Séminaire des nouveaux arrivants – UMR8601

Amit Kumar

Laboratoire de Chimie et Biochimie Pharmacologiques et

Toxicologiques

Faculté des Sciences Fondamentales et Biomédicales

Université Paris Descartes

07/2009 – 06/2011 Master of science

Industrial chemistry (Pharmaceutical chemistry)

Guru nanak khalsa college Yamunanagar

(Kurukshetra University, Kurukshetra)

Haryana, INDIA

07/2005 – 05/2009 Bachelor of science

Guru nanak khalsa college Yamunanagar

(Kurukshetra University, Kurukshetra)

Haryana, INDIA

08/2011 – 11/ 2011 Master Thesis

R&D on constituent of chemotherapeutic agents

Teva API india Ltd. Greater Noida U.P. INDIA

Curriculum vitae

1

Industrial Experience

Research Trainee

Teva API India Ltd.

2G, 2H, 2I, Ecotech – II

UdyogVihar, Greater Noida, UP – 201 306

12/2011 -31/2012

Acquired skills:

Impurity synthesis and isolation of APIs and starting materials

01/2013 -06/2014 Research Associate

Teva API India Ltd.

2G, 2H, 2I, Ecotech – II

UdyogVihar, Greater Noida, UP – 201 306

Acquired skills:

Impurity synthesis and isolation of APIs and starting materials

including preparation of reference standard & trouble shooting of APIs 2

JOB WORK:

Synthesized and isolation impurities of different APIs, starting

materials, and intermediates: Anti hypertensives, Diuretics,

Opthalmic & psycho-actives, Antibiotics, Dyslipidemia drugs etc;

R&D & trouble shooting of process and preparation of reference

standards;

Technology transfer up to piloting.

06/2014 -03/2015

Research Scientist ( Executive)

Micro labs Api ltd.

Division 43-45 Kiadb Jigni Bomansandra link road

industrial area phase ii Banglore,INDIA

Responsibilites: Management & teamwork

Research and development of APIs, trouble shooting, impurity

synthesis and isolation of APIs

3

1. Isolation and synthesis of known and unknown

impurities of different APIs and intermediates

For example:

4

i)

ii)

5

iii)

iv) More examples:

6

For example:

2. R&D & trouble shooting of

process & technology transfer

Thesis project

Development of remotely controllable polymersomes for

image guided drug delivery deep within the body

Directeurs de thèse : Pr. Hamid Dhimane & Dr. Peter I. Dalko

Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques

Faculté des Sciences Fondamentales et Biomédicales

Université Paris Descartes

7

ENSCBP-University of Bordeaux

Laboratoire de Chimie des Polymères

Organiques

Polymersomes

USPCI, UFR Biomédicale de Santé,

Laboratoire de Chimie et Biochimie

Pharmacologiques et Toxicologiques,

Redox probes

S. Lecommandoux

P. I. Dalko

H. Dhimane

Organic Synthesis

Polymer Chemistry

Bioimaging, Biology

B.-T. Doan

D. Scherman 2D and 3D imaging Biodistribution, Tumor models

Laboratoire d'Optique Appliquée

ENSTA-ParisTech,

Ecole Polytechnique

P. Zeitoun

X-Ray

ParisTech, ENSCP,

Unité de Technologies Chimiques et

Biologiques pour la Santé

Broad band and coherent X-ray 8

THE NANOMEDICINE RESEARCH

AGENDA:

Nanodiagnostics: early and accurate

diagnostics

-biosensors and miniaturized devices

-targeted imaging agents to highlight of disease

Targeted Drug Delivery: on the spot

-bring the drug to the target site and monitor its

impact

Regenerative Medicine: stimulated

repair

-help the body to (re)built organs or systems

9

NANOVECTORS AS THERAPEUTICS

Liposomes Antibodies and their conjugates Viral vectors Polymeric micelles

Dendrimers and Dendrons Block copolymers Nanoparticles Polymer-proteins conjugates

Nanovectors

10

11

Development of remotely controllable polymersomes for

image guided drug delivery deep within the body

Petit, M.; Bort, G.; Doan, B.-T.; Sicard, C.; Ogden, D.; Scherman, D.; Ferroud, C.; Dalko, P. I. Angew. Chem. Int. Ed.

2011, 50, 9708 –9711 (cover of the week).

Petit, M. ; Bort, G.; Sicard, C.; Ogden, D.; Dalko, P. I.. IP n°10290323.4, 2010

Can be controlled precisely

in terms of the energy and space

WIREs Nanomed Nanobiotechnol 2012, 4:525–546

H. De Oliveira, J. Thevenot, S. Lecommandoux,

X-ray Tissue /Cell

Stimuli-responsive drug delivery

12

Principle of the Auger electron generation

13

N

N

N N

NH

HN

O

OO

H

17

O

O

OO

44I

Block-copolymer

N

N

N N

NH

HN

O

OO

H

17

O

O

OO

44I

Block-copolymer

N

N

N N

NH

HN

O

OO

H

17

OH

I

gamma-PBLG

O

OO

44

HO

Peg-COOH

Redox fragmentation of the

diblock amphiphile

e _

14

Synthesis of the redox co-polymer

NH

O

O

O

OBn

O

H2N N3 N3 NH

HN

O

OBnO

+ Hn

DMF, 40 oC

18 Hrs

Hydrophilic block:

BrO

O

+60% NaH

THF

OHO

O

43

OO

O

44 O

20% TFA

in DCM

OHO

O

44 Ot-Butyl

Bromoacetate PEG-COOH

Hydrophobic block:

15

NOH

Br

+ NO

Cl O

OO

44

HO

O

OO

44

EDC, DMAP

Dry DCM

gamma

PEG-COOH

gamma-PEG

NO

Cl O

OO

44

gamma-PEG

N3 NH

HN

O

OO

H30

N

N

N N

NH

HN

O

OO

H30

O

O

OO

44I

CuI, DIPEA

dry THF+

Azido-PBLG

PEG-gamma-PBLG

Synthesis of the redox co-polymer

16

17

Polymersomes

1

2

3

45 6

7

8

9

1 10

2

3

45 6

7

8

9

11

Organic

Solvent

Water Controlled

flow rate Organic (10 % v/v

Water (90 % v/v)

Copolymer

+ drug

+ nanoparticles

Micelles

OUTSIDE STORY