2012-Present Genomics associate specialist Nestlé Institute of Health Sciences genomics platform – SwitzerlandDesign / Manage / Perform / Report Genomics experiments (NIHS Functional Genomics core)

2008-2011 Post doctoral fellowshipProf. D. Trono's laboratory – EPFL – Switzerland)Characterization of KAP1/TRIM28, a master regulator of epigenetic control

2005-2008 Post doctoral fellowshipProf. D. Schümperli's laboratory – UNIBE – SwitzerlandDevelopment of tools dedicated to SMN2 exon 7 reinclusionImprovement of modified U7 snRNA-based splicing correction strategies

2001-2005 PhD trainingDr. O. Danos gene transfer department – Genethon – FranceDevelopment of a regulatable polyadenylation signalCharacterisation of the FGF1 IRES flexibilityIdentification of RNA sequences with high affinity for CUG-BP1

2000-2001 Post graduation trainingDr. O. Danos gene transfer department – Genethon – FranceAptamer regulation of cap dependent initiation of translation

2000 Engineering school final study project (14 weeks)Althea Technologies – La Jolla – CA – USABio-distribution studies of transgene expression using Taqman technology

2014 Project Management training (QA, Prince2 Foundation & Practitioner - for Nestlé)

2013-Present First-aid continuous training (FormaMed): BLS-AED, Chemical risks, trauma …

2007 Swiss animal experimentation licence (module 1)Flow Cytometry training course (University of Bern – Switzerland)

2001-2005 PhD thesis defense on June 2005 - With honoursLaboratory: Dr. O. Danos Gene transfer department – Genethon – FranceUniversity: Paris VII, B2T "Biotechnology and therapeutics" section – Paris – France

2002 French animal experimentation licence level 1 (highest level)

2001 Post graduation (master thesis) - With honoursLaboratory: Dr. O. Danos Gene transfer department – Genethon – FranceUniversity: INSA – Toulouse – France

1995-2001 INSA (5 years engineering school) Graduated first – Toulouse – France Engineer ing science courses (physics, mathematics, mechanics …)Biology courses: general biology and biochemical engineering

EXPERIENCE

EDUCATION

Great interest in challenging scientific projects Good balance between project driven and technology driven RD High level technical expertise in genomics (Technologies / Applications / Analysis)

Julien Marquis508D rue Voltaire01220 Divonne les BainsFRANCE

Date of birth: October 21st 1978Married – 2 childrenNationality: French

nestle.com+33 (0)9 80 42 86 43+41 (0)79 380 93 06

French: Native languageEnglish: Full professional proficiencySpanish: Correct conversational abilities

Project management Team management, scheduling, budgeting, reporting ...

Genomics Multiple platforms: Illumina / PacBio / Affymetrix / Agilent / Fluidigm / NanostringMultiple applications: RNA/DNA/Exome sequencing, Microarrays, Biomark HD ...

IT Basic knowledge of genomics data analysis resources (Unix, RStudio Bioconductor)

General Biology Advanced design skills for cloning, quantitative RT-PCR, ChIP, SELEXCell culture, shRNA and gene expression analysis (qPCR, FACS, Western blot ...)Retroviral and lentiviral vector production and titrationHistology: Tissue preparation (fixation, slicing), immunohistochemistry, confocal microscopy

Mouse work Care-taking and basic interventions (anaesthesia, blood withdrawal, injection, perfusion …)

Ferrarini A, Xumerle L, Griggio F, Garonzi M, Cantaloni C, Centomo C, Vargas SM, Descombes P, Marquis J, Collino S, et al: The Use of Non-Variant Sites to Improve the Clinical Assessment of Whole-Genome Sequence Data. PLoS One 2015, 10:e0132180.

Atger F, Gobet C, Marquis J, Martin E, Wang J, Weger B, Lefebvre G, Descombes P, Naef F, Gachon F: Circadian and feeding rhythmsdifferentially affect rhythmic mRNA transcription and translation in mouse liver. Proc Natl Acad Sci U S A 2015, 112:E6579-6588.

Rowe HM, Friedli M, Offner S, Verp S, Mesnard D, Marquis J, Aktas T, Trono D: De novo DNA methylation of endogenous retroviruses isshaped by KRAB-ZFPs/KAP1 and ESET. Development 2013, 140:519-529.

Marquez C, Poirier GL, Cordero MI, Larsen MH, Groner A, Marquis J, Magistretti PJ, Trono D, Sandi C: Peripuberty stress leads to abnormal aggression, altered amygdala and orbitofrontal reactivity and increased prefrontal MAOA gene expression. Transl Psychiatry2013, 3:e216.

Barde I, Rauwel B, Marin-Florez RM, Corsinotti A, Laurenti E, Verp S, Offner S, Marquis J, Kapopoulou A, Vanicek J, Trono D: A KRAB/KAP1-miRNA cascade regulates erythropoiesis through stage-specific control of mitophagy. Science 2013, 340:350-353.

Rowe HM, Jakobsson J, Mesnard D, Rougemont J, Reynard S, Aktas T, Maillard PV, Layard-Liesching H, Verp S, Marquis J, et al: KAP1 controls endogenous retroviruses in embryonic stem cells. Nature 2010, 463:237-240.

Meyer K, Marquis J, Trub J, Nlend Nlend R, Verp S, Ruepp MD, Imboden H, Barde I, Trono D, Schumperli D: Rescue of a severe mouse model for spinal muscular atrophy by U7 snRNA-mediated splicing modulation. Hum Mol Genet 2009, 18:546-555.

Marquis J*, Kampfer SS*, Angehrn L, Schumperli D: Doxycycline-controlled splicing modulation by regulated antisense U7 snRNAexpression cassettes. Gene Ther 2009, 16:70-77.

Scholl R*, Marquis J*, Meyer K, Schumperli D: Spinal muscular atrophy: position and functional importance of the branch site preceding SMN exon 7. RNA Biol 2007, 4:34-37.

Marquis J*, Meyer K*, Angehrn L, Kampfer SS, Rothen-Rutishauser B, Schumperli D: Spinal muscular atrophy: SMN2 pre-mRNA splicingcorrected by a U7 snRNA derivative carrying a splicing enhancer sequence. Mol Ther 2007, 15:1479-1486.

Marquis J, Paillard L, Audic Y, Cosson B, Danos O, Le Bec C, Osborne HB: CUG-BP1/CELF1 requires UGU-rich sequences for high-affinitybinding. Biochem J 2006, 400:291-301.

Prof. Didier TronoEcole Polytechnique Fédérale de LausanneLausanne, SwitzertlandXXX+41 (0)21 693 17 51

Prof. Daniel SchümperliUniversity of BernBern, SwitzertlandXXX+41 (0)31 631 46 75

Dr. Patrick DescombesNestlé Institute of Health SciencesLausanne, SwitzerlandXXX+41 (0)21 632 61 15

SKILLS

BIBLIOGRAPHY

LANGUAGE

Football: Player in different clubs for more than 30 years

Outdoor activities: Trail running, Orienteering, Climbing …

INTERESTS

REFERENCES