Section of Microbiology- Microb Drug Resist. 2016 Jul;22(5):360-3. Growthandadhesionto HT-29 cells inhibition of Gram-negatives by Bifidobacterium longum BB536 e Lactobacillus rhamnosus

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Pio Maria FURNERIAssociate Professor

Adriana GAROZZOAssociate Professor

Viviana CAFISOAssistant Professor

Floriana CAMPANILEAssistant Professor

Maria L. MEZZATESTAAssistant ProfessorSection Coordinator

Ildebrando M. C. PATAMIAAssistant Professor

Raffaela RUSSOAssistant Professor

Mario SALMERIAssistant Professor

Maria SANTAGATIAssistant Professor

Aldo STIVALAAssistant Professor

Laura TROVATOAssistant Professor

Daria NICOLOSIAssistant Professor

Giovanna BLANDINOFull Professor

Stefania STEFANIFull Professor

Guido SCALIAFull Professor

Maria A. TOSCANOFull Professor

Faculty members

Biometec: a research for life

Section of Microbiology

36

• In vitro susceptibility studies to determine the activity of investigational drugs

• Study on antimicrobial resistance mechanisms in MDR Gram- positive and negative microorganisms.

• Characterization and sequencing of genetic elements in Streptococcus spp, Enterococcus spp, Staphylococcus spp, Corynebacterium spp, Enterobacteriaceae and Acinetobacter baumannii.

• Probiotics and Microbiome: screening and use of microorganisms with probiotic characteristics; metagenomic analysis of the oral microbiota using NGS.

• Traditional and molecular diagnostics and Bacterial epidemiology

• Virulence and persistence of Staphylococcus spp , Pseudomonas aeruginosa and Streptococcus pyogenes

• Study of internalization of S.aureus strains, from persistent and recurrent infections in human cell lines, by ex-vivo model and imaging flow-cytometry analysis.

• Helminthiasis among migrants in Sicily: A possible focus for re- emerging neurocysticercosis in Europe

• Symbiotics and aging eye: Evaluation of ocular microbiome.

• Drug delivery system for antimicrobial and antimycotic agents

• Amensalistic behavior of human isolates of lactic bacteria

• Studies on biological biofunctions and role in host interaction and protection against intestinal pathogens of exopolisaccarides produced by Bifidobacterium spp

REASEARCH LINES



• Studies of natural and synthetic antimicrobials

• Molecular mechanisms in bacterial translocation of the blood brain and blood retinal barriers

• Study of the biological activity of natural substances

• Molecular diagnostics and evaluation of humoral and cell- mediated immune response of viral infections of immunocompromised hosts with particular regard to subjects undergoing transplantation of stem cells.

• Study of the role of human papillomavirus (HPV) in infertility

• Diagnosis, prevention and treatment of infections during pregnancy and at-risk newborn.

• Environmental distribution of Cryptococcus neoformans and C. gattii around the Sicily basin

• Susceptibility of Aspergillus terreus species complex infections

• Environmental isolates of multi-azole-resistant Aspergillus spp. in Southern Italy

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38 Biometec: a research for life


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TM Coque, Hospital Universitario Ramón y Cajal, Spain

Science and Engineering Complex University of Tuscaloosa, Alabama, USA

Master in Conversation of Antique Photographs and Paper Heritage, Università Helwan (Il Cairo), Egypt

Water Research and Technologies Centre, Laboratory of Wastewater Treatment. Università di Borj-Cédria (Tunisia).

P Gameiro; Chemistry and Biochemistry Department. University of Porto. Portugal.

M Oggioni, Department Genetics, University of Leicester, Leicester, UK.

AS Bayer Division of Infectious Diseases, Biomedical Research Institute at Harbor-UCLA Medical The David Geffen School of Medicine at UCLA, Los Angeles, California, United States of America

P Ruas-Madiedo, Instituto de Productos Lácteos de Asturias-Consejo Superior de Investigaciones Científicas (IPLA-CSIC) Asturias, Spain.

AW. Friedrich, Department of Medical Microbiology and Infection Control, University of Groningen, Holland

JA Rodríguez, Dpt. de Genètica i Microbiologia, Facultat de Biociències, Universitat Autònoma de Barcelona, BARCELONA (SPAIN)

JB García, Autonomous University of Barcelona, Cerdanyola del Vallès, SpainDepartment Department of Genetics and Microbiology, BARCELONA (SPAIN)

I Toth, The School of Chemistry and Molecular Biosciences (SCMB), The University of Queensland, Brisbane, Queensland 4072, Australia; The School of Pharmacy, The University of Queensland, Brisbane, Queensland 4072, Australia; The Institute for Molecular Biosciences, The University of Queensland, Brisbane, Queensland 4072, Australia.


INTERNATIONAL COLLABORATIONS


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PON 01_02464 PON 2007-2013 - MICRO-MAP; PON 2007-201301_01078

PO-FERS «Diamond HV»

FIR-2014 FIR-2015 FIR-2017

Fellowship Program 2014 (Supporting research To Advance Patient Care)

Nacture srl - spin offUniversity of Catania

Financial support from pharmaceutical companies

2016-2017

PRA-2008

Action1.1.5

1994-2009


www.fir2014.unict.it/UNIVERSITÀ DI CATANIA


AWARDS:Stefania Stefani- Editor in chef of JGAR- Scientific Advisory Committee (SAC) of the European Society of Clinical Microbiology and In fectious diseases (ESCMID)- Member of the sub-Committeefor antimicrobial resistance (EUCAST subcommittee) of the ESCMID.

Research paper selection of Biometec (2014-2018)

Fatal pulmonary disease and encephalic complication in a man with HSV-1 Infection: a case report.Astuto M, Palermo CI, Costanzo CM, Ettorre GC, Palmucci S, Franchina C, Russo R, Valastro P, Timpanaro V, Scalia G. - J. Clin. Virol. 2014; 59, p. 59-62.

dltA overexpression: A strain-independent keystone of daptomycin resistance in methicillin-resistant Staphylococcus aureus.Cafiso V, Bertuccio T, Purrello S, Campanile F, Mammina C, Sartor A, Raglio A, Stefani S. Int J Antimicrob Agents. 2014 Jan;43(1):26-31.

ADV36 adipogenic adenovirus in human liver disease.Trovato FM, Catalano D, Garozzo A, Martines GF, Pirri C, Trovato GM. - World J Gastroenterol. 2014 Oct 28;20(40):14706-16. Review.

Parvovirus B19-associated acute myocarditis in paediatric patients.Palermo CI, Costanzo CM, Trovato L, Franchina C, Russo R, Scalia G. - Infect Dis Trop Med.2015; 1: E193

Insights and clinical perspectives of daptomycin resistance in Staphylococcus aureus: A review of the available evidence.Stefani S, Campanile F, Santagati M, Mezzatesta ML, Cafiso V, Pacini G. - Int J Antimicrob Agents. 2015 Sep;46(3):278-89.

Nanotechnology approaches for antibacterial drug delivery: Preparation and microbiological evaluation of fusogenic liposomes carrying fusidic acid.Nicolosi D, Cupri S, Genovese C, Tempera G, Mattina R, Pignatello R. - Int J Antimicrob Agents. 2015 Jun;45(6):622-6.

Microbiological characteristics of the probiotic strains B. longum BB536 and L. rhamnosus HN001 used in combination.Inturri R, Stivala A, Blandino G. - Minerva Gastroenterol Dietol. 2015 Dec;61(4):191-7.

Focal epilepsy as a long term sequela of Parvovirus B19 encephalitis.Palermo CI, Costanzo CM, Franchina C, Castiglione G, Giuliano L, Russo R, Conti A, Sofia V, Scalia G. - J Clin Virol. 2016; 80, p. 20-23.

Redox modulation of cellular stress response and lipoxin A4 expression by Coriolus versicolor in rat brain: Relevance to Alzheimer’s disease pathogenesis.Trovato A, Siracusa R, Di Paola R, Scuto M, Fronte V, Koverech G, Luca M, Serra A, Toscano MA, Petralia A, Cuzzocrea S, Calabrese V. - Neurotoxicology. 2016 Mar; 53:350-358.

Infections of cardiovascular implantable electronic devices: 14 years of experience in an Italian hospital.Salmeri M, Sorbello MG, Mastrojeni S, Santanocita A, Milazzo M, Di Stefano G, Scalia M, Addamo A, Toscano MA, Stefani S, Mezzatesta ML. - Infez Med. 2016 Jun 1;24(2):131-6.

Colistin Increases the Cidal Activity of Antibiotic Combinations Against Multidrug-Resistant Klebsiella pneumoniae: An In Vitro Model Comparing Multiple Combination Bactericidal Testing at One Peak Serum Concentration and Time-Kill Method.Mezzatesta ML, Caio C, Gona F, Zingali T, Salerno I, Stefani S. - Microb Drug Resist. 2016 Jul;22(5):360-3.

Growthandadhesionto HT-29 cells inhibition of Gram-negatives by Bifidobacterium longum BB536 e Lactobacillus rhamnosus HN001 alone and in combination. Inturri R, Stivala A, Furneri PM, Blandino G. - Eur Rev Med Pharmacol Sci. 2016 Dec;20(23):4943-4949.

Editorial.Stefani S, Xu YC, Hsueh PR, Gould IM. - J Glob Antimicrob Resist. 2017 Mar;8:A1.

The risk of HCV infection among health-care workers and its association with extrahepatic manifestations.Garozzo A, Falzone L, Rapisarda V, Marconi A, Cinà D, Fenga C, Spandidos DA, Libra M. - Mol Med Rep. 2017 May;15(5):3336-3339.

In vitro activity of fosfomycin trometamol and other oral antibiotics against multidrug-resistant uropathogens.Mezzatesta ML, La Rosa G, Maugeri G, Zingali T, Caio C, Novelli A, Stefani S. - Int J Antimicrob Agents. 2017 Jun;49(6):763-766.

Geo-helminthiasis among migrants in Sicily: a possible focus for re-emerging neurocysticercosis in Europe.Patamia I, Nicotra P, Amodeo D, Giuliano L, Cicero CE, Nicoletti A. - Neurol Sci. 2017 Jun;38(6):1105-1107. doi: 10.1007/s10072-017-2899-4.

Blood-Brain Barrier in a Haemophilus influenzae Type a In Vitro Infection: Role of Adenosine Receptors A2A and A2B.Caporarello N, Olivieri M, Cristaldi M, Scalia M, Toscano MA, Genovese C, Addamo A, Salmeri M, Lupo G, Anfuso CD. - Mol Neurobiol. 2017 Sep 18.

Chemical and biological properties of the novel exopolysaccharide produced by a probiotic strain of Bifidobacterium longum.Inturri R, Molinaro A, Di Lorenzo F, Blandino G, Tomasello B, Hidalgo-Cantabrana C, De Castro C, Ruas-Madiedo P. - Carbohydr Polym. 2017 Oct 15;174:1172-1180.

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Biological properties and production of bacteriocins-like-inhibitory substances by Lactobacillus sp. strains from human vagina.Fuochi V, Cardile V, Petronio Petronio G, Furneri PM. - J Appl Microbiol. 2018 Nov 30. doi: 10.1111/jam.14164.

Bactericidal activity of ceftobiprole combined with different antibiotics against selected Gram-positive isolates.Campanile F, Bongiorno D, Mongelli G, Zanghì G, Stefani S. - Diagnostic Microbiology and Infectious Disease. Volume 93, Issue 1, January 2019, Pages 77-81.

Colistin Resistant A. baumannii: Genomic and Transcriptomic Traits Acquired Under Colistin Therapy.Cafiso V, Stracquadanio S, Lo Verde F, Gabriele G, Mezzatesta ML, Caio C, Pigola G, Ferro A, Stefani S. - Front Microbiol. 2019 Jan 7;9:3195.

In vitro evaluation of biological activities of Orobanche crenata Forssk. leaves extract.Genovese C, Acquaviva R, Ronsisvalle S, Tempera G, Antonio Malfa G, D’Angeli F, Ragusa S, Nicolosi D. - Nat Prod Res. 2019 Jan 23:1-5.

Diagnostic surveillance by Candida albicans germ tube antibody in intensive care unit patients.Trovato L, Astuto M, Castiglione G, Scalia G, Oliveri S. - J Microbiol Immunol Infect. 2019 Mar 11. pii: S1684-1182(18)30431-6. doi: 10.1016/j.jmii.2019.02.001.

Immunomodulatory Effects of Bifidobacterium longum W11 ProducedExopolysaccharide on Cytokine Production.Inturri R, Mangano K, Santagati M, Intrieri M, Di Marco R, Blandino G. - Curr Pharm Biotechnol. 2017 Dec 26.

Differentially Enhancing Effects of Long-term Treatment with Serrazyme, Boswellia and Pine on Seminal Bacterial Detection in Patients with Chronic Bacterial or Inflammatory Prostatitis, Probably Related to Several Degrees of Bacterial Adherence.Vicari E, Malaguarnera G, Vicari B.O, Salmeri M, Salemi M, & Castiglione R. - (2018). Current clinical pharmacology, 13(3), 183-189

Genetic organization of Streptococcus salivarius 24SMBc blp-like bacteriocin locus.Santagati M, Scillato M, Stefani S. - Front Biosci (Schol Ed). 2018 Jan 1;10:238-247

A Method for Efficient Loading of Ciprofloxacin Hydrochloride in Cationic Solid Lipid Nanoparticles: Formulation and Microbiological Evaluation.Pignatello R, Leonardi A, Fuochi V, Petronio Petronio G, Greco AS, Furneri PM. Nanomaterials (Basel). 2018 May 6;8(5). pii: E304.

Type M Resistance to Macrolides Is Due to a Two-Gene Efflux Transport System of the ATP-Binding Cassette (ABC) Superfamily.Iannelli F, Santoro F, Santagati M, Docquier JD, Lazzeri E, Pastore G, Cassone M, Oggioni MR, Rossolini GM, Stefani S, Pozzi G. - Front Microbiol. 2018 Jul 31;9:1670.

Burden of Rifampicin - and Methicillin-Resistant Staphylococcus aureus in Italy. Bongiorno D, Mongelli G, Stefani S, Campanile F. - Volume 24, Issue 6, July-August 2018, Pages 732-738.

Evaluation of isavuconazole MIC strips for susceptibility testing of Aspergillus and Scedosporium species.Trovato L, Scalia G, Palermo CI, Costanzo CM, Oliveri S. - Med Mycol. 2018 Aug 14.

Biological properties of itraconazole-SLN.Fuochi, V., Carbone, C., Petronio Petronio, G., Avola, R., Tibullo, D., Giallongo, C., Puglisi, F., Patamia I, Pignatello R, Furneri P.M. - Biointerface Research in Applied Chemistry, Volume 8, Issue 5, 15 October 2018, Pages 3624-3627

Neonatal HCMV-related polymicrogyria in seroimmune women: What is the optimal pregnancy management? Zavattoni M, Lombardi G, Garofoli F, Scalia G, Rizzo A, Angelini M, Sarasini A, Furione M, Baldanti F. - J Clin Virol. 2018 Nov;108:141-146.



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Section of Microbiology- Microb Drug Resist. 2016 Jul;22(5):360-3. Growthandadhesionto HT-29 cells inhibition of Gram-negatives by Bifidobacterium longum BB536 e Lactobacillus rhamnosus