hydroethanolic fruits and hydroethanolic twigs of Ficus ovata,revealed the presence of groups of bioactive compounds suchas alkaloids, glycosides, saponins and polyphenols (flavanoids,tannins and phenols). These extracts also showed antioxidantscavenging effects which is an important component in themanagement of type 2 diabetes. This study was aimed atevaluating the in vitro anti alpha amylase, antihyperglycemic andantihyperlipidemic effects of Ficus ovata.

Methods: The hydroethanolic extracts of the fruits andtwigs were used for the in vitro anti alpha amylase assay, apostprandial blood glucose test (OGTT) on normal rats. Moreover,the preventive effects of the extracts against some biomarkers ofdiabetes (lipid profile, endothelia dysfunction, hepatic and renal