56
NEW Beckman Coulter Avanti J-26 S Milestone Microwave Digestion Analytik Jena HR-CS AAS - Graphite furnace NEW Radleys Findenser EDITION SPÉCIALE LABORAMA 20-21/03/2014 BRUSSELS KART EXPO Acquisition d'ARDESTA Société spécialisée dans le mobilier de laboratoire Mars 2014 Issue 97 I www.analis.be

Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

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Page 1: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

NEW Beckman Coulter Avanti J-26 S

Milestone Microwave Digestion

Analytik Jena HR-CS AAS - Graphite furnace

NEW Radleys Findenser

Edition speacutecialE

laborama

20-21032014

brussEls kart Ex

po

Acquisition dardestaSocieacuteteacute speacutecialiseacutee dans le mobilier de laboratoire

Mars 2014 Issue 97 I wwwanalisbe

Laborama2 0 amp 2 1 m a r s 2 0 1 4C o M E t o S E E u S

B R u S S E L S K A R t E X P oG R o o t - B i J G A A R D E NW W W L A B o R A M A B E

inscription gratuite en ligne Consultez notre site W W W A N A L i S B E AElig Cliquez sur le logo Laborama agrave droite sur la home page

Analis stand Hall 2 D2Venez deacutecouvrir nos nouvelles technologies amp rencontrer nos speacutecialistes

Vu le succegraves du salon Laborama les Halls 1 et 2 du Brussels Kart Expo sont mobiliseacutes avec lrsquoentreacutee via le Hall 1 pour accueillir plus de 130 exposants

Info pratique amp plan drsquoaccegraves sur W W W L A B o R A M A B E

une eacutequipe agrave votre service

Industry 972

Nouvelle acquisition pour Analis ArdestA

Une association fructueuse pour vous offrir davantage de solutions inteacutegreacutees pour vos projets de mobiliers de laboratoire

Analis est heureux de vous annoncer lrsquoacquisition de la socieacuteteacute ARDEStA speacutecialiseacutee dans la conception la fabrication et lrsquoinstallation de mobiliers de laboratoire depuis plus de 40 ans

ARDESTA est une socieacuteteacute belge qui offre du mobilier de laboratoire tant standard que sur mesure pour reacutepondre speacutecifiquement agrave vos besoins et pour srsquoadapter aux situations les plus exigeantes Lrsquoentreprise possegravede de nombreuses reacutefeacuterences de qualiteacute chez des clients priveacutes et publics

ARDESTA propose divers types de plans de travail meubles eacutetagegraveres et systegravemes drsquoextraction avec toutes les connections souhaiteacutees eau gaz vide eacutelectriciteacute Diffeacuterents mateacuteriaux de construction sont disponibles ainsi qursquoun choix de coloris Son personnel expeacuterimenteacute prend agrave cœur de reacutealiser un travail soigneacute

En exclusiviteacute Ardesta propose une sorbonne agrave recirculation la laquoG Flowraquo issue drsquoune technologie innovante et eacutecologique deacuteveloppeacutee en parte-nariat avec le fabricant franccedilais ERLAB Les avantages de ce modegravele de hotte agrave aspiration reacutesident dans sa rapiditeacute drsquoinstallation et son tregraves faible coucirct de mise en œuvre car aucune gaine technique nrsquoest neacuteces-saire Son coucirct drsquoexploitation est minimiseacute par sa tregraves faible consomma-tion drsquoeacutenergie eacutelectrique mais aussi par lrsquoeacuteconomie reacutealiseacutee en termes de chauffage ou de climatisation En effet cette hotte agrave recirculation rejette une tregraves faible quantiteacute drsquoair de frigories ou de calories

La synergie entre ANALIS et ARDESTA vous permet de trouver lrsquoexpeacuterience et les compeacutetences reacuteunies pour vous proposer des solutions tregraves complegravetes pour vos projets de reacutenovation ou de deacuteveloppement et pour de nouvelles installations de laboratoire

Comme ANALIS ARDESTA partage les mecircmes valeurs drsquoexcellence et de service crsquoest donc logiquement que le rapprochement des deux socieacuteteacutes a vu le jour

Contact infoardestabe

+32 (0)4 286 61 00

wwwanalisbeARDEStA

Industry 97 3

A n a l i s amp A r d e s t a

SommaireCentrifugation07 Particle Characterization amp Centrifugation - Nanotoxicity I beckman coulter

10 Beckman Coulter Avanti J-26S XP I beckman coulterLa centrifugeuse Avanti J-26S permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils

Particle Characterization12 Particle Characterization - Nanotube I beckman coulter

Elemental analysis15 Short Application HR-CS AAS - Graphite furnace I analytik Jena

Determination of Se Sb Pb and Sn in natural water and mud samples

Microwave Sample Prep16 Single Reaction Chamber (SRC) I milestone

Digestion in a benchtop package

Lab Reactor - Synthesis18 NEW Reactor-Ready Pilot in detail I radleys

It is the attention to detail and wealth of innovative features that makes Reactor-Ready Pilot so much better

20 NEW Findenser - Minimises risk of flooding in the laboratory I radleysPerforms as well as a water condenser

Capillary Electrophoresis23 Analysis of Casein and Whey Protein in Whole 2 and Skim Milk I beckman coulter

Liquid Handling28 Analis - Pipette Calibration Service

Depuis plus de 20 ans Analis propose un service de calibration de pipettes

29 Artel PCSreg - Comment ameacuteliorer la qualiteacute de vos manipulations de liquide I artelComment obtenir des reacutesultats toujours preacutecis reproductibles et traccedilables selon les normes NIST

Quality Control amp Packaging Testing30 texte intro I techlab - rdm - presens - lloyd - chatillon - karg

Color Measurement32 Analyse de couleur et de brillance controcircle de couleur visuel et mesure de leacutepaisseur I byk

97Mars 2014

Vous pouvez teacuteleacutecharger nos Flashmag agrave lURL wwwanalisbeflashmag

Coordination

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Production

Seacuteverine Firla

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Tous les prix et offres indiqueacutes sont HTVA en euro - valables du 01032014 au 31052014 sauf erreur drsquoimpression RPM NAMUR 0430744435

In collaboratIon wIth the company bInDer JUlabo amp thermconcept

ANALIS organize a one day free seminar on THERMOSTATISATION amp TEMPERATURE CONTROL TECHNOLOGIELocation ANALIS SUARLEE - Date May 15th 2014

Registration on wwwanalisbeTHERMOSTATISATION

Powder Flow Testing34 New and fast methods to determine powder rheology in rampd and quality control blend of sugars I APTIS

37 Using a shear cell tester to characterize flow agents in fine powders I BRookFIELD

Viscosimetry40 Advancements in Instrumentation for Viscosity Measurements I BRookFIELD

Freeze Drying42 Pourquoi choisir un lyophilisateur MArtIN CHrIst I MARTIN CHRIST

Thermostatisation44 NeW La nouvelle geacuteneacuteration drsquoincubateurs et de fours I MEMMERT

46 Materials testing at a high level I BINDER

48 simulation environnementale I BINDERSeacutechage amp chauffage

Product view New amp Promo06 Microcentrifugeuse NeW

BECKMAN COULTER I Seacuterie 20

11 Capteur drsquooxygegravene NeWpRESENS I Fibox 4 amp Fibox 4 trace

17 Microwave digestion MILESTONE I UltraWave

22 Capillary electrophoresis BECKMAN COULTER I pA800plus

27 Pipette mono- et multicanale ACtIoNHTL I Discovery pro

30 Polymers amp Plastics testing NeWKARG

31 Hardness testingINNOVATEST

33 Banc drsquoessaisLLOyDCHATILLON

45 Laboratory chamber amp tube furnaces NeWTHERMCONCEpT

50 Pompe peacuteristaltique bidirectionelle NeWISMATEC I Reglo ICC

51 Balance ProMoOHAUS I pioneertrade

51 Balance NeWOHAUS I Explorertrade

52 Cryostat JULABO ProMo

53 systegraveme de reacutegulation de tempeacuterature NeWJULABO I presto W91 et W92

53 systegraveme de reacutegulation de tempeacuteratureJULABO I presto W80

54 Laveurs-deacutesinfecteurs MIELE CoNCoUrs

55 Hotte agrave filtration ERLAB56 Microscopes NIKON NeW

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Rapides et polyvalentes les nouvelles seacuteries de microcentrifugeuses haute performance Microfuge 2020R de Beckman Coulter vous offrent haute capaciteacute et haute vitesse avec une grande faciliteacute drsquoutilisation

Ces centrifugeuses de paillasse sont preacuteciseacutement conccedilues pour reacutepondre aux besoins de vos applications la preacuteparation des proteacuteines et acides nucleacuteiques granulation les extractions les purifications les concentrations les seacuteparations de phase et de liaison au reacutecepteur et la seacutedimentation rapide des proteacuteines les particules et les deacutebris cellulaires preacutecipiteacutes

Polyvalence pour reacutepondre agrave vos applications

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Beckman Coulter Application NoteParticle Characterization amp Centrifugation Nanotoxicity

Abstract

Nanomedicine is a field common to materials scientists chemists biomedical engineers biologists and medical scientists The following application note will help researchers in this field gain insight into the inherent shortcomings that are present in traditional cellular toxicity assays and how these shortcomings are magnified when the toxicity assay involves nanomaterial Researchers will also benefit from understanding how Beckman Coulter centrifugation and particle characterization instruments can significantly improve the accuracy and workflow in both preparing nanoparticles and assessing the nanoparticles in vitro toxicity

Introduction

Nanotechnology biomedicine is an emerging field still in its nascent phase Nanoparticles including quantum dots (QDs) carbon nanotubes (CNTs) and graphene have many unique spectral features that have specific application for in vivo imaging and drug delivery1 QDs23 CNTs1 and graphene1 all fluoresce in the advantageous biological window allowing for deeper imaging with better sensitivity45 the strongly red-shifted Raman signal of CNTs has also been exploited for in vivo imaging6 High surface area and strong light absorption of CNTs and graphene also make them excellent drug delivery and photothermal therapy agents1 However toxicity characterization of biologically focused nanomaterial poses a challenge to researchers because of these unique properties

Figure 1 Images of SWCNT Optical images of single-walled carbon nanotube (a) without centrifugation and (b) with ultracentrifugation for two minutes at 55000 RpM (~131000 x g) Note the presence of black aggregated SWCNT in the sample that was not ultracentrifuged

The intrinsic fluorescence and absorption of each of these nanomaterials overlap with the absorption and fluorescence region used typically in vitro cell toxicity assays leading to inaccurate and misleading results7-9 Even more confounding is the fact that absorption fluorescence and toxicity1011 properties of these materials are greatly altered when they are aggregated together compared with individually solubilized thus toxicity studies comparing aggregated vs non-aggregated have systemic bias Aggregated nanoparticles are typically removed by long centrifugation processes ie CNTs typically undergo six-hour centrifugations at 22000 x g to pellet aggregates12-16 This application note highlights how the Beckman Coulter Optima MAX-Xp ultracentrifuge was used to effectively reduce the time to remove the aggregated nanotubes while the Vi-CELL XR from Beckman Coulter quantified cell toxicity in the presence of single-walled carbon nanotubes (SWCNT)

Carbon Nanotube Preparation

Single-walled carbon nanotubes (Sigma-Aldrich) were mixed with 02 1 2-Distearoyl-phospha tidylethanolamine-methyl-polyethyleneglycol(DSpE-mpEG 5 kDa molecular weight Laysan Bio) in 10 mL of water The solution was bath-sonicated for 30 minutes to create well-dispersed carbon nanotubes following previously established procedures Using a TLA-1202 rotor in an Optima MAX-Xp Ultra-centrifuge 5 mL of SWCNT solution was centrifuged in open-top polycarbonate centri-fuge tubes (Beckman Coulter pN 343778) at 22degC 55000 RpM (~131000 x g) for two minutes The top 650 μL of supernatant was collected with care to avoid disturbing the pelleted aggregates The ultracentrifuged SWCNT (referred to as UCFrsquod SWCNT) and the remaining uncentrifuged SWCNT (referred to as As-Made SWCNT) were concentrated using 10 kDa Amicon Ultra 05 mL Centri- fugal Filters (Millipore) with a Beckman Coulter Microfuge 20 microcentrifuge The concen-tration was quantified using a UV-Vis-NIR spectrophotometer (paradigm Molecular Devices) and the established mass extinction coefficient of SWCNTs at 808 nm of 465 Lgcm12 After concentration UCFrsquod SWCNTs and As-Made SWCNTs were dilu-ted using deionized water to concentrations of 06 mgmL 03 mgmL and 006 mgmL

Beckman Coulter Optima Max-Xp Ultracentrifuge

Beckman Coulter Microfuge 20 Microcentrifuge

Industry 97 7wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

C e n t r i f u g a t i o n

Toxicity Assay

MCF-7 breast cancer cells were plated at a density of 008 x 106 per well in a 24-well plate with 900 μL of RPMI10 FBS (Invitrogen) 24 hours before the addition of nanotubes Cell viability and growth were confirmed using one of the wells before the addition of nanotubes 100 μL of SWCNT samples were added to wells on the second day There were six SWCNT groups (n=2group) in total 006 mgmL UCFrsquod SWCNTs 006 mgmL As- Made SWCNTs 003 mgmL UCFrsquod SWCNTs 003 mgmL As-Made SWCNTs 0006 mgmL UCFrsquod SWCNTs and 0006 mgmL As-Made SWCNTs 100 μL of DSPE-mPEG only sample was added to wells serving as a control There were three surfactant buffer control groups (n=2group) in total 02 mgmL DSpE-mpEG 002 mgmL DSpE-mpEG and 0002 mgmL DSpE-mpEG Finally control 1 (n=1) was a complete control with cells left untouched and control 2 (n=1) had 100 μL of sterilized water added After 24 hours all the wells were washed with pBS trypsinized and mixed in 1 mL of pBS for counting in the Vi-CELL XR A new cell type was created in the Vi-CELL XR software to minimize the counting of aggregated nanotubes as cells percentages of viable cells were used to compare cell viability and difference in the two solutions

Figure 2 Cell Imaging MCF-7 cells were imaged under an optical microscope after 24 hours of incubation with SWCNT The cells incubated with either 006 mgmL As-Made SWNT (left image) or 006 mgmL ultracentrifuged SWNT (right image) have not yet reached confluence Black aggregates of SWNT can be seen in the image on the left these aggregates are difficult to wash away without washing away the cells as well The aggregates have absorption and fluorescence properties that will skew traditional toxicity assays

Figure 3 Viability Results At all concentrations ultracentrifuged SWCNT (designated by UC) had minimal toxicity 75 or more of the MCF-7 cells remained viable 24 hours after incubation Contrastingly SWCNT that were not centrifuged and contained aggregated species (designated by AG) had increasing toxicity toward MCF-7 cells that scaled with increasing concentration At a stock concentration of 06 mgmL corresponding to a concentration in solution with cells at 006 mgmL the aggregated SWCNTs had greater than 50 cell death

Figure 4 Size Distribution Data Single-walled carbon nanotubes after sonication in surfactant still have a number of aggregated species Size distribution determined by Dynamic Light Scattering on the DelsaMax pRO showed two broad species (red line) The first size range roughly 100 nm in diameter represents individually solubilized carbon nanotubes The second species containing mostly aggregated carbon nanotubes has a diameter peak closer to one micron in size After a two-minute ultracentrifugation the SWNT demonstrate only a single broad species at 100 nm indicating that virtually all aggregates have been removed This is further indicated by a 59 decrease in polydispersity Interestingly the zeta potential remains unchanged between aggregated and centrifuged carbon nanotubes This is most likely due to the fact that steric repulsion from the poly(ethylene glycol) surfactant provides most of the stability to the carbon nanotubes while electrostatic repulsion does not play a major role

Beckman Coulter Vi-CELL XR Cell Viability Analyser

Beckman Coulter DelsaMax pROparticle Characterization

Industry 978

C e n t r i f u g a t i o n

wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

Reacutefeacuterences

1 Liu Z et al Carbon materials for drug delivery amp cancer therapymdashMaterials Today 147 316-323 (2011)2 Hong G et al In Vivo Fluorescence Imaging with Ag2S Quantum Dots in the Second Near-Infrared RegionmdashAngewandte Chemie 12439 9956-9959 (2012)3 Levina L et al Efficient Infrared-Emitting PbS Quantum Dots Grown on DNA and Stable in Aqueous Solution and Blood plasmamdashAdvanced Materials 1715 1854-1857 (2005)4 Welsher K Sherlock S p and Dai H Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared windowmdashproceedings of the National Academy of Sciences 10822 8943-8948 (2011)5 Smith A M Mancini M C and Nie S Second window for in vivo imagingmdash Nature Nanotechnology 411 710 (2009)6 Zavaleta C et al Noninvasive Raman spectroscopy in living mice for evaluation of tumor targeting with carbon nanotubesmdashNano letters 89 2800-2805 (2008)7 Davoren M et al In vitro toxicity evaluation of single-walled carbon nanotubes on human A549 lung cellsmdashToxicology in vitro an international journal published in association with BIBRA 213 438 (2007)8 Woumlrle-Knirsch J M pulskamp K and Krug H F Oops they did it again Carbon nanotubes hoax scientists in viability assaysmdashNano letters 66 1261-1268 (2006)9 Ren H et al Toxicity of single-walled carbon nanotube How we were wrongmdashMaterials Today 131 6-8 (2010)

10 Wick p et al The degree and kind of agglomeration affect carbon nanotube cytotoxicitymdashToxicology letters 1682 121-131 (2007)11 Lewinski N Colvin V and Drezek R Cytotoxicity of nanoparticlesmdashSmall 41 26-49 (2008)12 Robinson J T et al High performance in vivo near-IR (gt 1 μm) imaging and photothermal cancer therapy with carbon nanotubesmdashNano Research 311 779-793 (2010)13 Donkor A D et al Carbon nanotubes inhibit the hemolytic activity of the pore-forming toxin pyolysinmdashNano Research 27 517-525 (2009)14 Kam N W S et al Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destructionmdash proceedings of the National Academy of Sciences 10233 11600-11605 (2005)15 Robinson J T et al In Vivo Fluorescence Imaging in the Second Near- Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor UptakemdashJournal of the American Chemical Society 13425 10664-10669 (2012)16 Ghafari p Impact of single-walled carbon nanotubes on ciliated protozoa amp bacteriamdashMasterrsquos Thesis University of Waterloo (2009)17 Grabinski C et al Effect of particle dimension on biocompatibility of carbon nanomaterialsmdashCarbon 4514 2828-2835 (2007)

ConclusionDiscussion

Aggregated nanoparticles pose a difficult problem in nano-biomedicine1011 In this study the toxicity of As- Made SWCNTs (which contained visible aggregates) was examined however this data is representative of most nanoparticles The SWCNTs were separated into two groupsmdashone group was As-Made without any purification step to remove aggregates while the second group underwent ultracentrifugation in the Beckman Coulter Optima MAX-Xp ultracentrifuge While centrifugation procedures have been shown to effectively remove aggregated nanoparticles and are a standard for purification of SWCNTs the long centrifugation times (six hours or more) at low speeds (5000 x g to 22000 x g)12-16 are a hindrance to research workflow Our new ultracentrifugation method demonstrates that a two-minute high-speed ultracentrifugation achieves the same biocompatibility and individual solubilized SWCNTs15 as the longer centrifugation timemdasha 180-fold time savings to researchers

Optical images and dynamic light scattering data taken using the DelsaMax pRO are evidence that all aggregated SWCNT have been removed by the rapid ultracentrifugation The toxicity data gathered in this study was possible due to the use of Vi-CELL XR the strong absorption of the aggregates would confound typical MTT and MMp toxicity assays The Vi-CELL XR was programmed to specifically look for spherical cells with defined outlines in a sharply delineated size range ensuring that counting of carbon nanotube aggregates as either viable or dead cells was minimized The Vi-CELL XR optimization is important because of the large number of aggregates present even after cell washing As a control ultracentrifuged nanotubes were run without cells in this trial the Vi-CELL XR did not count a single live or dead cell Aggregates show increased toxicity over ultracentrifuged SWCNT which can be attributed to poor surfactant coverage and larger size of aggregated SWCNT Aggregated SWCNT have more exposed surfactant-free surface this increased surface availability of SWCNT directly contributes to an increase in reactive oxygen species (ROS)17 Furthermore aggregated or As-Made SWCNTs are much larger on a whole as demonstrated by the dynamic light scattering data the increased SWCNT size can block cell-signaling pathways or disrupt cellular action inhibiting cell growth17 Thus it is impor tant that aggregated nanoparticles are removed before being used in vitro or in vivo10

Industry 97 9

C e n t r i f u g a t i o n

wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

NeW Beckman Coulter Avanti J-26s La centrifugeuse Avanti J-26s permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils

La meilleure dans sa cateacutegorieLa centrifugeuse Avanti J-26S Xp permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils Un seul systegraveme traite 6 litres de produit de fermentation en moins de 10 minutes fournit une fraction subcellulaire agrave 82 000 x g centrifuge 24 plaques de microtitration en une fois et seacutepare des populations de cellules vivantes par eacutelutriation

Fonctionnement rapideGracircce agrave la technologie de switched reluctance (SR) drive agrave couple de deacutemarrage eacuteleveacute le temps de cycle est raccourci et il ne faut plus attendre lrsquoeacutechantillon en fin de run En outre le systegraveme de reacuteduction des frictions assure un fonctionnement plus rapide plus silencieux agrave tempeacuterature plus basse et avec une consommation drsquoeacutenergie plus basse que pour les centrifugeuses classiques

Fonctionnement seacutecuriseacuteplusieurs niveaux de bioseacutecuriteacute inteacutegreacutes au niveau des contenants (couvercles joints eacutevents) des rotors (canisters et couvercles seacutecuriseacutes) et de la centrifugeuse (filtres HEPA) A cette bioseacutecuriteacute srsquoajoute lrsquoidentification automatique des rotors pour offrir plus de seacutecuriteacute aux utilisateurs

Toujours precirct agrave lrsquoemploiMecircme si vous nrsquoutilisez pas immeacutediatement tout son potentiel la centrifugeuse Avanti J-26S Xp sera toujours precircte pour reacutepondre aux changements de besoins dans vos recherches Lrsquoassortiment de 26 rotors (parmi lesquels des rotors plus leacutegers des rotors pour centrifuger en continu et eacutelutrier ainsi qursquoune gamme complegravete de rotors BioSafe) fait que lrsquoAvanti J-26S Xp sera sans doute la seule centrifugeuse agrave haute performance dont votre laboratoire aura jamais besoin

Rotor JLA-81000 agrave angle fixe

Utilisation avec la centrifugeuse Avanti J-26S Xp et J-HC

Le systegraveme de rotor composite JLA-81000 avec des pochettes auto-scellan-tes Harvestline traite six litres complets en moins de 10 minutes Sa construc-tion leacutegegravere et les canisters amovibles le rendent tregraves commode pour le traite-ment de gros volumes Les bouteilles J-LITE et leurs couvercles de fermeture sont ideacuteaux pour les reacutecoltes bacteacuteriennes et de cultures cellulaires au deacutepart de fermenteurs pour lrsquoextraction de deacutebris cellulaires pour la purification de gros volumes de virus et polysaccharides dans le cadre de la production de vaccins ou pour la concentration de boues et la clarification de surnageants Ce rotor deacuteveloppe jusqursquoagrave 15 900 x g pour de hauts rendements horaires En permettant des seacuteparations les plus efficaces avec une capaciteacute totale de 6 litres le JLA-81000 peut traiter 36 litres par heure et est de ce fait le plus efficace des rotors disponibles sur le marcheacute

Pour plus dinformations au sujet de ces centrifugeuses et des configurations lieacutees agrave vos applications contactez nos speacutecialistes

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Capteur drsquooxygegravene agrave transmission par fibre optique non-invasif et autonome Le nouveau systegraveme de gestion de donneacutees et le lecteur code-barres acceacutelegravere le processus de mesure- Mesure sans aucun souci gracircce au systegraveme didentification

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Mesure du taux drsquooxygegravene et de la permeacuteabiliteacute de lrsquoemballage

Le transmetteur Fibox 4 trace est lrsquooutil ideacuteal pour les tests dans lrsquoindustrie du packaging et la recherche et le controcircle de qualiteacute des mateacuteriaux La manipulation aiseacutee du systegraveme et la vitesse rapide drsquoeacutetalonnage permettent drsquoeffectuer dans un deacutelai record la mesure de la peacuteneacutetration de loxygegravene et des taux de transmission dans des centaines de contenants et drsquoemballages sans les endommager Un seul scanning du code-barres est

suffisant pour deacutemarrer une mesure preacutecise de traces drsquooxygegravene En combinaison avec le deacutetecteur de type pSt9 qui permet des mesures allant jusquagrave 05 ppm doxygegravene le capteur Fibox permet de deacuteterminer des taux de concentration doxygegravene pour des applications tregraves sensibles ou par exemple pour des emballages conccedilus en mateacuteriaux tregraves denses qui constituent une barriegravere eacuteleveacutee - comme les panneaux disolation sous vide ou de modules de panneaux photovoltaiumlques

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Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

Industry 9712

P a r t i c l e C h a r a c t e r i z a t i o n

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wwwanalisbeDELSAPRotEiN

Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

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wwwanalisbeDELSAPRotEiN

Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

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ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

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single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

Industry 9716wwwanalisbe

Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

Industry 97 17wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

wwwanalisbeREADyPiLot

Industry 9718wwwanalisbe

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

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Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

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Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

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Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

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Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

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C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

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Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

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Industry 97 27

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

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L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

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strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

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Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

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Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

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TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

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Industry 9730wwwanalisbe

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P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

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Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

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Industry 9732wwwanalisbe

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Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

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Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

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720

litre

s

Plag

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re

Fluc

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glab

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A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 2: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Laborama2 0 amp 2 1 m a r s 2 0 1 4C o M E t o S E E u S

B R u S S E L S K A R t E X P oG R o o t - B i J G A A R D E NW W W L A B o R A M A B E

inscription gratuite en ligne Consultez notre site W W W A N A L i S B E AElig Cliquez sur le logo Laborama agrave droite sur la home page

Analis stand Hall 2 D2Venez deacutecouvrir nos nouvelles technologies amp rencontrer nos speacutecialistes

Vu le succegraves du salon Laborama les Halls 1 et 2 du Brussels Kart Expo sont mobiliseacutes avec lrsquoentreacutee via le Hall 1 pour accueillir plus de 130 exposants

Info pratique amp plan drsquoaccegraves sur W W W L A B o R A M A B E

une eacutequipe agrave votre service

Industry 972

Nouvelle acquisition pour Analis ArdestA

Une association fructueuse pour vous offrir davantage de solutions inteacutegreacutees pour vos projets de mobiliers de laboratoire

Analis est heureux de vous annoncer lrsquoacquisition de la socieacuteteacute ARDEStA speacutecialiseacutee dans la conception la fabrication et lrsquoinstallation de mobiliers de laboratoire depuis plus de 40 ans

ARDESTA est une socieacuteteacute belge qui offre du mobilier de laboratoire tant standard que sur mesure pour reacutepondre speacutecifiquement agrave vos besoins et pour srsquoadapter aux situations les plus exigeantes Lrsquoentreprise possegravede de nombreuses reacutefeacuterences de qualiteacute chez des clients priveacutes et publics

ARDESTA propose divers types de plans de travail meubles eacutetagegraveres et systegravemes drsquoextraction avec toutes les connections souhaiteacutees eau gaz vide eacutelectriciteacute Diffeacuterents mateacuteriaux de construction sont disponibles ainsi qursquoun choix de coloris Son personnel expeacuterimenteacute prend agrave cœur de reacutealiser un travail soigneacute

En exclusiviteacute Ardesta propose une sorbonne agrave recirculation la laquoG Flowraquo issue drsquoune technologie innovante et eacutecologique deacuteveloppeacutee en parte-nariat avec le fabricant franccedilais ERLAB Les avantages de ce modegravele de hotte agrave aspiration reacutesident dans sa rapiditeacute drsquoinstallation et son tregraves faible coucirct de mise en œuvre car aucune gaine technique nrsquoest neacuteces-saire Son coucirct drsquoexploitation est minimiseacute par sa tregraves faible consomma-tion drsquoeacutenergie eacutelectrique mais aussi par lrsquoeacuteconomie reacutealiseacutee en termes de chauffage ou de climatisation En effet cette hotte agrave recirculation rejette une tregraves faible quantiteacute drsquoair de frigories ou de calories

La synergie entre ANALIS et ARDESTA vous permet de trouver lrsquoexpeacuterience et les compeacutetences reacuteunies pour vous proposer des solutions tregraves complegravetes pour vos projets de reacutenovation ou de deacuteveloppement et pour de nouvelles installations de laboratoire

Comme ANALIS ARDESTA partage les mecircmes valeurs drsquoexcellence et de service crsquoest donc logiquement que le rapprochement des deux socieacuteteacutes a vu le jour

Contact infoardestabe

+32 (0)4 286 61 00

wwwanalisbeARDEStA

Industry 97 3

A n a l i s amp A r d e s t a

SommaireCentrifugation07 Particle Characterization amp Centrifugation - Nanotoxicity I beckman coulter

10 Beckman Coulter Avanti J-26S XP I beckman coulterLa centrifugeuse Avanti J-26S permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils

Particle Characterization12 Particle Characterization - Nanotube I beckman coulter

Elemental analysis15 Short Application HR-CS AAS - Graphite furnace I analytik Jena

Determination of Se Sb Pb and Sn in natural water and mud samples

Microwave Sample Prep16 Single Reaction Chamber (SRC) I milestone

Digestion in a benchtop package

Lab Reactor - Synthesis18 NEW Reactor-Ready Pilot in detail I radleys

It is the attention to detail and wealth of innovative features that makes Reactor-Ready Pilot so much better

20 NEW Findenser - Minimises risk of flooding in the laboratory I radleysPerforms as well as a water condenser

Capillary Electrophoresis23 Analysis of Casein and Whey Protein in Whole 2 and Skim Milk I beckman coulter

Liquid Handling28 Analis - Pipette Calibration Service

Depuis plus de 20 ans Analis propose un service de calibration de pipettes

29 Artel PCSreg - Comment ameacuteliorer la qualiteacute de vos manipulations de liquide I artelComment obtenir des reacutesultats toujours preacutecis reproductibles et traccedilables selon les normes NIST

Quality Control amp Packaging Testing30 texte intro I techlab - rdm - presens - lloyd - chatillon - karg

Color Measurement32 Analyse de couleur et de brillance controcircle de couleur visuel et mesure de leacutepaisseur I byk

97Mars 2014

Vous pouvez teacuteleacutecharger nos Flashmag agrave lURL wwwanalisbeflashmag

Coordination

Eliane Henri ehianalisbe

Production

Seacuteverine Firla

tirage

2 500 exemplaires

diffusion

Belgique Luxembourg

Analis sanv BELGIQUE

suarleacutee - siegravege social Rue de Neacuteverleacutee 11 - 5020 Suarleacutee TEL 081 25 50 50 - FAX 081 23 07 79

sint-denijs-Westrem Bijenstraat 18 - Entrance C9051 Sint-Denijs-Westrem TEL 09 243 77 10 - FAX 09 220 08 48

w w w a n a l i s b e

Tous les prix et offres indiqueacutes sont HTVA en euro - valables du 01032014 au 31052014 sauf erreur drsquoimpression RPM NAMUR 0430744435

In collaboratIon wIth the company bInDer JUlabo amp thermconcept

ANALIS organize a one day free seminar on THERMOSTATISATION amp TEMPERATURE CONTROL TECHNOLOGIELocation ANALIS SUARLEE - Date May 15th 2014

Registration on wwwanalisbeTHERMOSTATISATION

Powder Flow Testing34 New and fast methods to determine powder rheology in rampd and quality control blend of sugars I APTIS

37 Using a shear cell tester to characterize flow agents in fine powders I BRookFIELD

Viscosimetry40 Advancements in Instrumentation for Viscosity Measurements I BRookFIELD

Freeze Drying42 Pourquoi choisir un lyophilisateur MArtIN CHrIst I MARTIN CHRIST

Thermostatisation44 NeW La nouvelle geacuteneacuteration drsquoincubateurs et de fours I MEMMERT

46 Materials testing at a high level I BINDER

48 simulation environnementale I BINDERSeacutechage amp chauffage

Product view New amp Promo06 Microcentrifugeuse NeW

BECKMAN COULTER I Seacuterie 20

11 Capteur drsquooxygegravene NeWpRESENS I Fibox 4 amp Fibox 4 trace

17 Microwave digestion MILESTONE I UltraWave

22 Capillary electrophoresis BECKMAN COULTER I pA800plus

27 Pipette mono- et multicanale ACtIoNHTL I Discovery pro

30 Polymers amp Plastics testing NeWKARG

31 Hardness testingINNOVATEST

33 Banc drsquoessaisLLOyDCHATILLON

45 Laboratory chamber amp tube furnaces NeWTHERMCONCEpT

50 Pompe peacuteristaltique bidirectionelle NeWISMATEC I Reglo ICC

51 Balance ProMoOHAUS I pioneertrade

51 Balance NeWOHAUS I Explorertrade

52 Cryostat JULABO ProMo

53 systegraveme de reacutegulation de tempeacuterature NeWJULABO I presto W91 et W92

53 systegraveme de reacutegulation de tempeacuteratureJULABO I presto W80

54 Laveurs-deacutesinfecteurs MIELE CoNCoUrs

55 Hotte agrave filtration ERLAB56 Microscopes NIKON NeW

Industry 97 5

NEW MicrocENtrifugEusEsbeckman coulter serie 20

wwwanalisbeMiCRoFuGE20

Rapides et polyvalentes les nouvelles seacuteries de microcentrifugeuses haute performance Microfuge 2020R de Beckman Coulter vous offrent haute capaciteacute et haute vitesse avec une grande faciliteacute drsquoutilisation

Ces centrifugeuses de paillasse sont preacuteciseacutement conccedilues pour reacutepondre aux besoins de vos applications la preacuteparation des proteacuteines et acides nucleacuteiques granulation les extractions les purifications les concentrations les seacuteparations de phase et de liaison au reacutecepteur et la seacutedimentation rapide des proteacuteines les particules et les deacutebris cellulaires preacutecipiteacutes

Polyvalence pour reacutepondre agrave vos applications

- Disponible en deux versions reacutefrigeacutereacutee (Microfuge 20R) et non reacutefrigeacutereacutee (Microfuge 20)

- Le choix parmi quatre options de rotors diffeacuterents - La seacutecuriteacute pour vos eacutechantillons dangereux avec un

rotor BioCertified (option)

Vitesse et capaciteacute augmenteacutees

- Traitez rapidement vos eacutechantillons avec une vitesse maximale de 15000 rpm (20627 x g)

- Centrifugez plus drsquoeacutechantillons par cycle avec une capaciteacute maximale de 36 tubes de 1520 ml

Facile agrave utiliser et agrave inteacutegrer dans votre laboratoire

- Interface utilisateur intuitive qui affiche la vitesse (rpm) et le RCF (XG)

- Reacuteduisez le temps dinstallation avec des programmes deacutefinis par lutilisateur pour des protocoles communs

- Reacuteduction des coucircts deacutenergie avec une efficaciteacute eacutenergeacutetique accrue

Augmentez la vitesse et la capaciteacute tout en reacuteduisant vos coucircts

Plus dinformations sur notre site web

Industry 976wwwanalisbe

Info Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

Beckman Coulter Application NoteParticle Characterization amp Centrifugation Nanotoxicity

Abstract

Nanomedicine is a field common to materials scientists chemists biomedical engineers biologists and medical scientists The following application note will help researchers in this field gain insight into the inherent shortcomings that are present in traditional cellular toxicity assays and how these shortcomings are magnified when the toxicity assay involves nanomaterial Researchers will also benefit from understanding how Beckman Coulter centrifugation and particle characterization instruments can significantly improve the accuracy and workflow in both preparing nanoparticles and assessing the nanoparticles in vitro toxicity

Introduction

Nanotechnology biomedicine is an emerging field still in its nascent phase Nanoparticles including quantum dots (QDs) carbon nanotubes (CNTs) and graphene have many unique spectral features that have specific application for in vivo imaging and drug delivery1 QDs23 CNTs1 and graphene1 all fluoresce in the advantageous biological window allowing for deeper imaging with better sensitivity45 the strongly red-shifted Raman signal of CNTs has also been exploited for in vivo imaging6 High surface area and strong light absorption of CNTs and graphene also make them excellent drug delivery and photothermal therapy agents1 However toxicity characterization of biologically focused nanomaterial poses a challenge to researchers because of these unique properties

Figure 1 Images of SWCNT Optical images of single-walled carbon nanotube (a) without centrifugation and (b) with ultracentrifugation for two minutes at 55000 RpM (~131000 x g) Note the presence of black aggregated SWCNT in the sample that was not ultracentrifuged

The intrinsic fluorescence and absorption of each of these nanomaterials overlap with the absorption and fluorescence region used typically in vitro cell toxicity assays leading to inaccurate and misleading results7-9 Even more confounding is the fact that absorption fluorescence and toxicity1011 properties of these materials are greatly altered when they are aggregated together compared with individually solubilized thus toxicity studies comparing aggregated vs non-aggregated have systemic bias Aggregated nanoparticles are typically removed by long centrifugation processes ie CNTs typically undergo six-hour centrifugations at 22000 x g to pellet aggregates12-16 This application note highlights how the Beckman Coulter Optima MAX-Xp ultracentrifuge was used to effectively reduce the time to remove the aggregated nanotubes while the Vi-CELL XR from Beckman Coulter quantified cell toxicity in the presence of single-walled carbon nanotubes (SWCNT)

Carbon Nanotube Preparation

Single-walled carbon nanotubes (Sigma-Aldrich) were mixed with 02 1 2-Distearoyl-phospha tidylethanolamine-methyl-polyethyleneglycol(DSpE-mpEG 5 kDa molecular weight Laysan Bio) in 10 mL of water The solution was bath-sonicated for 30 minutes to create well-dispersed carbon nanotubes following previously established procedures Using a TLA-1202 rotor in an Optima MAX-Xp Ultra-centrifuge 5 mL of SWCNT solution was centrifuged in open-top polycarbonate centri-fuge tubes (Beckman Coulter pN 343778) at 22degC 55000 RpM (~131000 x g) for two minutes The top 650 μL of supernatant was collected with care to avoid disturbing the pelleted aggregates The ultracentrifuged SWCNT (referred to as UCFrsquod SWCNT) and the remaining uncentrifuged SWCNT (referred to as As-Made SWCNT) were concentrated using 10 kDa Amicon Ultra 05 mL Centri- fugal Filters (Millipore) with a Beckman Coulter Microfuge 20 microcentrifuge The concen-tration was quantified using a UV-Vis-NIR spectrophotometer (paradigm Molecular Devices) and the established mass extinction coefficient of SWCNTs at 808 nm of 465 Lgcm12 After concentration UCFrsquod SWCNTs and As-Made SWCNTs were dilu-ted using deionized water to concentrations of 06 mgmL 03 mgmL and 006 mgmL

Beckman Coulter Optima Max-Xp Ultracentrifuge

Beckman Coulter Microfuge 20 Microcentrifuge

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C e n t r i f u g a t i o n

Toxicity Assay

MCF-7 breast cancer cells were plated at a density of 008 x 106 per well in a 24-well plate with 900 μL of RPMI10 FBS (Invitrogen) 24 hours before the addition of nanotubes Cell viability and growth were confirmed using one of the wells before the addition of nanotubes 100 μL of SWCNT samples were added to wells on the second day There were six SWCNT groups (n=2group) in total 006 mgmL UCFrsquod SWCNTs 006 mgmL As- Made SWCNTs 003 mgmL UCFrsquod SWCNTs 003 mgmL As-Made SWCNTs 0006 mgmL UCFrsquod SWCNTs and 0006 mgmL As-Made SWCNTs 100 μL of DSPE-mPEG only sample was added to wells serving as a control There were three surfactant buffer control groups (n=2group) in total 02 mgmL DSpE-mpEG 002 mgmL DSpE-mpEG and 0002 mgmL DSpE-mpEG Finally control 1 (n=1) was a complete control with cells left untouched and control 2 (n=1) had 100 μL of sterilized water added After 24 hours all the wells were washed with pBS trypsinized and mixed in 1 mL of pBS for counting in the Vi-CELL XR A new cell type was created in the Vi-CELL XR software to minimize the counting of aggregated nanotubes as cells percentages of viable cells were used to compare cell viability and difference in the two solutions

Figure 2 Cell Imaging MCF-7 cells were imaged under an optical microscope after 24 hours of incubation with SWCNT The cells incubated with either 006 mgmL As-Made SWNT (left image) or 006 mgmL ultracentrifuged SWNT (right image) have not yet reached confluence Black aggregates of SWNT can be seen in the image on the left these aggregates are difficult to wash away without washing away the cells as well The aggregates have absorption and fluorescence properties that will skew traditional toxicity assays

Figure 3 Viability Results At all concentrations ultracentrifuged SWCNT (designated by UC) had minimal toxicity 75 or more of the MCF-7 cells remained viable 24 hours after incubation Contrastingly SWCNT that were not centrifuged and contained aggregated species (designated by AG) had increasing toxicity toward MCF-7 cells that scaled with increasing concentration At a stock concentration of 06 mgmL corresponding to a concentration in solution with cells at 006 mgmL the aggregated SWCNTs had greater than 50 cell death

Figure 4 Size Distribution Data Single-walled carbon nanotubes after sonication in surfactant still have a number of aggregated species Size distribution determined by Dynamic Light Scattering on the DelsaMax pRO showed two broad species (red line) The first size range roughly 100 nm in diameter represents individually solubilized carbon nanotubes The second species containing mostly aggregated carbon nanotubes has a diameter peak closer to one micron in size After a two-minute ultracentrifugation the SWNT demonstrate only a single broad species at 100 nm indicating that virtually all aggregates have been removed This is further indicated by a 59 decrease in polydispersity Interestingly the zeta potential remains unchanged between aggregated and centrifuged carbon nanotubes This is most likely due to the fact that steric repulsion from the poly(ethylene glycol) surfactant provides most of the stability to the carbon nanotubes while electrostatic repulsion does not play a major role

Beckman Coulter Vi-CELL XR Cell Viability Analyser

Beckman Coulter DelsaMax pROparticle Characterization

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Reacutefeacuterences

1 Liu Z et al Carbon materials for drug delivery amp cancer therapymdashMaterials Today 147 316-323 (2011)2 Hong G et al In Vivo Fluorescence Imaging with Ag2S Quantum Dots in the Second Near-Infrared RegionmdashAngewandte Chemie 12439 9956-9959 (2012)3 Levina L et al Efficient Infrared-Emitting PbS Quantum Dots Grown on DNA and Stable in Aqueous Solution and Blood plasmamdashAdvanced Materials 1715 1854-1857 (2005)4 Welsher K Sherlock S p and Dai H Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared windowmdashproceedings of the National Academy of Sciences 10822 8943-8948 (2011)5 Smith A M Mancini M C and Nie S Second window for in vivo imagingmdash Nature Nanotechnology 411 710 (2009)6 Zavaleta C et al Noninvasive Raman spectroscopy in living mice for evaluation of tumor targeting with carbon nanotubesmdashNano letters 89 2800-2805 (2008)7 Davoren M et al In vitro toxicity evaluation of single-walled carbon nanotubes on human A549 lung cellsmdashToxicology in vitro an international journal published in association with BIBRA 213 438 (2007)8 Woumlrle-Knirsch J M pulskamp K and Krug H F Oops they did it again Carbon nanotubes hoax scientists in viability assaysmdashNano letters 66 1261-1268 (2006)9 Ren H et al Toxicity of single-walled carbon nanotube How we were wrongmdashMaterials Today 131 6-8 (2010)

10 Wick p et al The degree and kind of agglomeration affect carbon nanotube cytotoxicitymdashToxicology letters 1682 121-131 (2007)11 Lewinski N Colvin V and Drezek R Cytotoxicity of nanoparticlesmdashSmall 41 26-49 (2008)12 Robinson J T et al High performance in vivo near-IR (gt 1 μm) imaging and photothermal cancer therapy with carbon nanotubesmdashNano Research 311 779-793 (2010)13 Donkor A D et al Carbon nanotubes inhibit the hemolytic activity of the pore-forming toxin pyolysinmdashNano Research 27 517-525 (2009)14 Kam N W S et al Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destructionmdash proceedings of the National Academy of Sciences 10233 11600-11605 (2005)15 Robinson J T et al In Vivo Fluorescence Imaging in the Second Near- Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor UptakemdashJournal of the American Chemical Society 13425 10664-10669 (2012)16 Ghafari p Impact of single-walled carbon nanotubes on ciliated protozoa amp bacteriamdashMasterrsquos Thesis University of Waterloo (2009)17 Grabinski C et al Effect of particle dimension on biocompatibility of carbon nanomaterialsmdashCarbon 4514 2828-2835 (2007)

ConclusionDiscussion

Aggregated nanoparticles pose a difficult problem in nano-biomedicine1011 In this study the toxicity of As- Made SWCNTs (which contained visible aggregates) was examined however this data is representative of most nanoparticles The SWCNTs were separated into two groupsmdashone group was As-Made without any purification step to remove aggregates while the second group underwent ultracentrifugation in the Beckman Coulter Optima MAX-Xp ultracentrifuge While centrifugation procedures have been shown to effectively remove aggregated nanoparticles and are a standard for purification of SWCNTs the long centrifugation times (six hours or more) at low speeds (5000 x g to 22000 x g)12-16 are a hindrance to research workflow Our new ultracentrifugation method demonstrates that a two-minute high-speed ultracentrifugation achieves the same biocompatibility and individual solubilized SWCNTs15 as the longer centrifugation timemdasha 180-fold time savings to researchers

Optical images and dynamic light scattering data taken using the DelsaMax pRO are evidence that all aggregated SWCNT have been removed by the rapid ultracentrifugation The toxicity data gathered in this study was possible due to the use of Vi-CELL XR the strong absorption of the aggregates would confound typical MTT and MMp toxicity assays The Vi-CELL XR was programmed to specifically look for spherical cells with defined outlines in a sharply delineated size range ensuring that counting of carbon nanotube aggregates as either viable or dead cells was minimized The Vi-CELL XR optimization is important because of the large number of aggregates present even after cell washing As a control ultracentrifuged nanotubes were run without cells in this trial the Vi-CELL XR did not count a single live or dead cell Aggregates show increased toxicity over ultracentrifuged SWCNT which can be attributed to poor surfactant coverage and larger size of aggregated SWCNT Aggregated SWCNT have more exposed surfactant-free surface this increased surface availability of SWCNT directly contributes to an increase in reactive oxygen species (ROS)17 Furthermore aggregated or As-Made SWCNTs are much larger on a whole as demonstrated by the dynamic light scattering data the increased SWCNT size can block cell-signaling pathways or disrupt cellular action inhibiting cell growth17 Thus it is impor tant that aggregated nanoparticles are removed before being used in vitro or in vivo10

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La meilleure dans sa cateacutegorieLa centrifugeuse Avanti J-26S Xp permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils Un seul systegraveme traite 6 litres de produit de fermentation en moins de 10 minutes fournit une fraction subcellulaire agrave 82 000 x g centrifuge 24 plaques de microtitration en une fois et seacutepare des populations de cellules vivantes par eacutelutriation

Fonctionnement rapideGracircce agrave la technologie de switched reluctance (SR) drive agrave couple de deacutemarrage eacuteleveacute le temps de cycle est raccourci et il ne faut plus attendre lrsquoeacutechantillon en fin de run En outre le systegraveme de reacuteduction des frictions assure un fonctionnement plus rapide plus silencieux agrave tempeacuterature plus basse et avec une consommation drsquoeacutenergie plus basse que pour les centrifugeuses classiques

Fonctionnement seacutecuriseacuteplusieurs niveaux de bioseacutecuriteacute inteacutegreacutes au niveau des contenants (couvercles joints eacutevents) des rotors (canisters et couvercles seacutecuriseacutes) et de la centrifugeuse (filtres HEPA) A cette bioseacutecuriteacute srsquoajoute lrsquoidentification automatique des rotors pour offrir plus de seacutecuriteacute aux utilisateurs

Toujours precirct agrave lrsquoemploiMecircme si vous nrsquoutilisez pas immeacutediatement tout son potentiel la centrifugeuse Avanti J-26S Xp sera toujours precircte pour reacutepondre aux changements de besoins dans vos recherches Lrsquoassortiment de 26 rotors (parmi lesquels des rotors plus leacutegers des rotors pour centrifuger en continu et eacutelutrier ainsi qursquoune gamme complegravete de rotors BioSafe) fait que lrsquoAvanti J-26S Xp sera sans doute la seule centrifugeuse agrave haute performance dont votre laboratoire aura jamais besoin

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Utilisation avec la centrifugeuse Avanti J-26S Xp et J-HC

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Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

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Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

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Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

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ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

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single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

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M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

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M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

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Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

entiegraverementautoclavables(121degC100kPa20min)etpeuventecirctresteacuteriliseacutees

sansrisqueauxUV

Industry 97 27

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

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Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

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Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

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Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

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Page 3: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

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A n a l i s amp A r d e s t a

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Depuis plus de 20 ans Analis propose un service de calibration de pipettes

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Beckman Coulter Application NoteParticle Characterization amp Centrifugation Nanotoxicity

Abstract

Nanomedicine is a field common to materials scientists chemists biomedical engineers biologists and medical scientists The following application note will help researchers in this field gain insight into the inherent shortcomings that are present in traditional cellular toxicity assays and how these shortcomings are magnified when the toxicity assay involves nanomaterial Researchers will also benefit from understanding how Beckman Coulter centrifugation and particle characterization instruments can significantly improve the accuracy and workflow in both preparing nanoparticles and assessing the nanoparticles in vitro toxicity

Introduction

Nanotechnology biomedicine is an emerging field still in its nascent phase Nanoparticles including quantum dots (QDs) carbon nanotubes (CNTs) and graphene have many unique spectral features that have specific application for in vivo imaging and drug delivery1 QDs23 CNTs1 and graphene1 all fluoresce in the advantageous biological window allowing for deeper imaging with better sensitivity45 the strongly red-shifted Raman signal of CNTs has also been exploited for in vivo imaging6 High surface area and strong light absorption of CNTs and graphene also make them excellent drug delivery and photothermal therapy agents1 However toxicity characterization of biologically focused nanomaterial poses a challenge to researchers because of these unique properties

Figure 1 Images of SWCNT Optical images of single-walled carbon nanotube (a) without centrifugation and (b) with ultracentrifugation for two minutes at 55000 RpM (~131000 x g) Note the presence of black aggregated SWCNT in the sample that was not ultracentrifuged

The intrinsic fluorescence and absorption of each of these nanomaterials overlap with the absorption and fluorescence region used typically in vitro cell toxicity assays leading to inaccurate and misleading results7-9 Even more confounding is the fact that absorption fluorescence and toxicity1011 properties of these materials are greatly altered when they are aggregated together compared with individually solubilized thus toxicity studies comparing aggregated vs non-aggregated have systemic bias Aggregated nanoparticles are typically removed by long centrifugation processes ie CNTs typically undergo six-hour centrifugations at 22000 x g to pellet aggregates12-16 This application note highlights how the Beckman Coulter Optima MAX-Xp ultracentrifuge was used to effectively reduce the time to remove the aggregated nanotubes while the Vi-CELL XR from Beckman Coulter quantified cell toxicity in the presence of single-walled carbon nanotubes (SWCNT)

Carbon Nanotube Preparation

Single-walled carbon nanotubes (Sigma-Aldrich) were mixed with 02 1 2-Distearoyl-phospha tidylethanolamine-methyl-polyethyleneglycol(DSpE-mpEG 5 kDa molecular weight Laysan Bio) in 10 mL of water The solution was bath-sonicated for 30 minutes to create well-dispersed carbon nanotubes following previously established procedures Using a TLA-1202 rotor in an Optima MAX-Xp Ultra-centrifuge 5 mL of SWCNT solution was centrifuged in open-top polycarbonate centri-fuge tubes (Beckman Coulter pN 343778) at 22degC 55000 RpM (~131000 x g) for two minutes The top 650 μL of supernatant was collected with care to avoid disturbing the pelleted aggregates The ultracentrifuged SWCNT (referred to as UCFrsquod SWCNT) and the remaining uncentrifuged SWCNT (referred to as As-Made SWCNT) were concentrated using 10 kDa Amicon Ultra 05 mL Centri- fugal Filters (Millipore) with a Beckman Coulter Microfuge 20 microcentrifuge The concen-tration was quantified using a UV-Vis-NIR spectrophotometer (paradigm Molecular Devices) and the established mass extinction coefficient of SWCNTs at 808 nm of 465 Lgcm12 After concentration UCFrsquod SWCNTs and As-Made SWCNTs were dilu-ted using deionized water to concentrations of 06 mgmL 03 mgmL and 006 mgmL

Beckman Coulter Optima Max-Xp Ultracentrifuge

Beckman Coulter Microfuge 20 Microcentrifuge

Industry 97 7wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

C e n t r i f u g a t i o n

Toxicity Assay

MCF-7 breast cancer cells were plated at a density of 008 x 106 per well in a 24-well plate with 900 μL of RPMI10 FBS (Invitrogen) 24 hours before the addition of nanotubes Cell viability and growth were confirmed using one of the wells before the addition of nanotubes 100 μL of SWCNT samples were added to wells on the second day There were six SWCNT groups (n=2group) in total 006 mgmL UCFrsquod SWCNTs 006 mgmL As- Made SWCNTs 003 mgmL UCFrsquod SWCNTs 003 mgmL As-Made SWCNTs 0006 mgmL UCFrsquod SWCNTs and 0006 mgmL As-Made SWCNTs 100 μL of DSPE-mPEG only sample was added to wells serving as a control There were three surfactant buffer control groups (n=2group) in total 02 mgmL DSpE-mpEG 002 mgmL DSpE-mpEG and 0002 mgmL DSpE-mpEG Finally control 1 (n=1) was a complete control with cells left untouched and control 2 (n=1) had 100 μL of sterilized water added After 24 hours all the wells were washed with pBS trypsinized and mixed in 1 mL of pBS for counting in the Vi-CELL XR A new cell type was created in the Vi-CELL XR software to minimize the counting of aggregated nanotubes as cells percentages of viable cells were used to compare cell viability and difference in the two solutions

Figure 2 Cell Imaging MCF-7 cells were imaged under an optical microscope after 24 hours of incubation with SWCNT The cells incubated with either 006 mgmL As-Made SWNT (left image) or 006 mgmL ultracentrifuged SWNT (right image) have not yet reached confluence Black aggregates of SWNT can be seen in the image on the left these aggregates are difficult to wash away without washing away the cells as well The aggregates have absorption and fluorescence properties that will skew traditional toxicity assays

Figure 3 Viability Results At all concentrations ultracentrifuged SWCNT (designated by UC) had minimal toxicity 75 or more of the MCF-7 cells remained viable 24 hours after incubation Contrastingly SWCNT that were not centrifuged and contained aggregated species (designated by AG) had increasing toxicity toward MCF-7 cells that scaled with increasing concentration At a stock concentration of 06 mgmL corresponding to a concentration in solution with cells at 006 mgmL the aggregated SWCNTs had greater than 50 cell death

Figure 4 Size Distribution Data Single-walled carbon nanotubes after sonication in surfactant still have a number of aggregated species Size distribution determined by Dynamic Light Scattering on the DelsaMax pRO showed two broad species (red line) The first size range roughly 100 nm in diameter represents individually solubilized carbon nanotubes The second species containing mostly aggregated carbon nanotubes has a diameter peak closer to one micron in size After a two-minute ultracentrifugation the SWNT demonstrate only a single broad species at 100 nm indicating that virtually all aggregates have been removed This is further indicated by a 59 decrease in polydispersity Interestingly the zeta potential remains unchanged between aggregated and centrifuged carbon nanotubes This is most likely due to the fact that steric repulsion from the poly(ethylene glycol) surfactant provides most of the stability to the carbon nanotubes while electrostatic repulsion does not play a major role

Beckman Coulter Vi-CELL XR Cell Viability Analyser

Beckman Coulter DelsaMax pROparticle Characterization

Industry 978

C e n t r i f u g a t i o n

wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

Reacutefeacuterences

1 Liu Z et al Carbon materials for drug delivery amp cancer therapymdashMaterials Today 147 316-323 (2011)2 Hong G et al In Vivo Fluorescence Imaging with Ag2S Quantum Dots in the Second Near-Infrared RegionmdashAngewandte Chemie 12439 9956-9959 (2012)3 Levina L et al Efficient Infrared-Emitting PbS Quantum Dots Grown on DNA and Stable in Aqueous Solution and Blood plasmamdashAdvanced Materials 1715 1854-1857 (2005)4 Welsher K Sherlock S p and Dai H Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared windowmdashproceedings of the National Academy of Sciences 10822 8943-8948 (2011)5 Smith A M Mancini M C and Nie S Second window for in vivo imagingmdash Nature Nanotechnology 411 710 (2009)6 Zavaleta C et al Noninvasive Raman spectroscopy in living mice for evaluation of tumor targeting with carbon nanotubesmdashNano letters 89 2800-2805 (2008)7 Davoren M et al In vitro toxicity evaluation of single-walled carbon nanotubes on human A549 lung cellsmdashToxicology in vitro an international journal published in association with BIBRA 213 438 (2007)8 Woumlrle-Knirsch J M pulskamp K and Krug H F Oops they did it again Carbon nanotubes hoax scientists in viability assaysmdashNano letters 66 1261-1268 (2006)9 Ren H et al Toxicity of single-walled carbon nanotube How we were wrongmdashMaterials Today 131 6-8 (2010)

10 Wick p et al The degree and kind of agglomeration affect carbon nanotube cytotoxicitymdashToxicology letters 1682 121-131 (2007)11 Lewinski N Colvin V and Drezek R Cytotoxicity of nanoparticlesmdashSmall 41 26-49 (2008)12 Robinson J T et al High performance in vivo near-IR (gt 1 μm) imaging and photothermal cancer therapy with carbon nanotubesmdashNano Research 311 779-793 (2010)13 Donkor A D et al Carbon nanotubes inhibit the hemolytic activity of the pore-forming toxin pyolysinmdashNano Research 27 517-525 (2009)14 Kam N W S et al Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destructionmdash proceedings of the National Academy of Sciences 10233 11600-11605 (2005)15 Robinson J T et al In Vivo Fluorescence Imaging in the Second Near- Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor UptakemdashJournal of the American Chemical Society 13425 10664-10669 (2012)16 Ghafari p Impact of single-walled carbon nanotubes on ciliated protozoa amp bacteriamdashMasterrsquos Thesis University of Waterloo (2009)17 Grabinski C et al Effect of particle dimension on biocompatibility of carbon nanomaterialsmdashCarbon 4514 2828-2835 (2007)

ConclusionDiscussion

Aggregated nanoparticles pose a difficult problem in nano-biomedicine1011 In this study the toxicity of As- Made SWCNTs (which contained visible aggregates) was examined however this data is representative of most nanoparticles The SWCNTs were separated into two groupsmdashone group was As-Made without any purification step to remove aggregates while the second group underwent ultracentrifugation in the Beckman Coulter Optima MAX-Xp ultracentrifuge While centrifugation procedures have been shown to effectively remove aggregated nanoparticles and are a standard for purification of SWCNTs the long centrifugation times (six hours or more) at low speeds (5000 x g to 22000 x g)12-16 are a hindrance to research workflow Our new ultracentrifugation method demonstrates that a two-minute high-speed ultracentrifugation achieves the same biocompatibility and individual solubilized SWCNTs15 as the longer centrifugation timemdasha 180-fold time savings to researchers

Optical images and dynamic light scattering data taken using the DelsaMax pRO are evidence that all aggregated SWCNT have been removed by the rapid ultracentrifugation The toxicity data gathered in this study was possible due to the use of Vi-CELL XR the strong absorption of the aggregates would confound typical MTT and MMp toxicity assays The Vi-CELL XR was programmed to specifically look for spherical cells with defined outlines in a sharply delineated size range ensuring that counting of carbon nanotube aggregates as either viable or dead cells was minimized The Vi-CELL XR optimization is important because of the large number of aggregates present even after cell washing As a control ultracentrifuged nanotubes were run without cells in this trial the Vi-CELL XR did not count a single live or dead cell Aggregates show increased toxicity over ultracentrifuged SWCNT which can be attributed to poor surfactant coverage and larger size of aggregated SWCNT Aggregated SWCNT have more exposed surfactant-free surface this increased surface availability of SWCNT directly contributes to an increase in reactive oxygen species (ROS)17 Furthermore aggregated or As-Made SWCNTs are much larger on a whole as demonstrated by the dynamic light scattering data the increased SWCNT size can block cell-signaling pathways or disrupt cellular action inhibiting cell growth17 Thus it is impor tant that aggregated nanoparticles are removed before being used in vitro or in vivo10

Industry 97 9

C e n t r i f u g a t i o n

wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

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Utilisation avec la centrifugeuse Avanti J-26S Xp et J-HC

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Mesure du taux drsquooxygegravene et de la permeacuteabiliteacute de lrsquoemballage

Le transmetteur Fibox 4 trace est lrsquooutil ideacuteal pour les tests dans lrsquoindustrie du packaging et la recherche et le controcircle de qualiteacute des mateacuteriaux La manipulation aiseacutee du systegraveme et la vitesse rapide drsquoeacutetalonnage permettent drsquoeffectuer dans un deacutelai record la mesure de la peacuteneacutetration de loxygegravene et des taux de transmission dans des centaines de contenants et drsquoemballages sans les endommager Un seul scanning du code-barres est

suffisant pour deacutemarrer une mesure preacutecise de traces drsquooxygegravene En combinaison avec le deacutetecteur de type pSt9 qui permet des mesures allant jusquagrave 05 ppm doxygegravene le capteur Fibox permet de deacuteterminer des taux de concentration doxygegravene pour des applications tregraves sensibles ou par exemple pour des emballages conccedilus en mateacuteriaux tregraves denses qui constituent une barriegravere eacuteleveacutee - comme les panneaux disolation sous vide ou de modules de panneaux photovoltaiumlques

Drsquoautres domaines drsquoapplications

- packaging et recherche de mateacuteriaux - QC- Controcircle de qualiteacute en agro-alimentaire brasserie- Recherche en biologie- Recherche environmentalhellip- proceacutedeacutes de bioproduction

NeW Presens Fibox 4 amp Fibox 4 trace Capteur drsquooxygegravene agrave transmission par fibre optique non invasif et autonome

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NEW Catalogue 2014 gratuit disponible sur demande cpnanalisbe et teacuteleacutechargeable sur wwanalisbePREsENsCATALOg

Industry 97 11

o 2 - C o 2 - p H D e t e c t i o n

wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeDELSAPRotEiN

Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

Industry 9712

P a r t i c l e C h a r a c t e r i z a t i o n

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wwwanalisbeDELSAPRotEiN

Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

Industry 97 13

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wwwanalisbeDELSAPRotEiN

Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

P a r t i c l e C h a r a c t e r i z a t i o n

wwwanalisbeInfo Ken De Roover I Tel 09 243 77 10 I flashanalisbe

ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

Industry 9716wwwanalisbe

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M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

Industry 97 17wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

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Industry 9718wwwanalisbe

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

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wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

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Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

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Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

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sansrisqueauxUV

Industry 97 27

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

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L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

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strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

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Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

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TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

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RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

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Industry 9730wwwanalisbe

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P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

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Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

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Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

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Industry 9732wwwanalisbe

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Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

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P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

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v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

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720

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CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

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T h e r m o s t a t i s a t i o n

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NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

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toP tECH F12-MA (ref 9153612)

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PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

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objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

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bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

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Page 4: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

SommaireCentrifugation07 Particle Characterization amp Centrifugation - Nanotoxicity I beckman coulter

10 Beckman Coulter Avanti J-26S XP I beckman coulterLa centrifugeuse Avanti J-26S permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils

Particle Characterization12 Particle Characterization - Nanotube I beckman coulter

Elemental analysis15 Short Application HR-CS AAS - Graphite furnace I analytik Jena

Determination of Se Sb Pb and Sn in natural water and mud samples

Microwave Sample Prep16 Single Reaction Chamber (SRC) I milestone

Digestion in a benchtop package

Lab Reactor - Synthesis18 NEW Reactor-Ready Pilot in detail I radleys

It is the attention to detail and wealth of innovative features that makes Reactor-Ready Pilot so much better

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Capillary Electrophoresis23 Analysis of Casein and Whey Protein in Whole 2 and Skim Milk I beckman coulter

Liquid Handling28 Analis - Pipette Calibration Service

Depuis plus de 20 ans Analis propose un service de calibration de pipettes

29 Artel PCSreg - Comment ameacuteliorer la qualiteacute de vos manipulations de liquide I artelComment obtenir des reacutesultats toujours preacutecis reproductibles et traccedilables selon les normes NIST

Quality Control amp Packaging Testing30 texte intro I techlab - rdm - presens - lloyd - chatillon - karg

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Freeze Drying42 Pourquoi choisir un lyophilisateur MArtIN CHrIst I MARTIN CHRIST

Thermostatisation44 NeW La nouvelle geacuteneacuteration drsquoincubateurs et de fours I MEMMERT

46 Materials testing at a high level I BINDER

48 simulation environnementale I BINDERSeacutechage amp chauffage

Product view New amp Promo06 Microcentrifugeuse NeW

BECKMAN COULTER I Seacuterie 20

11 Capteur drsquooxygegravene NeWpRESENS I Fibox 4 amp Fibox 4 trace

17 Microwave digestion MILESTONE I UltraWave

22 Capillary electrophoresis BECKMAN COULTER I pA800plus

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52 Cryostat JULABO ProMo

53 systegraveme de reacutegulation de tempeacuterature NeWJULABO I presto W91 et W92

53 systegraveme de reacutegulation de tempeacuteratureJULABO I presto W80

54 Laveurs-deacutesinfecteurs MIELE CoNCoUrs

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Beckman Coulter Application NoteParticle Characterization amp Centrifugation Nanotoxicity

Abstract

Nanomedicine is a field common to materials scientists chemists biomedical engineers biologists and medical scientists The following application note will help researchers in this field gain insight into the inherent shortcomings that are present in traditional cellular toxicity assays and how these shortcomings are magnified when the toxicity assay involves nanomaterial Researchers will also benefit from understanding how Beckman Coulter centrifugation and particle characterization instruments can significantly improve the accuracy and workflow in both preparing nanoparticles and assessing the nanoparticles in vitro toxicity

Introduction

Nanotechnology biomedicine is an emerging field still in its nascent phase Nanoparticles including quantum dots (QDs) carbon nanotubes (CNTs) and graphene have many unique spectral features that have specific application for in vivo imaging and drug delivery1 QDs23 CNTs1 and graphene1 all fluoresce in the advantageous biological window allowing for deeper imaging with better sensitivity45 the strongly red-shifted Raman signal of CNTs has also been exploited for in vivo imaging6 High surface area and strong light absorption of CNTs and graphene also make them excellent drug delivery and photothermal therapy agents1 However toxicity characterization of biologically focused nanomaterial poses a challenge to researchers because of these unique properties

Figure 1 Images of SWCNT Optical images of single-walled carbon nanotube (a) without centrifugation and (b) with ultracentrifugation for two minutes at 55000 RpM (~131000 x g) Note the presence of black aggregated SWCNT in the sample that was not ultracentrifuged

The intrinsic fluorescence and absorption of each of these nanomaterials overlap with the absorption and fluorescence region used typically in vitro cell toxicity assays leading to inaccurate and misleading results7-9 Even more confounding is the fact that absorption fluorescence and toxicity1011 properties of these materials are greatly altered when they are aggregated together compared with individually solubilized thus toxicity studies comparing aggregated vs non-aggregated have systemic bias Aggregated nanoparticles are typically removed by long centrifugation processes ie CNTs typically undergo six-hour centrifugations at 22000 x g to pellet aggregates12-16 This application note highlights how the Beckman Coulter Optima MAX-Xp ultracentrifuge was used to effectively reduce the time to remove the aggregated nanotubes while the Vi-CELL XR from Beckman Coulter quantified cell toxicity in the presence of single-walled carbon nanotubes (SWCNT)

Carbon Nanotube Preparation

Single-walled carbon nanotubes (Sigma-Aldrich) were mixed with 02 1 2-Distearoyl-phospha tidylethanolamine-methyl-polyethyleneglycol(DSpE-mpEG 5 kDa molecular weight Laysan Bio) in 10 mL of water The solution was bath-sonicated for 30 minutes to create well-dispersed carbon nanotubes following previously established procedures Using a TLA-1202 rotor in an Optima MAX-Xp Ultra-centrifuge 5 mL of SWCNT solution was centrifuged in open-top polycarbonate centri-fuge tubes (Beckman Coulter pN 343778) at 22degC 55000 RpM (~131000 x g) for two minutes The top 650 μL of supernatant was collected with care to avoid disturbing the pelleted aggregates The ultracentrifuged SWCNT (referred to as UCFrsquod SWCNT) and the remaining uncentrifuged SWCNT (referred to as As-Made SWCNT) were concentrated using 10 kDa Amicon Ultra 05 mL Centri- fugal Filters (Millipore) with a Beckman Coulter Microfuge 20 microcentrifuge The concen-tration was quantified using a UV-Vis-NIR spectrophotometer (paradigm Molecular Devices) and the established mass extinction coefficient of SWCNTs at 808 nm of 465 Lgcm12 After concentration UCFrsquod SWCNTs and As-Made SWCNTs were dilu-ted using deionized water to concentrations of 06 mgmL 03 mgmL and 006 mgmL

Beckman Coulter Optima Max-Xp Ultracentrifuge

Beckman Coulter Microfuge 20 Microcentrifuge

Industry 97 7wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

C e n t r i f u g a t i o n

Toxicity Assay

MCF-7 breast cancer cells were plated at a density of 008 x 106 per well in a 24-well plate with 900 μL of RPMI10 FBS (Invitrogen) 24 hours before the addition of nanotubes Cell viability and growth were confirmed using one of the wells before the addition of nanotubes 100 μL of SWCNT samples were added to wells on the second day There were six SWCNT groups (n=2group) in total 006 mgmL UCFrsquod SWCNTs 006 mgmL As- Made SWCNTs 003 mgmL UCFrsquod SWCNTs 003 mgmL As-Made SWCNTs 0006 mgmL UCFrsquod SWCNTs and 0006 mgmL As-Made SWCNTs 100 μL of DSPE-mPEG only sample was added to wells serving as a control There were three surfactant buffer control groups (n=2group) in total 02 mgmL DSpE-mpEG 002 mgmL DSpE-mpEG and 0002 mgmL DSpE-mpEG Finally control 1 (n=1) was a complete control with cells left untouched and control 2 (n=1) had 100 μL of sterilized water added After 24 hours all the wells were washed with pBS trypsinized and mixed in 1 mL of pBS for counting in the Vi-CELL XR A new cell type was created in the Vi-CELL XR software to minimize the counting of aggregated nanotubes as cells percentages of viable cells were used to compare cell viability and difference in the two solutions

Figure 2 Cell Imaging MCF-7 cells were imaged under an optical microscope after 24 hours of incubation with SWCNT The cells incubated with either 006 mgmL As-Made SWNT (left image) or 006 mgmL ultracentrifuged SWNT (right image) have not yet reached confluence Black aggregates of SWNT can be seen in the image on the left these aggregates are difficult to wash away without washing away the cells as well The aggregates have absorption and fluorescence properties that will skew traditional toxicity assays

Figure 3 Viability Results At all concentrations ultracentrifuged SWCNT (designated by UC) had minimal toxicity 75 or more of the MCF-7 cells remained viable 24 hours after incubation Contrastingly SWCNT that were not centrifuged and contained aggregated species (designated by AG) had increasing toxicity toward MCF-7 cells that scaled with increasing concentration At a stock concentration of 06 mgmL corresponding to a concentration in solution with cells at 006 mgmL the aggregated SWCNTs had greater than 50 cell death

Figure 4 Size Distribution Data Single-walled carbon nanotubes after sonication in surfactant still have a number of aggregated species Size distribution determined by Dynamic Light Scattering on the DelsaMax pRO showed two broad species (red line) The first size range roughly 100 nm in diameter represents individually solubilized carbon nanotubes The second species containing mostly aggregated carbon nanotubes has a diameter peak closer to one micron in size After a two-minute ultracentrifugation the SWNT demonstrate only a single broad species at 100 nm indicating that virtually all aggregates have been removed This is further indicated by a 59 decrease in polydispersity Interestingly the zeta potential remains unchanged between aggregated and centrifuged carbon nanotubes This is most likely due to the fact that steric repulsion from the poly(ethylene glycol) surfactant provides most of the stability to the carbon nanotubes while electrostatic repulsion does not play a major role

Beckman Coulter Vi-CELL XR Cell Viability Analyser

Beckman Coulter DelsaMax pROparticle Characterization

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Reacutefeacuterences

1 Liu Z et al Carbon materials for drug delivery amp cancer therapymdashMaterials Today 147 316-323 (2011)2 Hong G et al In Vivo Fluorescence Imaging with Ag2S Quantum Dots in the Second Near-Infrared RegionmdashAngewandte Chemie 12439 9956-9959 (2012)3 Levina L et al Efficient Infrared-Emitting PbS Quantum Dots Grown on DNA and Stable in Aqueous Solution and Blood plasmamdashAdvanced Materials 1715 1854-1857 (2005)4 Welsher K Sherlock S p and Dai H Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared windowmdashproceedings of the National Academy of Sciences 10822 8943-8948 (2011)5 Smith A M Mancini M C and Nie S Second window for in vivo imagingmdash Nature Nanotechnology 411 710 (2009)6 Zavaleta C et al Noninvasive Raman spectroscopy in living mice for evaluation of tumor targeting with carbon nanotubesmdashNano letters 89 2800-2805 (2008)7 Davoren M et al In vitro toxicity evaluation of single-walled carbon nanotubes on human A549 lung cellsmdashToxicology in vitro an international journal published in association with BIBRA 213 438 (2007)8 Woumlrle-Knirsch J M pulskamp K and Krug H F Oops they did it again Carbon nanotubes hoax scientists in viability assaysmdashNano letters 66 1261-1268 (2006)9 Ren H et al Toxicity of single-walled carbon nanotube How we were wrongmdashMaterials Today 131 6-8 (2010)

10 Wick p et al The degree and kind of agglomeration affect carbon nanotube cytotoxicitymdashToxicology letters 1682 121-131 (2007)11 Lewinski N Colvin V and Drezek R Cytotoxicity of nanoparticlesmdashSmall 41 26-49 (2008)12 Robinson J T et al High performance in vivo near-IR (gt 1 μm) imaging and photothermal cancer therapy with carbon nanotubesmdashNano Research 311 779-793 (2010)13 Donkor A D et al Carbon nanotubes inhibit the hemolytic activity of the pore-forming toxin pyolysinmdashNano Research 27 517-525 (2009)14 Kam N W S et al Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destructionmdash proceedings of the National Academy of Sciences 10233 11600-11605 (2005)15 Robinson J T et al In Vivo Fluorescence Imaging in the Second Near- Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor UptakemdashJournal of the American Chemical Society 13425 10664-10669 (2012)16 Ghafari p Impact of single-walled carbon nanotubes on ciliated protozoa amp bacteriamdashMasterrsquos Thesis University of Waterloo (2009)17 Grabinski C et al Effect of particle dimension on biocompatibility of carbon nanomaterialsmdashCarbon 4514 2828-2835 (2007)

ConclusionDiscussion

Aggregated nanoparticles pose a difficult problem in nano-biomedicine1011 In this study the toxicity of As- Made SWCNTs (which contained visible aggregates) was examined however this data is representative of most nanoparticles The SWCNTs were separated into two groupsmdashone group was As-Made without any purification step to remove aggregates while the second group underwent ultracentrifugation in the Beckman Coulter Optima MAX-Xp ultracentrifuge While centrifugation procedures have been shown to effectively remove aggregated nanoparticles and are a standard for purification of SWCNTs the long centrifugation times (six hours or more) at low speeds (5000 x g to 22000 x g)12-16 are a hindrance to research workflow Our new ultracentrifugation method demonstrates that a two-minute high-speed ultracentrifugation achieves the same biocompatibility and individual solubilized SWCNTs15 as the longer centrifugation timemdasha 180-fold time savings to researchers

Optical images and dynamic light scattering data taken using the DelsaMax pRO are evidence that all aggregated SWCNT have been removed by the rapid ultracentrifugation The toxicity data gathered in this study was possible due to the use of Vi-CELL XR the strong absorption of the aggregates would confound typical MTT and MMp toxicity assays The Vi-CELL XR was programmed to specifically look for spherical cells with defined outlines in a sharply delineated size range ensuring that counting of carbon nanotube aggregates as either viable or dead cells was minimized The Vi-CELL XR optimization is important because of the large number of aggregates present even after cell washing As a control ultracentrifuged nanotubes were run without cells in this trial the Vi-CELL XR did not count a single live or dead cell Aggregates show increased toxicity over ultracentrifuged SWCNT which can be attributed to poor surfactant coverage and larger size of aggregated SWCNT Aggregated SWCNT have more exposed surfactant-free surface this increased surface availability of SWCNT directly contributes to an increase in reactive oxygen species (ROS)17 Furthermore aggregated or As-Made SWCNTs are much larger on a whole as demonstrated by the dynamic light scattering data the increased SWCNT size can block cell-signaling pathways or disrupt cellular action inhibiting cell growth17 Thus it is impor tant that aggregated nanoparticles are removed before being used in vitro or in vivo10

Industry 97 9

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NeW Beckman Coulter Avanti J-26s La centrifugeuse Avanti J-26s permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils

La meilleure dans sa cateacutegorieLa centrifugeuse Avanti J-26S Xp permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils Un seul systegraveme traite 6 litres de produit de fermentation en moins de 10 minutes fournit une fraction subcellulaire agrave 82 000 x g centrifuge 24 plaques de microtitration en une fois et seacutepare des populations de cellules vivantes par eacutelutriation

Fonctionnement rapideGracircce agrave la technologie de switched reluctance (SR) drive agrave couple de deacutemarrage eacuteleveacute le temps de cycle est raccourci et il ne faut plus attendre lrsquoeacutechantillon en fin de run En outre le systegraveme de reacuteduction des frictions assure un fonctionnement plus rapide plus silencieux agrave tempeacuterature plus basse et avec une consommation drsquoeacutenergie plus basse que pour les centrifugeuses classiques

Fonctionnement seacutecuriseacuteplusieurs niveaux de bioseacutecuriteacute inteacutegreacutes au niveau des contenants (couvercles joints eacutevents) des rotors (canisters et couvercles seacutecuriseacutes) et de la centrifugeuse (filtres HEPA) A cette bioseacutecuriteacute srsquoajoute lrsquoidentification automatique des rotors pour offrir plus de seacutecuriteacute aux utilisateurs

Toujours precirct agrave lrsquoemploiMecircme si vous nrsquoutilisez pas immeacutediatement tout son potentiel la centrifugeuse Avanti J-26S Xp sera toujours precircte pour reacutepondre aux changements de besoins dans vos recherches Lrsquoassortiment de 26 rotors (parmi lesquels des rotors plus leacutegers des rotors pour centrifuger en continu et eacutelutrier ainsi qursquoune gamme complegravete de rotors BioSafe) fait que lrsquoAvanti J-26S Xp sera sans doute la seule centrifugeuse agrave haute performance dont votre laboratoire aura jamais besoin

Rotor JLA-81000 agrave angle fixe

Utilisation avec la centrifugeuse Avanti J-26S Xp et J-HC

Le systegraveme de rotor composite JLA-81000 avec des pochettes auto-scellan-tes Harvestline traite six litres complets en moins de 10 minutes Sa construc-tion leacutegegravere et les canisters amovibles le rendent tregraves commode pour le traite-ment de gros volumes Les bouteilles J-LITE et leurs couvercles de fermeture sont ideacuteaux pour les reacutecoltes bacteacuteriennes et de cultures cellulaires au deacutepart de fermenteurs pour lrsquoextraction de deacutebris cellulaires pour la purification de gros volumes de virus et polysaccharides dans le cadre de la production de vaccins ou pour la concentration de boues et la clarification de surnageants Ce rotor deacuteveloppe jusqursquoagrave 15 900 x g pour de hauts rendements horaires En permettant des seacuteparations les plus efficaces avec une capaciteacute totale de 6 litres le JLA-81000 peut traiter 36 litres par heure et est de ce fait le plus efficace des rotors disponibles sur le marcheacute

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Mesure du taux drsquooxygegravene et de la permeacuteabiliteacute de lrsquoemballage

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suffisant pour deacutemarrer une mesure preacutecise de traces drsquooxygegravene En combinaison avec le deacutetecteur de type pSt9 qui permet des mesures allant jusquagrave 05 ppm doxygegravene le capteur Fibox permet de deacuteterminer des taux de concentration doxygegravene pour des applications tregraves sensibles ou par exemple pour des emballages conccedilus en mateacuteriaux tregraves denses qui constituent une barriegravere eacuteleveacutee - comme les panneaux disolation sous vide ou de modules de panneaux photovoltaiumlques

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Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

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Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

Industry 97 13

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wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

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Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

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ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

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M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

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M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

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Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

entiegraverementautoclavables(121degC100kPa20min)etpeuventecirctresteacuteriliseacutees

sansrisqueauxUV

Industry 97 27

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

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Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

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Beckman Coulter Application NoteParticle Characterization amp Centrifugation Nanotoxicity

Abstract

Nanomedicine is a field common to materials scientists chemists biomedical engineers biologists and medical scientists The following application note will help researchers in this field gain insight into the inherent shortcomings that are present in traditional cellular toxicity assays and how these shortcomings are magnified when the toxicity assay involves nanomaterial Researchers will also benefit from understanding how Beckman Coulter centrifugation and particle characterization instruments can significantly improve the accuracy and workflow in both preparing nanoparticles and assessing the nanoparticles in vitro toxicity

Introduction

Nanotechnology biomedicine is an emerging field still in its nascent phase Nanoparticles including quantum dots (QDs) carbon nanotubes (CNTs) and graphene have many unique spectral features that have specific application for in vivo imaging and drug delivery1 QDs23 CNTs1 and graphene1 all fluoresce in the advantageous biological window allowing for deeper imaging with better sensitivity45 the strongly red-shifted Raman signal of CNTs has also been exploited for in vivo imaging6 High surface area and strong light absorption of CNTs and graphene also make them excellent drug delivery and photothermal therapy agents1 However toxicity characterization of biologically focused nanomaterial poses a challenge to researchers because of these unique properties

Figure 1 Images of SWCNT Optical images of single-walled carbon nanotube (a) without centrifugation and (b) with ultracentrifugation for two minutes at 55000 RpM (~131000 x g) Note the presence of black aggregated SWCNT in the sample that was not ultracentrifuged

The intrinsic fluorescence and absorption of each of these nanomaterials overlap with the absorption and fluorescence region used typically in vitro cell toxicity assays leading to inaccurate and misleading results7-9 Even more confounding is the fact that absorption fluorescence and toxicity1011 properties of these materials are greatly altered when they are aggregated together compared with individually solubilized thus toxicity studies comparing aggregated vs non-aggregated have systemic bias Aggregated nanoparticles are typically removed by long centrifugation processes ie CNTs typically undergo six-hour centrifugations at 22000 x g to pellet aggregates12-16 This application note highlights how the Beckman Coulter Optima MAX-Xp ultracentrifuge was used to effectively reduce the time to remove the aggregated nanotubes while the Vi-CELL XR from Beckman Coulter quantified cell toxicity in the presence of single-walled carbon nanotubes (SWCNT)

Carbon Nanotube Preparation

Single-walled carbon nanotubes (Sigma-Aldrich) were mixed with 02 1 2-Distearoyl-phospha tidylethanolamine-methyl-polyethyleneglycol(DSpE-mpEG 5 kDa molecular weight Laysan Bio) in 10 mL of water The solution was bath-sonicated for 30 minutes to create well-dispersed carbon nanotubes following previously established procedures Using a TLA-1202 rotor in an Optima MAX-Xp Ultra-centrifuge 5 mL of SWCNT solution was centrifuged in open-top polycarbonate centri-fuge tubes (Beckman Coulter pN 343778) at 22degC 55000 RpM (~131000 x g) for two minutes The top 650 μL of supernatant was collected with care to avoid disturbing the pelleted aggregates The ultracentrifuged SWCNT (referred to as UCFrsquod SWCNT) and the remaining uncentrifuged SWCNT (referred to as As-Made SWCNT) were concentrated using 10 kDa Amicon Ultra 05 mL Centri- fugal Filters (Millipore) with a Beckman Coulter Microfuge 20 microcentrifuge The concen-tration was quantified using a UV-Vis-NIR spectrophotometer (paradigm Molecular Devices) and the established mass extinction coefficient of SWCNTs at 808 nm of 465 Lgcm12 After concentration UCFrsquod SWCNTs and As-Made SWCNTs were dilu-ted using deionized water to concentrations of 06 mgmL 03 mgmL and 006 mgmL

Beckman Coulter Optima Max-Xp Ultracentrifuge

Beckman Coulter Microfuge 20 Microcentrifuge

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C e n t r i f u g a t i o n

Toxicity Assay

MCF-7 breast cancer cells were plated at a density of 008 x 106 per well in a 24-well plate with 900 μL of RPMI10 FBS (Invitrogen) 24 hours before the addition of nanotubes Cell viability and growth were confirmed using one of the wells before the addition of nanotubes 100 μL of SWCNT samples were added to wells on the second day There were six SWCNT groups (n=2group) in total 006 mgmL UCFrsquod SWCNTs 006 mgmL As- Made SWCNTs 003 mgmL UCFrsquod SWCNTs 003 mgmL As-Made SWCNTs 0006 mgmL UCFrsquod SWCNTs and 0006 mgmL As-Made SWCNTs 100 μL of DSPE-mPEG only sample was added to wells serving as a control There were three surfactant buffer control groups (n=2group) in total 02 mgmL DSpE-mpEG 002 mgmL DSpE-mpEG and 0002 mgmL DSpE-mpEG Finally control 1 (n=1) was a complete control with cells left untouched and control 2 (n=1) had 100 μL of sterilized water added After 24 hours all the wells were washed with pBS trypsinized and mixed in 1 mL of pBS for counting in the Vi-CELL XR A new cell type was created in the Vi-CELL XR software to minimize the counting of aggregated nanotubes as cells percentages of viable cells were used to compare cell viability and difference in the two solutions

Figure 2 Cell Imaging MCF-7 cells were imaged under an optical microscope after 24 hours of incubation with SWCNT The cells incubated with either 006 mgmL As-Made SWNT (left image) or 006 mgmL ultracentrifuged SWNT (right image) have not yet reached confluence Black aggregates of SWNT can be seen in the image on the left these aggregates are difficult to wash away without washing away the cells as well The aggregates have absorption and fluorescence properties that will skew traditional toxicity assays

Figure 3 Viability Results At all concentrations ultracentrifuged SWCNT (designated by UC) had minimal toxicity 75 or more of the MCF-7 cells remained viable 24 hours after incubation Contrastingly SWCNT that were not centrifuged and contained aggregated species (designated by AG) had increasing toxicity toward MCF-7 cells that scaled with increasing concentration At a stock concentration of 06 mgmL corresponding to a concentration in solution with cells at 006 mgmL the aggregated SWCNTs had greater than 50 cell death

Figure 4 Size Distribution Data Single-walled carbon nanotubes after sonication in surfactant still have a number of aggregated species Size distribution determined by Dynamic Light Scattering on the DelsaMax pRO showed two broad species (red line) The first size range roughly 100 nm in diameter represents individually solubilized carbon nanotubes The second species containing mostly aggregated carbon nanotubes has a diameter peak closer to one micron in size After a two-minute ultracentrifugation the SWNT demonstrate only a single broad species at 100 nm indicating that virtually all aggregates have been removed This is further indicated by a 59 decrease in polydispersity Interestingly the zeta potential remains unchanged between aggregated and centrifuged carbon nanotubes This is most likely due to the fact that steric repulsion from the poly(ethylene glycol) surfactant provides most of the stability to the carbon nanotubes while electrostatic repulsion does not play a major role

Beckman Coulter Vi-CELL XR Cell Viability Analyser

Beckman Coulter DelsaMax pROparticle Characterization

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Reacutefeacuterences

1 Liu Z et al Carbon materials for drug delivery amp cancer therapymdashMaterials Today 147 316-323 (2011)2 Hong G et al In Vivo Fluorescence Imaging with Ag2S Quantum Dots in the Second Near-Infrared RegionmdashAngewandte Chemie 12439 9956-9959 (2012)3 Levina L et al Efficient Infrared-Emitting PbS Quantum Dots Grown on DNA and Stable in Aqueous Solution and Blood plasmamdashAdvanced Materials 1715 1854-1857 (2005)4 Welsher K Sherlock S p and Dai H Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared windowmdashproceedings of the National Academy of Sciences 10822 8943-8948 (2011)5 Smith A M Mancini M C and Nie S Second window for in vivo imagingmdash Nature Nanotechnology 411 710 (2009)6 Zavaleta C et al Noninvasive Raman spectroscopy in living mice for evaluation of tumor targeting with carbon nanotubesmdashNano letters 89 2800-2805 (2008)7 Davoren M et al In vitro toxicity evaluation of single-walled carbon nanotubes on human A549 lung cellsmdashToxicology in vitro an international journal published in association with BIBRA 213 438 (2007)8 Woumlrle-Knirsch J M pulskamp K and Krug H F Oops they did it again Carbon nanotubes hoax scientists in viability assaysmdashNano letters 66 1261-1268 (2006)9 Ren H et al Toxicity of single-walled carbon nanotube How we were wrongmdashMaterials Today 131 6-8 (2010)

10 Wick p et al The degree and kind of agglomeration affect carbon nanotube cytotoxicitymdashToxicology letters 1682 121-131 (2007)11 Lewinski N Colvin V and Drezek R Cytotoxicity of nanoparticlesmdashSmall 41 26-49 (2008)12 Robinson J T et al High performance in vivo near-IR (gt 1 μm) imaging and photothermal cancer therapy with carbon nanotubesmdashNano Research 311 779-793 (2010)13 Donkor A D et al Carbon nanotubes inhibit the hemolytic activity of the pore-forming toxin pyolysinmdashNano Research 27 517-525 (2009)14 Kam N W S et al Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destructionmdash proceedings of the National Academy of Sciences 10233 11600-11605 (2005)15 Robinson J T et al In Vivo Fluorescence Imaging in the Second Near- Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor UptakemdashJournal of the American Chemical Society 13425 10664-10669 (2012)16 Ghafari p Impact of single-walled carbon nanotubes on ciliated protozoa amp bacteriamdashMasterrsquos Thesis University of Waterloo (2009)17 Grabinski C et al Effect of particle dimension on biocompatibility of carbon nanomaterialsmdashCarbon 4514 2828-2835 (2007)

ConclusionDiscussion

Aggregated nanoparticles pose a difficult problem in nano-biomedicine1011 In this study the toxicity of As- Made SWCNTs (which contained visible aggregates) was examined however this data is representative of most nanoparticles The SWCNTs were separated into two groupsmdashone group was As-Made without any purification step to remove aggregates while the second group underwent ultracentrifugation in the Beckman Coulter Optima MAX-Xp ultracentrifuge While centrifugation procedures have been shown to effectively remove aggregated nanoparticles and are a standard for purification of SWCNTs the long centrifugation times (six hours or more) at low speeds (5000 x g to 22000 x g)12-16 are a hindrance to research workflow Our new ultracentrifugation method demonstrates that a two-minute high-speed ultracentrifugation achieves the same biocompatibility and individual solubilized SWCNTs15 as the longer centrifugation timemdasha 180-fold time savings to researchers

Optical images and dynamic light scattering data taken using the DelsaMax pRO are evidence that all aggregated SWCNT have been removed by the rapid ultracentrifugation The toxicity data gathered in this study was possible due to the use of Vi-CELL XR the strong absorption of the aggregates would confound typical MTT and MMp toxicity assays The Vi-CELL XR was programmed to specifically look for spherical cells with defined outlines in a sharply delineated size range ensuring that counting of carbon nanotube aggregates as either viable or dead cells was minimized The Vi-CELL XR optimization is important because of the large number of aggregates present even after cell washing As a control ultracentrifuged nanotubes were run without cells in this trial the Vi-CELL XR did not count a single live or dead cell Aggregates show increased toxicity over ultracentrifuged SWCNT which can be attributed to poor surfactant coverage and larger size of aggregated SWCNT Aggregated SWCNT have more exposed surfactant-free surface this increased surface availability of SWCNT directly contributes to an increase in reactive oxygen species (ROS)17 Furthermore aggregated or As-Made SWCNTs are much larger on a whole as demonstrated by the dynamic light scattering data the increased SWCNT size can block cell-signaling pathways or disrupt cellular action inhibiting cell growth17 Thus it is impor tant that aggregated nanoparticles are removed before being used in vitro or in vivo10

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Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

Industry 9712

P a r t i c l e C h a r a c t e r i z a t i o n

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wwwanalisbeDELSAPRotEiN

Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

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wwwanalisbeDELSAPRotEiN

Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

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ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

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single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

Industry 9716wwwanalisbe

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M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

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M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

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L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

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Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

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Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

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Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

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Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

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Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

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Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

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Industry 97 27

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wwwanalisbeInfo Maria Fraile I Teacutel 09 243 77 12 I mfeanalisbe

ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

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forfaitaire tregraves compeacutetitif- oPtioN inout

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votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

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reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

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strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

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Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

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Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

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Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

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Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

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wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

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wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

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Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

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Alar

me

de

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peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

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agrave vi

tess

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Porte

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objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

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Beckman Coulter Application NoteParticle Characterization amp Centrifugation Nanotoxicity

Abstract

Nanomedicine is a field common to materials scientists chemists biomedical engineers biologists and medical scientists The following application note will help researchers in this field gain insight into the inherent shortcomings that are present in traditional cellular toxicity assays and how these shortcomings are magnified when the toxicity assay involves nanomaterial Researchers will also benefit from understanding how Beckman Coulter centrifugation and particle characterization instruments can significantly improve the accuracy and workflow in both preparing nanoparticles and assessing the nanoparticles in vitro toxicity

Introduction

Nanotechnology biomedicine is an emerging field still in its nascent phase Nanoparticles including quantum dots (QDs) carbon nanotubes (CNTs) and graphene have many unique spectral features that have specific application for in vivo imaging and drug delivery1 QDs23 CNTs1 and graphene1 all fluoresce in the advantageous biological window allowing for deeper imaging with better sensitivity45 the strongly red-shifted Raman signal of CNTs has also been exploited for in vivo imaging6 High surface area and strong light absorption of CNTs and graphene also make them excellent drug delivery and photothermal therapy agents1 However toxicity characterization of biologically focused nanomaterial poses a challenge to researchers because of these unique properties

Figure 1 Images of SWCNT Optical images of single-walled carbon nanotube (a) without centrifugation and (b) with ultracentrifugation for two minutes at 55000 RpM (~131000 x g) Note the presence of black aggregated SWCNT in the sample that was not ultracentrifuged

The intrinsic fluorescence and absorption of each of these nanomaterials overlap with the absorption and fluorescence region used typically in vitro cell toxicity assays leading to inaccurate and misleading results7-9 Even more confounding is the fact that absorption fluorescence and toxicity1011 properties of these materials are greatly altered when they are aggregated together compared with individually solubilized thus toxicity studies comparing aggregated vs non-aggregated have systemic bias Aggregated nanoparticles are typically removed by long centrifugation processes ie CNTs typically undergo six-hour centrifugations at 22000 x g to pellet aggregates12-16 This application note highlights how the Beckman Coulter Optima MAX-Xp ultracentrifuge was used to effectively reduce the time to remove the aggregated nanotubes while the Vi-CELL XR from Beckman Coulter quantified cell toxicity in the presence of single-walled carbon nanotubes (SWCNT)

Carbon Nanotube Preparation

Single-walled carbon nanotubes (Sigma-Aldrich) were mixed with 02 1 2-Distearoyl-phospha tidylethanolamine-methyl-polyethyleneglycol(DSpE-mpEG 5 kDa molecular weight Laysan Bio) in 10 mL of water The solution was bath-sonicated for 30 minutes to create well-dispersed carbon nanotubes following previously established procedures Using a TLA-1202 rotor in an Optima MAX-Xp Ultra-centrifuge 5 mL of SWCNT solution was centrifuged in open-top polycarbonate centri-fuge tubes (Beckman Coulter pN 343778) at 22degC 55000 RpM (~131000 x g) for two minutes The top 650 μL of supernatant was collected with care to avoid disturbing the pelleted aggregates The ultracentrifuged SWCNT (referred to as UCFrsquod SWCNT) and the remaining uncentrifuged SWCNT (referred to as As-Made SWCNT) were concentrated using 10 kDa Amicon Ultra 05 mL Centri- fugal Filters (Millipore) with a Beckman Coulter Microfuge 20 microcentrifuge The concen-tration was quantified using a UV-Vis-NIR spectrophotometer (paradigm Molecular Devices) and the established mass extinction coefficient of SWCNTs at 808 nm of 465 Lgcm12 After concentration UCFrsquod SWCNTs and As-Made SWCNTs were dilu-ted using deionized water to concentrations of 06 mgmL 03 mgmL and 006 mgmL

Beckman Coulter Optima Max-Xp Ultracentrifuge

Beckman Coulter Microfuge 20 Microcentrifuge

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C e n t r i f u g a t i o n

Toxicity Assay

MCF-7 breast cancer cells were plated at a density of 008 x 106 per well in a 24-well plate with 900 μL of RPMI10 FBS (Invitrogen) 24 hours before the addition of nanotubes Cell viability and growth were confirmed using one of the wells before the addition of nanotubes 100 μL of SWCNT samples were added to wells on the second day There were six SWCNT groups (n=2group) in total 006 mgmL UCFrsquod SWCNTs 006 mgmL As- Made SWCNTs 003 mgmL UCFrsquod SWCNTs 003 mgmL As-Made SWCNTs 0006 mgmL UCFrsquod SWCNTs and 0006 mgmL As-Made SWCNTs 100 μL of DSPE-mPEG only sample was added to wells serving as a control There were three surfactant buffer control groups (n=2group) in total 02 mgmL DSpE-mpEG 002 mgmL DSpE-mpEG and 0002 mgmL DSpE-mpEG Finally control 1 (n=1) was a complete control with cells left untouched and control 2 (n=1) had 100 μL of sterilized water added After 24 hours all the wells were washed with pBS trypsinized and mixed in 1 mL of pBS for counting in the Vi-CELL XR A new cell type was created in the Vi-CELL XR software to minimize the counting of aggregated nanotubes as cells percentages of viable cells were used to compare cell viability and difference in the two solutions

Figure 2 Cell Imaging MCF-7 cells were imaged under an optical microscope after 24 hours of incubation with SWCNT The cells incubated with either 006 mgmL As-Made SWNT (left image) or 006 mgmL ultracentrifuged SWNT (right image) have not yet reached confluence Black aggregates of SWNT can be seen in the image on the left these aggregates are difficult to wash away without washing away the cells as well The aggregates have absorption and fluorescence properties that will skew traditional toxicity assays

Figure 3 Viability Results At all concentrations ultracentrifuged SWCNT (designated by UC) had minimal toxicity 75 or more of the MCF-7 cells remained viable 24 hours after incubation Contrastingly SWCNT that were not centrifuged and contained aggregated species (designated by AG) had increasing toxicity toward MCF-7 cells that scaled with increasing concentration At a stock concentration of 06 mgmL corresponding to a concentration in solution with cells at 006 mgmL the aggregated SWCNTs had greater than 50 cell death

Figure 4 Size Distribution Data Single-walled carbon nanotubes after sonication in surfactant still have a number of aggregated species Size distribution determined by Dynamic Light Scattering on the DelsaMax pRO showed two broad species (red line) The first size range roughly 100 nm in diameter represents individually solubilized carbon nanotubes The second species containing mostly aggregated carbon nanotubes has a diameter peak closer to one micron in size After a two-minute ultracentrifugation the SWNT demonstrate only a single broad species at 100 nm indicating that virtually all aggregates have been removed This is further indicated by a 59 decrease in polydispersity Interestingly the zeta potential remains unchanged between aggregated and centrifuged carbon nanotubes This is most likely due to the fact that steric repulsion from the poly(ethylene glycol) surfactant provides most of the stability to the carbon nanotubes while electrostatic repulsion does not play a major role

Beckman Coulter Vi-CELL XR Cell Viability Analyser

Beckman Coulter DelsaMax pROparticle Characterization

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Reacutefeacuterences

1 Liu Z et al Carbon materials for drug delivery amp cancer therapymdashMaterials Today 147 316-323 (2011)2 Hong G et al In Vivo Fluorescence Imaging with Ag2S Quantum Dots in the Second Near-Infrared RegionmdashAngewandte Chemie 12439 9956-9959 (2012)3 Levina L et al Efficient Infrared-Emitting PbS Quantum Dots Grown on DNA and Stable in Aqueous Solution and Blood plasmamdashAdvanced Materials 1715 1854-1857 (2005)4 Welsher K Sherlock S p and Dai H Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared windowmdashproceedings of the National Academy of Sciences 10822 8943-8948 (2011)5 Smith A M Mancini M C and Nie S Second window for in vivo imagingmdash Nature Nanotechnology 411 710 (2009)6 Zavaleta C et al Noninvasive Raman spectroscopy in living mice for evaluation of tumor targeting with carbon nanotubesmdashNano letters 89 2800-2805 (2008)7 Davoren M et al In vitro toxicity evaluation of single-walled carbon nanotubes on human A549 lung cellsmdashToxicology in vitro an international journal published in association with BIBRA 213 438 (2007)8 Woumlrle-Knirsch J M pulskamp K and Krug H F Oops they did it again Carbon nanotubes hoax scientists in viability assaysmdashNano letters 66 1261-1268 (2006)9 Ren H et al Toxicity of single-walled carbon nanotube How we were wrongmdashMaterials Today 131 6-8 (2010)

10 Wick p et al The degree and kind of agglomeration affect carbon nanotube cytotoxicitymdashToxicology letters 1682 121-131 (2007)11 Lewinski N Colvin V and Drezek R Cytotoxicity of nanoparticlesmdashSmall 41 26-49 (2008)12 Robinson J T et al High performance in vivo near-IR (gt 1 μm) imaging and photothermal cancer therapy with carbon nanotubesmdashNano Research 311 779-793 (2010)13 Donkor A D et al Carbon nanotubes inhibit the hemolytic activity of the pore-forming toxin pyolysinmdashNano Research 27 517-525 (2009)14 Kam N W S et al Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destructionmdash proceedings of the National Academy of Sciences 10233 11600-11605 (2005)15 Robinson J T et al In Vivo Fluorescence Imaging in the Second Near- Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor UptakemdashJournal of the American Chemical Society 13425 10664-10669 (2012)16 Ghafari p Impact of single-walled carbon nanotubes on ciliated protozoa amp bacteriamdashMasterrsquos Thesis University of Waterloo (2009)17 Grabinski C et al Effect of particle dimension on biocompatibility of carbon nanomaterialsmdashCarbon 4514 2828-2835 (2007)

ConclusionDiscussion

Aggregated nanoparticles pose a difficult problem in nano-biomedicine1011 In this study the toxicity of As- Made SWCNTs (which contained visible aggregates) was examined however this data is representative of most nanoparticles The SWCNTs were separated into two groupsmdashone group was As-Made without any purification step to remove aggregates while the second group underwent ultracentrifugation in the Beckman Coulter Optima MAX-Xp ultracentrifuge While centrifugation procedures have been shown to effectively remove aggregated nanoparticles and are a standard for purification of SWCNTs the long centrifugation times (six hours or more) at low speeds (5000 x g to 22000 x g)12-16 are a hindrance to research workflow Our new ultracentrifugation method demonstrates that a two-minute high-speed ultracentrifugation achieves the same biocompatibility and individual solubilized SWCNTs15 as the longer centrifugation timemdasha 180-fold time savings to researchers

Optical images and dynamic light scattering data taken using the DelsaMax pRO are evidence that all aggregated SWCNT have been removed by the rapid ultracentrifugation The toxicity data gathered in this study was possible due to the use of Vi-CELL XR the strong absorption of the aggregates would confound typical MTT and MMp toxicity assays The Vi-CELL XR was programmed to specifically look for spherical cells with defined outlines in a sharply delineated size range ensuring that counting of carbon nanotube aggregates as either viable or dead cells was minimized The Vi-CELL XR optimization is important because of the large number of aggregates present even after cell washing As a control ultracentrifuged nanotubes were run without cells in this trial the Vi-CELL XR did not count a single live or dead cell Aggregates show increased toxicity over ultracentrifuged SWCNT which can be attributed to poor surfactant coverage and larger size of aggregated SWCNT Aggregated SWCNT have more exposed surfactant-free surface this increased surface availability of SWCNT directly contributes to an increase in reactive oxygen species (ROS)17 Furthermore aggregated or As-Made SWCNTs are much larger on a whole as demonstrated by the dynamic light scattering data the increased SWCNT size can block cell-signaling pathways or disrupt cellular action inhibiting cell growth17 Thus it is impor tant that aggregated nanoparticles are removed before being used in vitro or in vivo10

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Toujours precirct agrave lrsquoemploiMecircme si vous nrsquoutilisez pas immeacutediatement tout son potentiel la centrifugeuse Avanti J-26S Xp sera toujours precircte pour reacutepondre aux changements de besoins dans vos recherches Lrsquoassortiment de 26 rotors (parmi lesquels des rotors plus leacutegers des rotors pour centrifuger en continu et eacutelutrier ainsi qursquoune gamme complegravete de rotors BioSafe) fait que lrsquoAvanti J-26S Xp sera sans doute la seule centrifugeuse agrave haute performance dont votre laboratoire aura jamais besoin

Rotor JLA-81000 agrave angle fixe

Utilisation avec la centrifugeuse Avanti J-26S Xp et J-HC

Le systegraveme de rotor composite JLA-81000 avec des pochettes auto-scellan-tes Harvestline traite six litres complets en moins de 10 minutes Sa construc-tion leacutegegravere et les canisters amovibles le rendent tregraves commode pour le traite-ment de gros volumes Les bouteilles J-LITE et leurs couvercles de fermeture sont ideacuteaux pour les reacutecoltes bacteacuteriennes et de cultures cellulaires au deacutepart de fermenteurs pour lrsquoextraction de deacutebris cellulaires pour la purification de gros volumes de virus et polysaccharides dans le cadre de la production de vaccins ou pour la concentration de boues et la clarification de surnageants Ce rotor deacuteveloppe jusqursquoagrave 15 900 x g pour de hauts rendements horaires En permettant des seacuteparations les plus efficaces avec une capaciteacute totale de 6 litres le JLA-81000 peut traiter 36 litres par heure et est de ce fait le plus efficace des rotors disponibles sur le marcheacute

Pour plus dinformations au sujet de ces centrifugeuses et des configurations lieacutees agrave vos applications contactez nos speacutecialistes

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NEW wwwanalisbeoneliterbottle

Industry 9710

C e n t r i f u g a t i o n

wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

Capteur drsquooxygegravene agrave transmission par fibre optique non-invasif et autonome Le nouveau systegraveme de gestion de donneacutees et le lecteur code-barres acceacutelegravere le processus de mesure- Mesure sans aucun souci gracircce au systegraveme didentification

unique des senseurs

- Mesurer de 1 ppb agrave 100 doxygegravene avec un seul appareil

- Calibrage facile par scanning du code-barres

- Compensation de la tempeacuterature de la pression et la saliniteacute

- Tregraves bonne gestion de leacutenergie pour la mesure agrave long terme

- Le logiciel de gestion de la base de donneacutees et reacutesultats (en option) offre un controcircle simultaneacute de plusieurs appareils

Exemple drsquoapplication (drsquoautres applications sur notre site internet wwwanalisbeFIBOX)

Mesure du taux drsquooxygegravene et de la permeacuteabiliteacute de lrsquoemballage

Le transmetteur Fibox 4 trace est lrsquooutil ideacuteal pour les tests dans lrsquoindustrie du packaging et la recherche et le controcircle de qualiteacute des mateacuteriaux La manipulation aiseacutee du systegraveme et la vitesse rapide drsquoeacutetalonnage permettent drsquoeffectuer dans un deacutelai record la mesure de la peacuteneacutetration de loxygegravene et des taux de transmission dans des centaines de contenants et drsquoemballages sans les endommager Un seul scanning du code-barres est

suffisant pour deacutemarrer une mesure preacutecise de traces drsquooxygegravene En combinaison avec le deacutetecteur de type pSt9 qui permet des mesures allant jusquagrave 05 ppm doxygegravene le capteur Fibox permet de deacuteterminer des taux de concentration doxygegravene pour des applications tregraves sensibles ou par exemple pour des emballages conccedilus en mateacuteriaux tregraves denses qui constituent une barriegravere eacuteleveacutee - comme les panneaux disolation sous vide ou de modules de panneaux photovoltaiumlques

Drsquoautres domaines drsquoapplications

- packaging et recherche de mateacuteriaux - QC- Controcircle de qualiteacute en agro-alimentaire brasserie- Recherche en biologie- Recherche environmentalhellip- proceacutedeacutes de bioproduction

NeW Presens Fibox 4 amp Fibox 4 trace Capteur drsquooxygegravene agrave transmission par fibre optique non invasif et autonome

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NEW Catalogue 2014 gratuit disponible sur demande cpnanalisbe et teacuteleacutechargeable sur wwanalisbePREsENsCATALOg

Industry 97 11

o 2 - C o 2 - p H D e t e c t i o n

wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeDELSAPRotEiN

Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

Industry 9712

P a r t i c l e C h a r a c t e r i z a t i o n

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeDELSAPRotEiN

Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

Industry 97 13

P a r t i c l e C h a r a c t e r i z a t i o n

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeDELSAPRotEiN

Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

P a r t i c l e C h a r a c t e r i z a t i o n

wwwanalisbeInfo Ken De Roover I Tel 09 243 77 10 I flashanalisbe

ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

Industry 9716wwwanalisbe

Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

Industry 97 17wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

wwwanalisbeREADyPiLot

Industry 9718wwwanalisbe

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

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Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

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C a p i l l a r y E l e c t r o p h o r e s i s

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oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

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L i q u i d H a n d l i n g

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Industry 97 29

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

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P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

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P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

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Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

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wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

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usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

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Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

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v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

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v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

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F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

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150

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210

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240

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400

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500

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BINder - simulation environnementale seacutechage amp chauffage

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T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

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720

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CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

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NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

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Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

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La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

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ExplorerTM Series

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Industry 97 51

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JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

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pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

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Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

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bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

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bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

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Beckman Coulter Application NoteParticle Characterization amp Centrifugation Nanotoxicity

Abstract

Nanomedicine is a field common to materials scientists chemists biomedical engineers biologists and medical scientists The following application note will help researchers in this field gain insight into the inherent shortcomings that are present in traditional cellular toxicity assays and how these shortcomings are magnified when the toxicity assay involves nanomaterial Researchers will also benefit from understanding how Beckman Coulter centrifugation and particle characterization instruments can significantly improve the accuracy and workflow in both preparing nanoparticles and assessing the nanoparticles in vitro toxicity

Introduction

Nanotechnology biomedicine is an emerging field still in its nascent phase Nanoparticles including quantum dots (QDs) carbon nanotubes (CNTs) and graphene have many unique spectral features that have specific application for in vivo imaging and drug delivery1 QDs23 CNTs1 and graphene1 all fluoresce in the advantageous biological window allowing for deeper imaging with better sensitivity45 the strongly red-shifted Raman signal of CNTs has also been exploited for in vivo imaging6 High surface area and strong light absorption of CNTs and graphene also make them excellent drug delivery and photothermal therapy agents1 However toxicity characterization of biologically focused nanomaterial poses a challenge to researchers because of these unique properties

Figure 1 Images of SWCNT Optical images of single-walled carbon nanotube (a) without centrifugation and (b) with ultracentrifugation for two minutes at 55000 RpM (~131000 x g) Note the presence of black aggregated SWCNT in the sample that was not ultracentrifuged

The intrinsic fluorescence and absorption of each of these nanomaterials overlap with the absorption and fluorescence region used typically in vitro cell toxicity assays leading to inaccurate and misleading results7-9 Even more confounding is the fact that absorption fluorescence and toxicity1011 properties of these materials are greatly altered when they are aggregated together compared with individually solubilized thus toxicity studies comparing aggregated vs non-aggregated have systemic bias Aggregated nanoparticles are typically removed by long centrifugation processes ie CNTs typically undergo six-hour centrifugations at 22000 x g to pellet aggregates12-16 This application note highlights how the Beckman Coulter Optima MAX-Xp ultracentrifuge was used to effectively reduce the time to remove the aggregated nanotubes while the Vi-CELL XR from Beckman Coulter quantified cell toxicity in the presence of single-walled carbon nanotubes (SWCNT)

Carbon Nanotube Preparation

Single-walled carbon nanotubes (Sigma-Aldrich) were mixed with 02 1 2-Distearoyl-phospha tidylethanolamine-methyl-polyethyleneglycol(DSpE-mpEG 5 kDa molecular weight Laysan Bio) in 10 mL of water The solution was bath-sonicated for 30 minutes to create well-dispersed carbon nanotubes following previously established procedures Using a TLA-1202 rotor in an Optima MAX-Xp Ultra-centrifuge 5 mL of SWCNT solution was centrifuged in open-top polycarbonate centri-fuge tubes (Beckman Coulter pN 343778) at 22degC 55000 RpM (~131000 x g) for two minutes The top 650 μL of supernatant was collected with care to avoid disturbing the pelleted aggregates The ultracentrifuged SWCNT (referred to as UCFrsquod SWCNT) and the remaining uncentrifuged SWCNT (referred to as As-Made SWCNT) were concentrated using 10 kDa Amicon Ultra 05 mL Centri- fugal Filters (Millipore) with a Beckman Coulter Microfuge 20 microcentrifuge The concen-tration was quantified using a UV-Vis-NIR spectrophotometer (paradigm Molecular Devices) and the established mass extinction coefficient of SWCNTs at 808 nm of 465 Lgcm12 After concentration UCFrsquod SWCNTs and As-Made SWCNTs were dilu-ted using deionized water to concentrations of 06 mgmL 03 mgmL and 006 mgmL

Beckman Coulter Optima Max-Xp Ultracentrifuge

Beckman Coulter Microfuge 20 Microcentrifuge

Industry 97 7wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

C e n t r i f u g a t i o n

Toxicity Assay

MCF-7 breast cancer cells were plated at a density of 008 x 106 per well in a 24-well plate with 900 μL of RPMI10 FBS (Invitrogen) 24 hours before the addition of nanotubes Cell viability and growth were confirmed using one of the wells before the addition of nanotubes 100 μL of SWCNT samples were added to wells on the second day There were six SWCNT groups (n=2group) in total 006 mgmL UCFrsquod SWCNTs 006 mgmL As- Made SWCNTs 003 mgmL UCFrsquod SWCNTs 003 mgmL As-Made SWCNTs 0006 mgmL UCFrsquod SWCNTs and 0006 mgmL As-Made SWCNTs 100 μL of DSPE-mPEG only sample was added to wells serving as a control There were three surfactant buffer control groups (n=2group) in total 02 mgmL DSpE-mpEG 002 mgmL DSpE-mpEG and 0002 mgmL DSpE-mpEG Finally control 1 (n=1) was a complete control with cells left untouched and control 2 (n=1) had 100 μL of sterilized water added After 24 hours all the wells were washed with pBS trypsinized and mixed in 1 mL of pBS for counting in the Vi-CELL XR A new cell type was created in the Vi-CELL XR software to minimize the counting of aggregated nanotubes as cells percentages of viable cells were used to compare cell viability and difference in the two solutions

Figure 2 Cell Imaging MCF-7 cells were imaged under an optical microscope after 24 hours of incubation with SWCNT The cells incubated with either 006 mgmL As-Made SWNT (left image) or 006 mgmL ultracentrifuged SWNT (right image) have not yet reached confluence Black aggregates of SWNT can be seen in the image on the left these aggregates are difficult to wash away without washing away the cells as well The aggregates have absorption and fluorescence properties that will skew traditional toxicity assays

Figure 3 Viability Results At all concentrations ultracentrifuged SWCNT (designated by UC) had minimal toxicity 75 or more of the MCF-7 cells remained viable 24 hours after incubation Contrastingly SWCNT that were not centrifuged and contained aggregated species (designated by AG) had increasing toxicity toward MCF-7 cells that scaled with increasing concentration At a stock concentration of 06 mgmL corresponding to a concentration in solution with cells at 006 mgmL the aggregated SWCNTs had greater than 50 cell death

Figure 4 Size Distribution Data Single-walled carbon nanotubes after sonication in surfactant still have a number of aggregated species Size distribution determined by Dynamic Light Scattering on the DelsaMax pRO showed two broad species (red line) The first size range roughly 100 nm in diameter represents individually solubilized carbon nanotubes The second species containing mostly aggregated carbon nanotubes has a diameter peak closer to one micron in size After a two-minute ultracentrifugation the SWNT demonstrate only a single broad species at 100 nm indicating that virtually all aggregates have been removed This is further indicated by a 59 decrease in polydispersity Interestingly the zeta potential remains unchanged between aggregated and centrifuged carbon nanotubes This is most likely due to the fact that steric repulsion from the poly(ethylene glycol) surfactant provides most of the stability to the carbon nanotubes while electrostatic repulsion does not play a major role

Beckman Coulter Vi-CELL XR Cell Viability Analyser

Beckman Coulter DelsaMax pROparticle Characterization

Industry 978

C e n t r i f u g a t i o n

wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

Reacutefeacuterences

1 Liu Z et al Carbon materials for drug delivery amp cancer therapymdashMaterials Today 147 316-323 (2011)2 Hong G et al In Vivo Fluorescence Imaging with Ag2S Quantum Dots in the Second Near-Infrared RegionmdashAngewandte Chemie 12439 9956-9959 (2012)3 Levina L et al Efficient Infrared-Emitting PbS Quantum Dots Grown on DNA and Stable in Aqueous Solution and Blood plasmamdashAdvanced Materials 1715 1854-1857 (2005)4 Welsher K Sherlock S p and Dai H Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared windowmdashproceedings of the National Academy of Sciences 10822 8943-8948 (2011)5 Smith A M Mancini M C and Nie S Second window for in vivo imagingmdash Nature Nanotechnology 411 710 (2009)6 Zavaleta C et al Noninvasive Raman spectroscopy in living mice for evaluation of tumor targeting with carbon nanotubesmdashNano letters 89 2800-2805 (2008)7 Davoren M et al In vitro toxicity evaluation of single-walled carbon nanotubes on human A549 lung cellsmdashToxicology in vitro an international journal published in association with BIBRA 213 438 (2007)8 Woumlrle-Knirsch J M pulskamp K and Krug H F Oops they did it again Carbon nanotubes hoax scientists in viability assaysmdashNano letters 66 1261-1268 (2006)9 Ren H et al Toxicity of single-walled carbon nanotube How we were wrongmdashMaterials Today 131 6-8 (2010)

10 Wick p et al The degree and kind of agglomeration affect carbon nanotube cytotoxicitymdashToxicology letters 1682 121-131 (2007)11 Lewinski N Colvin V and Drezek R Cytotoxicity of nanoparticlesmdashSmall 41 26-49 (2008)12 Robinson J T et al High performance in vivo near-IR (gt 1 μm) imaging and photothermal cancer therapy with carbon nanotubesmdashNano Research 311 779-793 (2010)13 Donkor A D et al Carbon nanotubes inhibit the hemolytic activity of the pore-forming toxin pyolysinmdashNano Research 27 517-525 (2009)14 Kam N W S et al Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destructionmdash proceedings of the National Academy of Sciences 10233 11600-11605 (2005)15 Robinson J T et al In Vivo Fluorescence Imaging in the Second Near- Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor UptakemdashJournal of the American Chemical Society 13425 10664-10669 (2012)16 Ghafari p Impact of single-walled carbon nanotubes on ciliated protozoa amp bacteriamdashMasterrsquos Thesis University of Waterloo (2009)17 Grabinski C et al Effect of particle dimension on biocompatibility of carbon nanomaterialsmdashCarbon 4514 2828-2835 (2007)

ConclusionDiscussion

Aggregated nanoparticles pose a difficult problem in nano-biomedicine1011 In this study the toxicity of As- Made SWCNTs (which contained visible aggregates) was examined however this data is representative of most nanoparticles The SWCNTs were separated into two groupsmdashone group was As-Made without any purification step to remove aggregates while the second group underwent ultracentrifugation in the Beckman Coulter Optima MAX-Xp ultracentrifuge While centrifugation procedures have been shown to effectively remove aggregated nanoparticles and are a standard for purification of SWCNTs the long centrifugation times (six hours or more) at low speeds (5000 x g to 22000 x g)12-16 are a hindrance to research workflow Our new ultracentrifugation method demonstrates that a two-minute high-speed ultracentrifugation achieves the same biocompatibility and individual solubilized SWCNTs15 as the longer centrifugation timemdasha 180-fold time savings to researchers

Optical images and dynamic light scattering data taken using the DelsaMax pRO are evidence that all aggregated SWCNT have been removed by the rapid ultracentrifugation The toxicity data gathered in this study was possible due to the use of Vi-CELL XR the strong absorption of the aggregates would confound typical MTT and MMp toxicity assays The Vi-CELL XR was programmed to specifically look for spherical cells with defined outlines in a sharply delineated size range ensuring that counting of carbon nanotube aggregates as either viable or dead cells was minimized The Vi-CELL XR optimization is important because of the large number of aggregates present even after cell washing As a control ultracentrifuged nanotubes were run without cells in this trial the Vi-CELL XR did not count a single live or dead cell Aggregates show increased toxicity over ultracentrifuged SWCNT which can be attributed to poor surfactant coverage and larger size of aggregated SWCNT Aggregated SWCNT have more exposed surfactant-free surface this increased surface availability of SWCNT directly contributes to an increase in reactive oxygen species (ROS)17 Furthermore aggregated or As-Made SWCNTs are much larger on a whole as demonstrated by the dynamic light scattering data the increased SWCNT size can block cell-signaling pathways or disrupt cellular action inhibiting cell growth17 Thus it is impor tant that aggregated nanoparticles are removed before being used in vitro or in vivo10

Industry 97 9

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NeW Beckman Coulter Avanti J-26s La centrifugeuse Avanti J-26s permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils

La meilleure dans sa cateacutegorieLa centrifugeuse Avanti J-26S Xp permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils Un seul systegraveme traite 6 litres de produit de fermentation en moins de 10 minutes fournit une fraction subcellulaire agrave 82 000 x g centrifuge 24 plaques de microtitration en une fois et seacutepare des populations de cellules vivantes par eacutelutriation

Fonctionnement rapideGracircce agrave la technologie de switched reluctance (SR) drive agrave couple de deacutemarrage eacuteleveacute le temps de cycle est raccourci et il ne faut plus attendre lrsquoeacutechantillon en fin de run En outre le systegraveme de reacuteduction des frictions assure un fonctionnement plus rapide plus silencieux agrave tempeacuterature plus basse et avec une consommation drsquoeacutenergie plus basse que pour les centrifugeuses classiques

Fonctionnement seacutecuriseacuteplusieurs niveaux de bioseacutecuriteacute inteacutegreacutes au niveau des contenants (couvercles joints eacutevents) des rotors (canisters et couvercles seacutecuriseacutes) et de la centrifugeuse (filtres HEPA) A cette bioseacutecuriteacute srsquoajoute lrsquoidentification automatique des rotors pour offrir plus de seacutecuriteacute aux utilisateurs

Toujours precirct agrave lrsquoemploiMecircme si vous nrsquoutilisez pas immeacutediatement tout son potentiel la centrifugeuse Avanti J-26S Xp sera toujours precircte pour reacutepondre aux changements de besoins dans vos recherches Lrsquoassortiment de 26 rotors (parmi lesquels des rotors plus leacutegers des rotors pour centrifuger en continu et eacutelutrier ainsi qursquoune gamme complegravete de rotors BioSafe) fait que lrsquoAvanti J-26S Xp sera sans doute la seule centrifugeuse agrave haute performance dont votre laboratoire aura jamais besoin

Rotor JLA-81000 agrave angle fixe

Utilisation avec la centrifugeuse Avanti J-26S Xp et J-HC

Le systegraveme de rotor composite JLA-81000 avec des pochettes auto-scellan-tes Harvestline traite six litres complets en moins de 10 minutes Sa construc-tion leacutegegravere et les canisters amovibles le rendent tregraves commode pour le traite-ment de gros volumes Les bouteilles J-LITE et leurs couvercles de fermeture sont ideacuteaux pour les reacutecoltes bacteacuteriennes et de cultures cellulaires au deacutepart de fermenteurs pour lrsquoextraction de deacutebris cellulaires pour la purification de gros volumes de virus et polysaccharides dans le cadre de la production de vaccins ou pour la concentration de boues et la clarification de surnageants Ce rotor deacuteveloppe jusqursquoagrave 15 900 x g pour de hauts rendements horaires En permettant des seacuteparations les plus efficaces avec une capaciteacute totale de 6 litres le JLA-81000 peut traiter 36 litres par heure et est de ce fait le plus efficace des rotors disponibles sur le marcheacute

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Le transmetteur Fibox 4 trace est lrsquooutil ideacuteal pour les tests dans lrsquoindustrie du packaging et la recherche et le controcircle de qualiteacute des mateacuteriaux La manipulation aiseacutee du systegraveme et la vitesse rapide drsquoeacutetalonnage permettent drsquoeffectuer dans un deacutelai record la mesure de la peacuteneacutetration de loxygegravene et des taux de transmission dans des centaines de contenants et drsquoemballages sans les endommager Un seul scanning du code-barres est

suffisant pour deacutemarrer une mesure preacutecise de traces drsquooxygegravene En combinaison avec le deacutetecteur de type pSt9 qui permet des mesures allant jusquagrave 05 ppm doxygegravene le capteur Fibox permet de deacuteterminer des taux de concentration doxygegravene pour des applications tregraves sensibles ou par exemple pour des emballages conccedilus en mateacuteriaux tregraves denses qui constituent une barriegravere eacuteleveacutee - comme les panneaux disolation sous vide ou de modules de panneaux photovoltaiumlques

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Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

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Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

Industry 97 13

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wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

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Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

P a r t i c l e C h a r a c t e r i z a t i o n

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ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

Industry 9716wwwanalisbe

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M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

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NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

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bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

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Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

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Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

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TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

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LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

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P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

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iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

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Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

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v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

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Toxicity Assay

MCF-7 breast cancer cells were plated at a density of 008 x 106 per well in a 24-well plate with 900 μL of RPMI10 FBS (Invitrogen) 24 hours before the addition of nanotubes Cell viability and growth were confirmed using one of the wells before the addition of nanotubes 100 μL of SWCNT samples were added to wells on the second day There were six SWCNT groups (n=2group) in total 006 mgmL UCFrsquod SWCNTs 006 mgmL As- Made SWCNTs 003 mgmL UCFrsquod SWCNTs 003 mgmL As-Made SWCNTs 0006 mgmL UCFrsquod SWCNTs and 0006 mgmL As-Made SWCNTs 100 μL of DSPE-mPEG only sample was added to wells serving as a control There were three surfactant buffer control groups (n=2group) in total 02 mgmL DSpE-mpEG 002 mgmL DSpE-mpEG and 0002 mgmL DSpE-mpEG Finally control 1 (n=1) was a complete control with cells left untouched and control 2 (n=1) had 100 μL of sterilized water added After 24 hours all the wells were washed with pBS trypsinized and mixed in 1 mL of pBS for counting in the Vi-CELL XR A new cell type was created in the Vi-CELL XR software to minimize the counting of aggregated nanotubes as cells percentages of viable cells were used to compare cell viability and difference in the two solutions

Figure 2 Cell Imaging MCF-7 cells were imaged under an optical microscope after 24 hours of incubation with SWCNT The cells incubated with either 006 mgmL As-Made SWNT (left image) or 006 mgmL ultracentrifuged SWNT (right image) have not yet reached confluence Black aggregates of SWNT can be seen in the image on the left these aggregates are difficult to wash away without washing away the cells as well The aggregates have absorption and fluorescence properties that will skew traditional toxicity assays

Figure 3 Viability Results At all concentrations ultracentrifuged SWCNT (designated by UC) had minimal toxicity 75 or more of the MCF-7 cells remained viable 24 hours after incubation Contrastingly SWCNT that were not centrifuged and contained aggregated species (designated by AG) had increasing toxicity toward MCF-7 cells that scaled with increasing concentration At a stock concentration of 06 mgmL corresponding to a concentration in solution with cells at 006 mgmL the aggregated SWCNTs had greater than 50 cell death

Figure 4 Size Distribution Data Single-walled carbon nanotubes after sonication in surfactant still have a number of aggregated species Size distribution determined by Dynamic Light Scattering on the DelsaMax pRO showed two broad species (red line) The first size range roughly 100 nm in diameter represents individually solubilized carbon nanotubes The second species containing mostly aggregated carbon nanotubes has a diameter peak closer to one micron in size After a two-minute ultracentrifugation the SWNT demonstrate only a single broad species at 100 nm indicating that virtually all aggregates have been removed This is further indicated by a 59 decrease in polydispersity Interestingly the zeta potential remains unchanged between aggregated and centrifuged carbon nanotubes This is most likely due to the fact that steric repulsion from the poly(ethylene glycol) surfactant provides most of the stability to the carbon nanotubes while electrostatic repulsion does not play a major role

Beckman Coulter Vi-CELL XR Cell Viability Analyser

Beckman Coulter DelsaMax pROparticle Characterization

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Reacutefeacuterences

1 Liu Z et al Carbon materials for drug delivery amp cancer therapymdashMaterials Today 147 316-323 (2011)2 Hong G et al In Vivo Fluorescence Imaging with Ag2S Quantum Dots in the Second Near-Infrared RegionmdashAngewandte Chemie 12439 9956-9959 (2012)3 Levina L et al Efficient Infrared-Emitting PbS Quantum Dots Grown on DNA and Stable in Aqueous Solution and Blood plasmamdashAdvanced Materials 1715 1854-1857 (2005)4 Welsher K Sherlock S p and Dai H Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared windowmdashproceedings of the National Academy of Sciences 10822 8943-8948 (2011)5 Smith A M Mancini M C and Nie S Second window for in vivo imagingmdash Nature Nanotechnology 411 710 (2009)6 Zavaleta C et al Noninvasive Raman spectroscopy in living mice for evaluation of tumor targeting with carbon nanotubesmdashNano letters 89 2800-2805 (2008)7 Davoren M et al In vitro toxicity evaluation of single-walled carbon nanotubes on human A549 lung cellsmdashToxicology in vitro an international journal published in association with BIBRA 213 438 (2007)8 Woumlrle-Knirsch J M pulskamp K and Krug H F Oops they did it again Carbon nanotubes hoax scientists in viability assaysmdashNano letters 66 1261-1268 (2006)9 Ren H et al Toxicity of single-walled carbon nanotube How we were wrongmdashMaterials Today 131 6-8 (2010)

10 Wick p et al The degree and kind of agglomeration affect carbon nanotube cytotoxicitymdashToxicology letters 1682 121-131 (2007)11 Lewinski N Colvin V and Drezek R Cytotoxicity of nanoparticlesmdashSmall 41 26-49 (2008)12 Robinson J T et al High performance in vivo near-IR (gt 1 μm) imaging and photothermal cancer therapy with carbon nanotubesmdashNano Research 311 779-793 (2010)13 Donkor A D et al Carbon nanotubes inhibit the hemolytic activity of the pore-forming toxin pyolysinmdashNano Research 27 517-525 (2009)14 Kam N W S et al Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destructionmdash proceedings of the National Academy of Sciences 10233 11600-11605 (2005)15 Robinson J T et al In Vivo Fluorescence Imaging in the Second Near- Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor UptakemdashJournal of the American Chemical Society 13425 10664-10669 (2012)16 Ghafari p Impact of single-walled carbon nanotubes on ciliated protozoa amp bacteriamdashMasterrsquos Thesis University of Waterloo (2009)17 Grabinski C et al Effect of particle dimension on biocompatibility of carbon nanomaterialsmdashCarbon 4514 2828-2835 (2007)

ConclusionDiscussion

Aggregated nanoparticles pose a difficult problem in nano-biomedicine1011 In this study the toxicity of As- Made SWCNTs (which contained visible aggregates) was examined however this data is representative of most nanoparticles The SWCNTs were separated into two groupsmdashone group was As-Made without any purification step to remove aggregates while the second group underwent ultracentrifugation in the Beckman Coulter Optima MAX-Xp ultracentrifuge While centrifugation procedures have been shown to effectively remove aggregated nanoparticles and are a standard for purification of SWCNTs the long centrifugation times (six hours or more) at low speeds (5000 x g to 22000 x g)12-16 are a hindrance to research workflow Our new ultracentrifugation method demonstrates that a two-minute high-speed ultracentrifugation achieves the same biocompatibility and individual solubilized SWCNTs15 as the longer centrifugation timemdasha 180-fold time savings to researchers

Optical images and dynamic light scattering data taken using the DelsaMax pRO are evidence that all aggregated SWCNT have been removed by the rapid ultracentrifugation The toxicity data gathered in this study was possible due to the use of Vi-CELL XR the strong absorption of the aggregates would confound typical MTT and MMp toxicity assays The Vi-CELL XR was programmed to specifically look for spherical cells with defined outlines in a sharply delineated size range ensuring that counting of carbon nanotube aggregates as either viable or dead cells was minimized The Vi-CELL XR optimization is important because of the large number of aggregates present even after cell washing As a control ultracentrifuged nanotubes were run without cells in this trial the Vi-CELL XR did not count a single live or dead cell Aggregates show increased toxicity over ultracentrifuged SWCNT which can be attributed to poor surfactant coverage and larger size of aggregated SWCNT Aggregated SWCNT have more exposed surfactant-free surface this increased surface availability of SWCNT directly contributes to an increase in reactive oxygen species (ROS)17 Furthermore aggregated or As-Made SWCNTs are much larger on a whole as demonstrated by the dynamic light scattering data the increased SWCNT size can block cell-signaling pathways or disrupt cellular action inhibiting cell growth17 Thus it is impor tant that aggregated nanoparticles are removed before being used in vitro or in vivo10

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NeW Beckman Coulter Avanti J-26s La centrifugeuse Avanti J-26s permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils

La meilleure dans sa cateacutegorieLa centrifugeuse Avanti J-26S Xp permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils Un seul systegraveme traite 6 litres de produit de fermentation en moins de 10 minutes fournit une fraction subcellulaire agrave 82 000 x g centrifuge 24 plaques de microtitration en une fois et seacutepare des populations de cellules vivantes par eacutelutriation

Fonctionnement rapideGracircce agrave la technologie de switched reluctance (SR) drive agrave couple de deacutemarrage eacuteleveacute le temps de cycle est raccourci et il ne faut plus attendre lrsquoeacutechantillon en fin de run En outre le systegraveme de reacuteduction des frictions assure un fonctionnement plus rapide plus silencieux agrave tempeacuterature plus basse et avec une consommation drsquoeacutenergie plus basse que pour les centrifugeuses classiques

Fonctionnement seacutecuriseacuteplusieurs niveaux de bioseacutecuriteacute inteacutegreacutes au niveau des contenants (couvercles joints eacutevents) des rotors (canisters et couvercles seacutecuriseacutes) et de la centrifugeuse (filtres HEPA) A cette bioseacutecuriteacute srsquoajoute lrsquoidentification automatique des rotors pour offrir plus de seacutecuriteacute aux utilisateurs

Toujours precirct agrave lrsquoemploiMecircme si vous nrsquoutilisez pas immeacutediatement tout son potentiel la centrifugeuse Avanti J-26S Xp sera toujours precircte pour reacutepondre aux changements de besoins dans vos recherches Lrsquoassortiment de 26 rotors (parmi lesquels des rotors plus leacutegers des rotors pour centrifuger en continu et eacutelutrier ainsi qursquoune gamme complegravete de rotors BioSafe) fait que lrsquoAvanti J-26S Xp sera sans doute la seule centrifugeuse agrave haute performance dont votre laboratoire aura jamais besoin

Rotor JLA-81000 agrave angle fixe

Utilisation avec la centrifugeuse Avanti J-26S Xp et J-HC

Le systegraveme de rotor composite JLA-81000 avec des pochettes auto-scellan-tes Harvestline traite six litres complets en moins de 10 minutes Sa construc-tion leacutegegravere et les canisters amovibles le rendent tregraves commode pour le traite-ment de gros volumes Les bouteilles J-LITE et leurs couvercles de fermeture sont ideacuteaux pour les reacutecoltes bacteacuteriennes et de cultures cellulaires au deacutepart de fermenteurs pour lrsquoextraction de deacutebris cellulaires pour la purification de gros volumes de virus et polysaccharides dans le cadre de la production de vaccins ou pour la concentration de boues et la clarification de surnageants Ce rotor deacuteveloppe jusqursquoagrave 15 900 x g pour de hauts rendements horaires En permettant des seacuteparations les plus efficaces avec une capaciteacute totale de 6 litres le JLA-81000 peut traiter 36 litres par heure et est de ce fait le plus efficace des rotors disponibles sur le marcheacute

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Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

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Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

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Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

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ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

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single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

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Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

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M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

wwwanalisbeREADyPiLot

Industry 9718wwwanalisbe

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

wwwanalisbePA800

Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

entiegraverementautoclavables(121degC100kPa20min)etpeuventecirctresteacuteriliseacutees

sansrisqueauxUV

Industry 97 27

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wwwanalisbeInfo Maria Fraile I Teacutel 09 243 77 12 I mfeanalisbe

ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

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P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

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Reacutefeacuterences

1 Liu Z et al Carbon materials for drug delivery amp cancer therapymdashMaterials Today 147 316-323 (2011)2 Hong G et al In Vivo Fluorescence Imaging with Ag2S Quantum Dots in the Second Near-Infrared RegionmdashAngewandte Chemie 12439 9956-9959 (2012)3 Levina L et al Efficient Infrared-Emitting PbS Quantum Dots Grown on DNA and Stable in Aqueous Solution and Blood plasmamdashAdvanced Materials 1715 1854-1857 (2005)4 Welsher K Sherlock S p and Dai H Deep-tissue anatomical imaging of mice using carbon nanotube fluorophores in the second near-infrared windowmdashproceedings of the National Academy of Sciences 10822 8943-8948 (2011)5 Smith A M Mancini M C and Nie S Second window for in vivo imagingmdash Nature Nanotechnology 411 710 (2009)6 Zavaleta C et al Noninvasive Raman spectroscopy in living mice for evaluation of tumor targeting with carbon nanotubesmdashNano letters 89 2800-2805 (2008)7 Davoren M et al In vitro toxicity evaluation of single-walled carbon nanotubes on human A549 lung cellsmdashToxicology in vitro an international journal published in association with BIBRA 213 438 (2007)8 Woumlrle-Knirsch J M pulskamp K and Krug H F Oops they did it again Carbon nanotubes hoax scientists in viability assaysmdashNano letters 66 1261-1268 (2006)9 Ren H et al Toxicity of single-walled carbon nanotube How we were wrongmdashMaterials Today 131 6-8 (2010)

10 Wick p et al The degree and kind of agglomeration affect carbon nanotube cytotoxicitymdashToxicology letters 1682 121-131 (2007)11 Lewinski N Colvin V and Drezek R Cytotoxicity of nanoparticlesmdashSmall 41 26-49 (2008)12 Robinson J T et al High performance in vivo near-IR (gt 1 μm) imaging and photothermal cancer therapy with carbon nanotubesmdashNano Research 311 779-793 (2010)13 Donkor A D et al Carbon nanotubes inhibit the hemolytic activity of the pore-forming toxin pyolysinmdashNano Research 27 517-525 (2009)14 Kam N W S et al Carbon nanotubes as multifunctional biological transporters and near-infrared agents for selective cancer cell destructionmdash proceedings of the National Academy of Sciences 10233 11600-11605 (2005)15 Robinson J T et al In Vivo Fluorescence Imaging in the Second Near- Infrared Window with Long Circulating Carbon Nanotubes Capable of Ultrahigh Tumor UptakemdashJournal of the American Chemical Society 13425 10664-10669 (2012)16 Ghafari p Impact of single-walled carbon nanotubes on ciliated protozoa amp bacteriamdashMasterrsquos Thesis University of Waterloo (2009)17 Grabinski C et al Effect of particle dimension on biocompatibility of carbon nanomaterialsmdashCarbon 4514 2828-2835 (2007)

ConclusionDiscussion

Aggregated nanoparticles pose a difficult problem in nano-biomedicine1011 In this study the toxicity of As- Made SWCNTs (which contained visible aggregates) was examined however this data is representative of most nanoparticles The SWCNTs were separated into two groupsmdashone group was As-Made without any purification step to remove aggregates while the second group underwent ultracentrifugation in the Beckman Coulter Optima MAX-Xp ultracentrifuge While centrifugation procedures have been shown to effectively remove aggregated nanoparticles and are a standard for purification of SWCNTs the long centrifugation times (six hours or more) at low speeds (5000 x g to 22000 x g)12-16 are a hindrance to research workflow Our new ultracentrifugation method demonstrates that a two-minute high-speed ultracentrifugation achieves the same biocompatibility and individual solubilized SWCNTs15 as the longer centrifugation timemdasha 180-fold time savings to researchers

Optical images and dynamic light scattering data taken using the DelsaMax pRO are evidence that all aggregated SWCNT have been removed by the rapid ultracentrifugation The toxicity data gathered in this study was possible due to the use of Vi-CELL XR the strong absorption of the aggregates would confound typical MTT and MMp toxicity assays The Vi-CELL XR was programmed to specifically look for spherical cells with defined outlines in a sharply delineated size range ensuring that counting of carbon nanotube aggregates as either viable or dead cells was minimized The Vi-CELL XR optimization is important because of the large number of aggregates present even after cell washing As a control ultracentrifuged nanotubes were run without cells in this trial the Vi-CELL XR did not count a single live or dead cell Aggregates show increased toxicity over ultracentrifuged SWCNT which can be attributed to poor surfactant coverage and larger size of aggregated SWCNT Aggregated SWCNT have more exposed surfactant-free surface this increased surface availability of SWCNT directly contributes to an increase in reactive oxygen species (ROS)17 Furthermore aggregated or As-Made SWCNTs are much larger on a whole as demonstrated by the dynamic light scattering data the increased SWCNT size can block cell-signaling pathways or disrupt cellular action inhibiting cell growth17 Thus it is impor tant that aggregated nanoparticles are removed before being used in vitro or in vivo10

Industry 97 9

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La meilleure dans sa cateacutegorieLa centrifugeuse Avanti J-26S Xp permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils Un seul systegraveme traite 6 litres de produit de fermentation en moins de 10 minutes fournit une fraction subcellulaire agrave 82 000 x g centrifuge 24 plaques de microtitration en une fois et seacutepare des populations de cellules vivantes par eacutelutriation

Fonctionnement rapideGracircce agrave la technologie de switched reluctance (SR) drive agrave couple de deacutemarrage eacuteleveacute le temps de cycle est raccourci et il ne faut plus attendre lrsquoeacutechantillon en fin de run En outre le systegraveme de reacuteduction des frictions assure un fonctionnement plus rapide plus silencieux agrave tempeacuterature plus basse et avec une consommation drsquoeacutenergie plus basse que pour les centrifugeuses classiques

Fonctionnement seacutecuriseacuteplusieurs niveaux de bioseacutecuriteacute inteacutegreacutes au niveau des contenants (couvercles joints eacutevents) des rotors (canisters et couvercles seacutecuriseacutes) et de la centrifugeuse (filtres HEPA) A cette bioseacutecuriteacute srsquoajoute lrsquoidentification automatique des rotors pour offrir plus de seacutecuriteacute aux utilisateurs

Toujours precirct agrave lrsquoemploiMecircme si vous nrsquoutilisez pas immeacutediatement tout son potentiel la centrifugeuse Avanti J-26S Xp sera toujours precircte pour reacutepondre aux changements de besoins dans vos recherches Lrsquoassortiment de 26 rotors (parmi lesquels des rotors plus leacutegers des rotors pour centrifuger en continu et eacutelutrier ainsi qursquoune gamme complegravete de rotors BioSafe) fait que lrsquoAvanti J-26S Xp sera sans doute la seule centrifugeuse agrave haute performance dont votre laboratoire aura jamais besoin

Rotor JLA-81000 agrave angle fixe

Utilisation avec la centrifugeuse Avanti J-26S Xp et J-HC

Le systegraveme de rotor composite JLA-81000 avec des pochettes auto-scellan-tes Harvestline traite six litres complets en moins de 10 minutes Sa construc-tion leacutegegravere et les canisters amovibles le rendent tregraves commode pour le traite-ment de gros volumes Les bouteilles J-LITE et leurs couvercles de fermeture sont ideacuteaux pour les reacutecoltes bacteacuteriennes et de cultures cellulaires au deacutepart de fermenteurs pour lrsquoextraction de deacutebris cellulaires pour la purification de gros volumes de virus et polysaccharides dans le cadre de la production de vaccins ou pour la concentration de boues et la clarification de surnageants Ce rotor deacuteveloppe jusqursquoagrave 15 900 x g pour de hauts rendements horaires En permettant des seacuteparations les plus efficaces avec une capaciteacute totale de 6 litres le JLA-81000 peut traiter 36 litres par heure et est de ce fait le plus efficace des rotors disponibles sur le marcheacute

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Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

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Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

Industry 97 13

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Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

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ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

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single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

Industry 9716wwwanalisbe

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M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

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NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

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Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

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L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

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reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

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Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

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Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

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isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

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Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

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Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

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TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

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RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

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Page 10: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

NeW Beckman Coulter Avanti J-26s La centrifugeuse Avanti J-26s permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils

La meilleure dans sa cateacutegorieLa centrifugeuse Avanti J-26S Xp permet tellement drsquoapplications qursquoelle remplace le travail de deux ou trois anciens appareils Un seul systegraveme traite 6 litres de produit de fermentation en moins de 10 minutes fournit une fraction subcellulaire agrave 82 000 x g centrifuge 24 plaques de microtitration en une fois et seacutepare des populations de cellules vivantes par eacutelutriation

Fonctionnement rapideGracircce agrave la technologie de switched reluctance (SR) drive agrave couple de deacutemarrage eacuteleveacute le temps de cycle est raccourci et il ne faut plus attendre lrsquoeacutechantillon en fin de run En outre le systegraveme de reacuteduction des frictions assure un fonctionnement plus rapide plus silencieux agrave tempeacuterature plus basse et avec une consommation drsquoeacutenergie plus basse que pour les centrifugeuses classiques

Fonctionnement seacutecuriseacuteplusieurs niveaux de bioseacutecuriteacute inteacutegreacutes au niveau des contenants (couvercles joints eacutevents) des rotors (canisters et couvercles seacutecuriseacutes) et de la centrifugeuse (filtres HEPA) A cette bioseacutecuriteacute srsquoajoute lrsquoidentification automatique des rotors pour offrir plus de seacutecuriteacute aux utilisateurs

Toujours precirct agrave lrsquoemploiMecircme si vous nrsquoutilisez pas immeacutediatement tout son potentiel la centrifugeuse Avanti J-26S Xp sera toujours precircte pour reacutepondre aux changements de besoins dans vos recherches Lrsquoassortiment de 26 rotors (parmi lesquels des rotors plus leacutegers des rotors pour centrifuger en continu et eacutelutrier ainsi qursquoune gamme complegravete de rotors BioSafe) fait que lrsquoAvanti J-26S Xp sera sans doute la seule centrifugeuse agrave haute performance dont votre laboratoire aura jamais besoin

Rotor JLA-81000 agrave angle fixe

Utilisation avec la centrifugeuse Avanti J-26S Xp et J-HC

Le systegraveme de rotor composite JLA-81000 avec des pochettes auto-scellan-tes Harvestline traite six litres complets en moins de 10 minutes Sa construc-tion leacutegegravere et les canisters amovibles le rendent tregraves commode pour le traite-ment de gros volumes Les bouteilles J-LITE et leurs couvercles de fermeture sont ideacuteaux pour les reacutecoltes bacteacuteriennes et de cultures cellulaires au deacutepart de fermenteurs pour lrsquoextraction de deacutebris cellulaires pour la purification de gros volumes de virus et polysaccharides dans le cadre de la production de vaccins ou pour la concentration de boues et la clarification de surnageants Ce rotor deacuteveloppe jusqursquoagrave 15 900 x g pour de hauts rendements horaires En permettant des seacuteparations les plus efficaces avec une capaciteacute totale de 6 litres le JLA-81000 peut traiter 36 litres par heure et est de ce fait le plus efficace des rotors disponibles sur le marcheacute

Pour plus dinformations au sujet de ces centrifugeuses et des configurations lieacutees agrave vos applications contactez nos speacutecialistes

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Industry 9710

C e n t r i f u g a t i o n

wwwanalisbeInfo Vincent Jadin I Teacutel 081 25 50 50 I flashanalisbe

Capteur drsquooxygegravene agrave transmission par fibre optique non-invasif et autonome Le nouveau systegraveme de gestion de donneacutees et le lecteur code-barres acceacutelegravere le processus de mesure- Mesure sans aucun souci gracircce au systegraveme didentification

unique des senseurs

- Mesurer de 1 ppb agrave 100 doxygegravene avec un seul appareil

- Calibrage facile par scanning du code-barres

- Compensation de la tempeacuterature de la pression et la saliniteacute

- Tregraves bonne gestion de leacutenergie pour la mesure agrave long terme

- Le logiciel de gestion de la base de donneacutees et reacutesultats (en option) offre un controcircle simultaneacute de plusieurs appareils

Exemple drsquoapplication (drsquoautres applications sur notre site internet wwwanalisbeFIBOX)

Mesure du taux drsquooxygegravene et de la permeacuteabiliteacute de lrsquoemballage

Le transmetteur Fibox 4 trace est lrsquooutil ideacuteal pour les tests dans lrsquoindustrie du packaging et la recherche et le controcircle de qualiteacute des mateacuteriaux La manipulation aiseacutee du systegraveme et la vitesse rapide drsquoeacutetalonnage permettent drsquoeffectuer dans un deacutelai record la mesure de la peacuteneacutetration de loxygegravene et des taux de transmission dans des centaines de contenants et drsquoemballages sans les endommager Un seul scanning du code-barres est

suffisant pour deacutemarrer une mesure preacutecise de traces drsquooxygegravene En combinaison avec le deacutetecteur de type pSt9 qui permet des mesures allant jusquagrave 05 ppm doxygegravene le capteur Fibox permet de deacuteterminer des taux de concentration doxygegravene pour des applications tregraves sensibles ou par exemple pour des emballages conccedilus en mateacuteriaux tregraves denses qui constituent une barriegravere eacuteleveacutee - comme les panneaux disolation sous vide ou de modules de panneaux photovoltaiumlques

Drsquoautres domaines drsquoapplications

- packaging et recherche de mateacuteriaux - QC- Controcircle de qualiteacute en agro-alimentaire brasserie- Recherche en biologie- Recherche environmentalhellip- proceacutedeacutes de bioproduction

NeW Presens Fibox 4 amp Fibox 4 trace Capteur drsquooxygegravene agrave transmission par fibre optique non invasif et autonome

Teacuteleacutechargez notre brochure wwwanalisbeBROCHUREFIBOX

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NEW Catalogue 2014 gratuit disponible sur demande cpnanalisbe et teacuteleacutechargeable sur wwanalisbePREsENsCATALOg

Industry 97 11

o 2 - C o 2 - p H D e t e c t i o n

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Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

Industry 9712

P a r t i c l e C h a r a c t e r i z a t i o n

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

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Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

Industry 97 13

P a r t i c l e C h a r a c t e r i z a t i o n

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeDELSAPRotEiN

Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

P a r t i c l e C h a r a c t e r i z a t i o n

wwwanalisbeInfo Ken De Roover I Tel 09 243 77 10 I flashanalisbe

ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

Industry 9716wwwanalisbe

Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

Industry 97 17wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

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Industry 9718wwwanalisbe

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

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Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

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Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

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C a p i l l a r y E l e c t r o p h o r e s i s

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ACTION eacuteCHANgE

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Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

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Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

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PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

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A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

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L i q u i d H a n d l i n g

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Calibration preacutecise en 3 eacutetapes simples

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Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

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Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

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Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

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Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

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ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

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Industry 9754

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C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

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Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

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Page 11: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Capteur drsquooxygegravene agrave transmission par fibre optique non-invasif et autonome Le nouveau systegraveme de gestion de donneacutees et le lecteur code-barres acceacutelegravere le processus de mesure- Mesure sans aucun souci gracircce au systegraveme didentification

unique des senseurs

- Mesurer de 1 ppb agrave 100 doxygegravene avec un seul appareil

- Calibrage facile par scanning du code-barres

- Compensation de la tempeacuterature de la pression et la saliniteacute

- Tregraves bonne gestion de leacutenergie pour la mesure agrave long terme

- Le logiciel de gestion de la base de donneacutees et reacutesultats (en option) offre un controcircle simultaneacute de plusieurs appareils

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Mesure du taux drsquooxygegravene et de la permeacuteabiliteacute de lrsquoemballage

Le transmetteur Fibox 4 trace est lrsquooutil ideacuteal pour les tests dans lrsquoindustrie du packaging et la recherche et le controcircle de qualiteacute des mateacuteriaux La manipulation aiseacutee du systegraveme et la vitesse rapide drsquoeacutetalonnage permettent drsquoeffectuer dans un deacutelai record la mesure de la peacuteneacutetration de loxygegravene et des taux de transmission dans des centaines de contenants et drsquoemballages sans les endommager Un seul scanning du code-barres est

suffisant pour deacutemarrer une mesure preacutecise de traces drsquooxygegravene En combinaison avec le deacutetecteur de type pSt9 qui permet des mesures allant jusquagrave 05 ppm doxygegravene le capteur Fibox permet de deacuteterminer des taux de concentration doxygegravene pour des applications tregraves sensibles ou par exemple pour des emballages conccedilus en mateacuteriaux tregraves denses qui constituent une barriegravere eacuteleveacutee - comme les panneaux disolation sous vide ou de modules de panneaux photovoltaiumlques

Drsquoautres domaines drsquoapplications

- packaging et recherche de mateacuteriaux - QC- Controcircle de qualiteacute en agro-alimentaire brasserie- Recherche en biologie- Recherche environmentalhellip- proceacutedeacutes de bioproduction

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NEW Catalogue 2014 gratuit disponible sur demande cpnanalisbe et teacuteleacutechargeable sur wwanalisbePREsENsCATALOg

Industry 97 11

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Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

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Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

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Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

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ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

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single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

Industry 9716wwwanalisbe

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M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

Industry 97 17wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

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Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

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wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

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Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

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Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

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C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

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Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

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-33

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Industry 97 27

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

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L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

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Calibration preacutecise en 3 eacutetapes simples

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Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

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Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

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Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

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Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

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TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

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NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

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dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

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RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

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Industry 9730wwwanalisbe

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P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

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Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

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Industry 9732wwwanalisbe

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Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

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Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

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Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

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Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

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SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

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(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

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CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

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toP tECH F25-ME916262503

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Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

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objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

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JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

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Page 12: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

wwwanalisbeDELSAPRotEiN

Particle Characterization Nanotube Application Note

Introduction

Carbon nanotubes are an emerging material that has had in-creasing industrial application in material reinforcement and great future potential in electronics energy storage and conversion and medicine1-4 The economic impact of carbon nanotubes has been estimated at $27 billion by 20155 Discovered less than 25 years ago carbon nanotubes are cylindrical quasi- one dimen-sional structures of one (single-walled carbon nanotubes SW-CNT) or more (multi-walled carbon nanotubes MWCNT) rolled-up sheets of grapheme Carbon nanotubes have inner diameters from 07ndash2 nm and can have average lengths of several microns depending on the method of preparation Comprised solely of sp2 carbon carbon nanotubes are strongly hydrophobic They are very difficult to disperse in aqueous solutions as individual nanotubes aggregation instantly occurs without amphiphilic sur-factants present to fully coat the surface of the nanotubes

Carbon nanotubes specifically SWCNT and DWCNTs (double-walled carbon nanotubes) have several unique optical prop-erties that can only be exploited in solution including a strong Raman graphitic lsquoGrsquo band peak at 1600 cm-1 and fluorescence with excitation and emission in the Near-Infrared regionmdashboth of which have been exploited for biomedical imaging and biosens-ing46 Characterization of the SWCNTs and DWCNTs and their surfactant coating is important in solution to understand the sta-bility and aggregation of the constructs Several common char-acterization methods which are used for such measurements include dynamic light scattering zeta potential and analytical ultracentrifugation7

In this report we demonstrated the use of the DelsaMax pRO to measure the size and stability of SWCNTs DWCNTs in aqueous solutions as a function of temperature The SWCNTsDWCNTs were dispersed in 2 Sodium Dodecyl Sulfate or 2 Sodium Cholate which are common surfactants used to separate indi-vidual SWCNTs89 With the DelsaMax pRO the zeta potential and hydrodynamic radius of SWCNT and DWCNT solutions were measured reliably and repeatedly in less than 1 minute with low volumes The powerful DelsaMax analysis software allowed us to rapidly display and analyze the mobility data correlation data and regularization data in real- time so that the quality of the measurement could be quickly assessed and the measure-ment could be stopped or rerun in the case of a dust event

Experimental

Carbon nanotube preparation

In 20 mL glass scintillation vials 10 mL of water was added to 200 mg of either Sodium Dodecyl Sulfate (SDS Sigma Aldrich) or Sodium Cholate (SC Sigma Aldrich) Two mg of either SW-CNT (Sigma Aldrich) or DWCNT (Sigma Aldrich) were weighed and added to the vials as well In all four different solutions were made (SDS + SWCNT SDS + DWCNT SC + SWCNT SC + DW-CNT) Each vial was bath sonicated for 10 minutes to disperse the nanotubes

Each solution was then transferred to a 15 mL conical tube and centrifuged at 3000 rpm (1855 g average rcf) Acceleration=Max Deceleration=Max at 20deg C for 5 minutes with a JS-42 rotor in a Beckman J-6B centrifuge (equivalent to J6-MI from Beckman Coulter) Centrifugation pelleted aggregated and poorly coated nanotubes while well-coated individual nanotubes remained in the supernatant

The concentration of nanotubes in solution was 58 mgL for all measurements The mass concentration of the carbon nanotube solutions was determined by UV-Vis-NIR absorption spectro- scopy (Figure 1) using the mass extinction coefficient for SWCNT at 808 nm of 465 Lgcm11 The mass extinction coeffi-cient for DWCNT is unknown at this wavelength but it should be similar Further confirmation of the mass normalization of the nanotubes was indicated by roughly the same absorption at 400 nm This region is devoid of surfactant absorption as well as Van Hove peaks of absorption of the SWNTs12 All spectroscopy measurements were performed with a 1 cm quartz cuvette using a Beckman Coulter DU 800 Spectrophotometer

Beckman Coulter DelsaMax pROparticle Characterization

Industry 9712

P a r t i c l e C h a r a c t e r i z a t i o n

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wwwanalisbeDELSAPRotEiN

Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

Industry 97 13

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wwwanalisbeDELSAPRotEiN

Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

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ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

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single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

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M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

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M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

wwwanalisbeREADyPiLot

Industry 9718wwwanalisbe

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

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wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

wwwanalisbePA800

Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

entiegraverementautoclavables(121degC100kPa20min)etpeuventecirctresteacuteriliseacutees

sansrisqueauxUV

Industry 97 27

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wwwanalisbeInfo Maria Fraile I Teacutel 09 243 77 12 I mfeanalisbe

ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

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P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

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Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

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Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

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bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

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contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

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Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

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Industry 9754

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C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

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Captairreg Flexreg XLS 392

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Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

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Page 13: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

wwwanalisbeDELSAPRotEiN

Carbon nanotube characterization

The DelsaMax pRO was used to analyze the size and stabil-ity of the carbon nanotubes solutions using respectively Dyna-mic Light Scattering (DLS) and phase Analysis Light Scattering (PALS) Briefly after connecting the flow cell to the DelsaMax pRO and turning on the instrument (allowing 30 minutes for the laser to warm up and equilibrate) the flow cell was flushed with 3 mL of deionized 20 μm filtered water Following this ~300 μl of each nanotube solution was injected with a 1 mL syringe through the injection port After a few seconds the instrument was in equilibrium demonstrated by a smooth correlation curve on the on-instrument display

The temperature was set to 20deg C with a 1deg Cminute tempera-ture ramp rate At this time simultaneous DLS and pALS mea-surements were made - 5 acquisitions at 10 secondsacquisition were taken to determine the DLS while pALS was collected for 15 seconds at 2 V and a 10 Hz alternating electric was deter-mined by looking at the mobility curves for each of the 31 pALS detectors and the individual correlation curves for each DLS acquisition pALS data was reported as zeta potential for each solution After collecting size and zeta potential measurements at 20deg C the DelsaMax pRO was ramped up to 30deg C and the size and zeta potential were re-collected This procedure contin-ued at 40deg C and 50deg C for each nanotube-surfactant solution (Figure 2)

Figure 1 UV-Vis-NIR absorption spectrum of the nanotube solutions Nanotube solutions were scanned from 250 nm to 900 nm and normalized to the same absorption at 808 nm Note the peaks of each SWCNT dispersion are due to Van Hove singularities a property of linear crystals113 Metallic SWCNTs (13 of bulk SWCNTs) have Van Hove singularities from ~400ndash550 nm while semiconducting SWCNTs (23 of bulk SWCNTs) have Van Hove singularities from 550 nm to beyond 1 micron DWCNT lack discernable Van Hove singularities the reader is directed to the review by Shen et al for a detailed reasoning for this phenomena14

Figure 2 Dynamic Light Scattering and Zeta potential Hydrodynamic radius for SWCNT and DWCNT in (a) 2 Sodium Cholate and (b) 2 Sodium Dodecyl Sulfate were determined using the DelsaMax pRO The hydrodynamic radius is the average of 10 acquisitions while the error bar corresponds to the standard deviation of the radius for the 10 acquisitions Zeta potential of SWCNT and DWCNT in (c) 2 Sodium Cholate and (d) 2 Sodium Dodecyl Sulfate did not have any observed correlation with solution temperature at all temperatures the nanotube-surfactant constructs were stable

Industry 97 13

P a r t i c l e C h a r a c t e r i z a t i o n

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeDELSAPRotEiN

Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

P a r t i c l e C h a r a c t e r i z a t i o n

wwwanalisbeInfo Ken De Roover I Tel 09 243 77 10 I flashanalisbe

ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

Industry 9716wwwanalisbe

Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

Industry 97 17wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

wwwanalisbeREADyPiLot

Industry 9718wwwanalisbe

Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

wwwanalisbePA800

Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

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P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

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wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

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P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

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v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

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Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

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C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

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Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

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wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 14: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

wwwanalisbeDELSAPRotEiN

Reacutefeacuterences

1 Jorio A Dresselhaus M S and Dresselhaus G Carbon Nanotubes Advanced Topics in the Synthesis Structure properties and ApplicationsmdashSpringer Laboratory (2008) I 2 Avouris p and Martel R progress in Carbon Nanotube Electronics and photonicsmdashMRS Bulletin 35 306-313 (2010) I 3 Baughman R H Zakhidov A A and de Heer W A Carbon nanotubes the route toward applicationsmdashScience 297 787-792 (2002) I 4 Liu Z Robinson J T Tabakman S M yang K and Dai H Carbon materials for drug delivery amp cancer therapymdashMaterials Today 14 316 (2011) I 5 ION publishing Ltd The Global Market for Carbon Nanotubes A Realistic Market Assessmentmdash2nd Edition (2010) I 6 Liu Z Tabakman S Welsher K and Dai H Carbon nanotubes in biology and medicine In vitro and in vivo detection imaging and drug deliverymdashNano Research 2 85 (2009) I 7 Maechtle W and Borger L Analytical Ultracentrifugation of polymers and NanoparticlesmdashSpringer Laboratory (2006) I 8 Green A A and Hersam M C Nearly Single-Chirality Single-Walled Carbon Nanotubes produced via Orthogonal Iterative Density Gradient UltracentrifugationmdashAdvanced Materials 23 2185

(2011) I 9 Wenseleers W Vlasov I I Goovaerts E Obraztsova E D Lobach A S and Bouwen A Efficient Isolation and Solubilization of Pristine Single-Walled Nanotubes in Bile Salt Micellesmdash Advanced Functional Materials 14 1105 (2004) I 10 Welsher K Liu Z Sherlock S p Robinson J T Chen Z Daranciang D and Dai H A route to brightly fluorescent carbon nanotubes for near-infrared imaging in micemdashNat Nano 4 773 (2009) I 11 Robinson J T Welsher K Tabakman S M Sherlock S p Wang H L Luong R and Dai H J High performance In Vivo Near- IR (gt 1 1113140m) Imaging and Photothermal Cancer Therapy with Carbon NanotubesmdashNanoResearch 3 779-793 (2010) I 12 Diao S Hong G Robinson J T Jiao L Antaris A L Wu J Z Choi C L and Dai H Chirality Enriched (121) and (113) Single- Walled Carbon Nanotubes for Biological ImagingmdashJournal of the American Chemical Society 134 16971 (2012) I 13 Van Hove L The Occurrence of Singularities in the Elastic Frequency Distribution of a Crystalmdashphysical Review 89 1189 (1953) I 14 Shen C Brozena A H and Wang y Double-walled carbon nanotubes Challenges and opportunitiesmdashNanoscale 3 503 (2010)

Results and Discussion

At 2 wv both SDS and SC were able to make stable solutions of SWCNT and DWCNT as determined by zeta potential (Figure 2c amp 2d) A zeta potential with absolute value greater than 30 mV is generally considered to be stable at all temperatures the nanotube-surfactant solutions had zeta potentials with absolute magnitude greater than 40 mV As temperature increased the hydrodynamic radius of the nanotube-surfactant constructs in-creased (Figure 2a amp 2b) This interesting result indicates that most likely the hydrophilic portion of the surfactant coating ex-panded out from the nanotube surface into solution This con-clusion is reached because the nanotubes were not changing in size with temperature and the hydrophobic portion of the surfac-tants was bound to the surface of the nanotubes Furthermore if aggregation occurred for the nanotubes at higher temperatures it would cause both a much larger than observed increase in nanotube size through the cumulants method of analysis a cor-responding decrease in nanotube stability indicated by the zeta potential approaching 0 mV (as the aggregates would begin to dominate the mobility signal) and a rapid rise in polydispersity Finally using the regularization method of analysis larger peaks in the micron region would begin to emerge at increasingly higher intensities if aggregation occurred None of these indicators were observed for any sample In all cases DWCNT were larger in radius than SWCNT due to the larger diameter the lengths of the SWCNT and DWCNT should be similar in both SC and SDS after sonication and centrifugation while zeta potential measure-ments showed a stable solution in all cases indicating that the nanotubes were individually dispersed and not aggregating

Characterizing nanoparticles in solution is crucial if the intended application requires them to be in solution While electron mi-croscopy and atomic force microscopy can give detailed infor-mation on shape and size they require that nanoparticles be deposited on a substrate which can confound the data More importantly only a small sample size of the nanoparticles can be thoroughly analyzed which can lead to a misrepresentation of the overall sample and is a long and tedious process The DelsaMax pROmdash with dual simultaneous DLS and zeta poten-tialmdashcan accurately and quickly size a solution of nanoparticles and determine the stability By performing both measurement in one low-volume flow cell (minimum volume of 140 μl and 16 mm spacing between electrodes) the solution under examina-tion does not need to be transferred which is a source of dust that can greatly affect the precision of the measurements

Despite a researcherrsquos best efforts sometimes dust or other small contaminants are included in the sample With the DelsaMax PRO software these outlying dust events are easily identified and can be removed by deselecting individual acquisitions (for DLS) or detectors (for zeta potential) Most importantly the true benefit of the simultaneous measurement is speed by collecting both zeta potential and the size within seconds the probability of a dust event is greatly reduced compared with typical size and zeta potential measurements that take at least several minutes The speed of the instrument can directly save a researcher time by getting the data quickly indirectly the time saved by minimi-zing the issue of dust contaminants may be many times greater

Industry 9714

P a r t i c l e C h a r a c t e r i z a t i o n

wwwanalisbeInfo Ken De Roover I Tel 09 243 77 10 I flashanalisbe

ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

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M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

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M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

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L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

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Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

entiegraverementautoclavables(121degC100kPa20min)etpeuventecirctresteacuteriliseacutees

sansrisqueauxUV

Industry 97 27

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

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Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

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StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

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Page 15: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

ANALytIk JeNAshort Application Hr-Cs AAs - Graphite furnacedetermination of se sb Pb and sn in natural water and mud samples

Experimental details

Sample preparation

The water samples were measured directly the mud sample was digested in aqua regia

Determination

The measurements were performed with the contrAAreg high-resolution continuum source graphite tube AAS instrument A transversely heated graphite furnace was used as the atomizer

Calibration

Pb Method of Standard Calibration concentration of the stock solution 10 μgL Pb in 1 HNO3

20 μL sample 81216 μL standard 5 μL PdMg(NO3)2 modifier stock standards 468 μgL Pb

Calibration se sn amp sb - see complete PDF

Download the complete article on our website wwwanalisbeHR-CsAAs

contrAA_series

Results

Element Sample Concentration by means of standard addition [microgL]

RSD ()

pb A 266 16B lt01 -C lt01 -88112 3943 1213787 lt01 -Qc Bove 7641 15

Se A 1710 20B lt5 -C lt5 -88112 lt5 -13787 138 18Qc Bove lt1 -

Sn A 35 20B lt2 -C 30 1588112 51 2513787 1585 28Qc Bove lt2 -

Sb A 1228 10B 38 24C 135 2688112 58 1513787 lt05 -Qc Bove 73 24

Industry 97 15

E l e m e n t a l A n a l y s i s

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

Industry 9716wwwanalisbe

Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

Industry 97 17wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

wwwanalisbeREADyPiLot

Industry 9718wwwanalisbe

Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

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reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

wwwanalisbePA800

Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

entiegraverementautoclavables(121degC100kPa20min)etpeuventecirctresteacuteriliseacutees

sansrisqueauxUV

Industry 97 27

wwwanalisbeHtLoFFERDautres promotions sur

wwwanalisbeInfo Maria Fraile I Teacutel 09 243 77 12 I mfeanalisbe

ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

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re

Fluc

tuat

ion

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Alar

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de

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ture

Cla

sse

de

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riteacute

Vent

ilate

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ilate

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agrave vi

tess

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glab

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eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

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deacutetergents liquides avec reacuteservoir exteacuterieur

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C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

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Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

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Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

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Page 16: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

single reaction Chamber (srC)

digestion in a benchtop package

Revolutionizing Microwave Digestion

The remarkable ultraWAVE features Milestonersquos unique SRC technology in a fully automated benchtop package Through advanced design and engineering Milestone has been able to reduce the size of SRC technology resulting in a small benchtop unit that can fit in even the smallest lab UltraWAVE digests up to 15 samples ndash of any matrix ndash simultaneously No need to batch samples of the same type into separate runs Eliminates labor associated with assemblydisassembly of digestion vessels ndash simply weigh the sample into a glass vial add acid and place into the sample rack

UltraWAVE is perfect for labs that have multiple sample types to digest or for labs that simply want the convenience of SRC technology The superior digestion quality and minimal blank contribution of the SRC makes UltraWAVE an ideal complement to ICp-MS The UltraWAVE with SRC technology removes the limitations of conventional closed-vessel systems and makes microwave digestion more effective and more efficient than ever before

Key Benefits of Single Reaction Chamber Technology

use of Simple Vials and RacksWith SRC a large stainless steel pressure reactor acts as both the microwave cavity and reaction vessel This reactor contains a TFM Teflon liner in which all samples are placed and subsequently subjected to the same temperature and pressure conditions prior to the digestion run the reactor is pre-pressurized As the stainless steel reactor is in itself pressurized the need for individual pressure vessels as used in standard closed-vessel digestion are no longer needed Instead samples are simply weighed into glass (disposable) quartz (reusable) or Teflon vials and loaded into a rack ndash just like loading an autosampler In fact the sample vials after digestion could be placed directly in an instrument autosampler for analysis The photograph shows an UltraWAVE sample rack loaded with 15mL glass vials following digestion Loose fitting TFM caps prevent condensation from falling into the vials but still allow pressure equalization with the chamber Sample handling is minimized which reduces the risk of contamination ndash a key advantage for ultratrace analysis by ICp-MS The separate microwave run required to clean closed reaction vessels is eliminated which greatly increases productivity Simply throw away used glass vials or clean used quartz vials in acid

High temperature and Pressure Ensures Complete DigestionAfter the reactor is sealed and prior to the microwave run the SRC is pre-pressurized with nitrogen This immediately raises the boiling point of the digestion reagents inside eliminating the potential for sample cross contamination via boiling and spitting and also prevents the loss of volatiles The SRC operates at very high temperature and pressure conditions (up to 200 bar and 300 deg C) so complete digestion of even the most difficult samples types is assured The very high pressure conditions means that much higher sample weights can be digested compared to closed-vessel systems Since all samples are in effect inside the same vessel there is direct pressure and temperature control of every sample ndash giving the best possible control of digestion conditions There is no need to use slower and less accurate indirect temperature (IR) or indirect pressure control

Digest Completely Different Samples - SimultaneouslyWith closed-vessel digestion a sample is chosen as a reference vessel temperature and pressure in that sample is directly monitored and the program adjusts power to prevent an overpressure situation This means that only samples of the same weight and type as the reference sample can be digested at the same time With SRC there is no such limitation since all samples are in the same vessel any combination of sample types and acid chemistries can be digested in the same run ndash all under the same optimal conditions For example consumer products nutraceuticals and polymer samples can be digested simultaneously greatly increasing the productivity of labs with multiple sample types to digest Unlike closed-vessel digestion there is no need to batch samples of the same matrix together for digestion Also reference materials can be digested at the same

time as samples even though the sample matrix is different enabling for the first time in-run quality control of the digestion process

Industry 9716wwwanalisbe

Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

Industry 97 17wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

wwwanalisbeREADyPiLot

Industry 9718wwwanalisbe

Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

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bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

wwwanalisbePA800

Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

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C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

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Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

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Industry 97 27

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

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L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

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strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

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Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

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Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

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TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

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Industry 9730wwwanalisbe

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P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

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Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

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Industry 9732wwwanalisbe

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Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

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Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

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720

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s

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A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 17: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

A RevolutionarySimultaneous Automated Microwave Digestion SystemAt the heart of its technology there is a large reaction sample-containing chamber pre-pressurized with inert gas and then heated by microwaves The pressurized chamber serves simultaneously as microwave cavity and reaction vessel

Capable of operating up to 199 bar pressure and 300degC

Any combination of sample types can be

digested simultaneously

Direct temperature and pressure control of every sample

No assemblydisassembly of vessels is required

Vessel cleaning is eliminated with glass vials

High capacity rotors

shorter cool down time

A new Approach to Microwave Digestion

MileStone

UltraWAVe

wwwanalisbeuLtRAWAVE

Industry 97 17wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

M i c r o w a v e S a m p l e - P r e p

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

wwwanalisbeREADyPiLot

Industry 9718wwwanalisbe

Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

wwwanalisbePA800

Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

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sansrisqueauxUV

Industry 97 27

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

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L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

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strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

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Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

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TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

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RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

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Industry 9730wwwanalisbe

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P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

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Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

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Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

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Industry 9732wwwanalisbe

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Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

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P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

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v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 18: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

NeW rAdLeys reactor-ready Pilot in detail

It is the attention to detail and wealth of innovative features that makes reactor-ready Pilot so much better

How to order Reactor-Ready Pilothellip

1 Select the Reactor-Ready Pilot Core

2 Choose the lid you require (custom options available)

3 Choose the Vessel kits you need

4 Select the overhead stirrer you need

5 If you need a thermoregulator Julabo hoses hose adapters thermofluid or accessory glassware then select from the accessory list

6 If you need automation add the Control Software and Data Hub

Stirrer support I-beam allows the stirrer drive to be raised and lowered with ease

precision ground DN200 lid and vessel flanges combine with PTFE collar and FEp encapsulated O-ring to offer superior sealing performance

FEp encapsulated O-ring and unique self centering pTFE collar to offer a leak-tight seal between the vessel and lid

Quick release pEEK coupling allows rapid exchange of vessels without the use of tools Operates over the temperature range -60˚C to +230˚C

Insulated 20mm bore vessel-to-manifold hoses are supported by the manifold and frame minimising stress on the glass sidearms

Venting stopcock on the outlet manifold allows quick and easy draining of the thermofluid during vessel exchange

Hose Tidies allow the insulated hoses to be conveniently sealed and secured when not in use

Comes as standard with both locking wheels and feet allowing the system to be fixed or mobile

Thermofluid may be drained back to the thermoregulator or decanted via a fluid drain tap on the inlet manifold

wwwanalisbeREADyPiLot

Industry 9718wwwanalisbe

Info Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

wwwanalisbePA800

Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

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P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

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wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

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P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

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v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 19: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Built-in spirit level for preciselevelling

pTFE stirrer guide provides excellent stirring and sealing performance An extended stirrer shaft support ensures whip-free agitation

patented drop-in stirrer coupling engages easily without the needfor tools

Wide range of accessory glassware including condensers dropping funnels adapters powder addition funnels gas purge and liquid inlet adapters etc

Support system accepts all leading brands of overhead stirrer and allows easy toolfree adjustment

Robust corrosion resistant framework accepts all vessel sizes without alteration

Optional polypropylene drip trays 7 22 and 45 litre options

Zero Dead Space pTFE piston is designed to prevent leaking or weeping across the full temperature range (-60ordmC to +230ordmC) Large 25mm bore minimises sample hold-up and is ideal for slurries

Aluminium vessel support collar allows vessels to sit securely in the support plate even when not clamped Matching lsquoSteprsquo on vessel neck ensures collar sits in correct position

Accepts jacketed and vacuum jacketed vessels including 5 litre 10 litre 15 litre and 20 litre

Reaction vessels are held by a novel quick-release clamp No tools are required to exchange vessels

Reactor-Ready Pilot Core system includes

- Base frame support rods vessel clamp i-beam amp stirrer support bosses

- Vessel to manifold insulated hoses with quick-release connections

- Lower inlet manifold with drain- Upper outlet manifold with vent- pTFE Stirrer guide- Stainless steel stirrer

drive coupling- One aluminium vessel

support collar- FEP flange O-ring

and pTFE centering collar

Reaction Vessel Kits make ordering vessels simple and more cost effective

These cost effective Vessel Kits include the vessel and accessories you need to get started The pt100 probe and turbine stirrer included in each kit are matched to the vessel size Each Vessel Kit includes

- Reaction Vessel with pTFE Stopcock

- Sidearm Couplings- pt100 pTFE Temperature probe

with Lemo Connector- pTFE Temperature

probe Adapter- pTFE Turbine

Stirrer

Industry 97 19wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

L a b R e a c t o r - S y n t h e s i s

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

Remplacez votre ancien systegraveme CE et eacuteconomisez

Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

maximaliseacuteebull Facile agrave utiliser

bull Entretien minimalbull Compatible avec Windows 7bull La technologie CE la plus

reacutecente

Vous deacutesirez savoir ce que vaut votre ancien appareil Contactez notre speacutecialiste Marylegravene Focant ndash Tel 081 25 50 50 ndash flashanalisbe

Gracircce agrave notre politique de prix compeacutetitif vous pouvez remplacer votre eacutelectrophoregravese capillaire pour un tout nou-veau systegraveme pA 800 plus Cela nrsquoa aucune importance srsquoil srsquoagit drsquoun ancien systegraveme de Beckman Coulter ou drsquoun instrument drsquoun autre fabricant

Lrsquoanalyseur pharmaceutique PA800 plusestunesolutioninnovanteperformanteetfiable

pourlrsquoanalysequantitativedesproduitsbiologiques

wwwanalisbePA800

Analytical for RampD and QC

Analis a deacuteveloppeacute diffeacuterentes applications pour vous aider

Teacuteleacutechargez notre brochure wwwanalisbeCEOFIXBROCHURE

Industry 9722 Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe I wwwanalisbe

Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

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P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

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wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

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P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

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v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

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vitr

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inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

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Captairreg Flexreg XLS 392

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Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

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wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 20: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

NeW radleys Findenser amp Findenser MiniMinimises risk of flooding in the laboratory amp saves water

Do you know that a single classic laboratory condenser is consuming 150 L of water every hour This represent the volume of your morning home bath How much condenser are you using During how many hours every day and how many days a year

Findenser requires no running water to operate Water is a precious resource It makes little economic or environmental sense to waste thousands of litres just to cool a single condenser

How much is water costing your laboratory

the cost of running a water condenser

1 tap running at 25 litres per minute

4 hours 8 hours 24 hoursWater consumption per day (litres) 600 1200 3600Cost per day pound1 pound2 pound6Cost per month 20 working days pound20 pound40 pound120Cost per year pound240 pound480 pound1440

Based on a flow of 25 litres per minute at a cost of 016p per litre for supply and disposal of water price from Veolia Water 2012 commercial pricing

Finned aluminium jacket- excellent thermal conductivity- high performance air-cooling- chemical-resistant anodised

surface

ground glass socket- accepts standard

tapered cones- choice of sizes

Easy clamping- use with standard

laboratory clamps- clamping kits available

Patented design- sealed assembly- encapsulated thermofluid

for maximised thermal conductivity and heat transfer

Anti-roll design- contoured edges prevent

rolling when not in use- prevents damage to

aluminium fins

What are the benefits bull No risk of flooding from

running waterbull Eliminates water purchase

and disposal costsbull For solvent volumes from

5ml up to 1 litrebull Helps meet sustainable

water reduction targets

Choice of flasks- 10ml to 2 litre

flasks- maximum solvent

volume 1 litre

ground glass cone- its standard

tapered glass sockets

- choice of sizes

Average water consumption of common household appliances compared with a Findenser and water condenser

wwwanalisbeFiNDENSER

Industry 9720

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

Industry 97 21

L a b R e a c t o r - S y n t h e s i s

wwwanalisbeInfo Marc Baudoul I Teacutel 081 25 50 50 I flashanalisbe

Pharmaceutical Applications- AnionsCationscounterionanalysispurityprofiling- CEofixtradeMSforCEMSCoupling- CEofixtradepHMEKCfordruganalysis- CEofixtradeMEKCforedulcorants- CEofixtradeCarboforanalysisofcarbohydrates

Ion Analysis- anionsCations Analysisofanionscationsorganicacidsandaliphaticamines

Applications for Food Analysis- AnionsCationsAnalysisofanionscationsorganicacidsandaliphaticamines

- CEofixtradeMEKCforedulcorantsandconservatives- Followupofcellcultureandfermentation (bycombiningdifferentkits)

Applications for Protein Analysis- CEofixtradeNTMPforproteinanalysis- CEofixtradepHforpeptideanalysis

Forensic Applications- CEofixtradeCDTforalcoholabuse- CEofixtradeMSforCEMSCoupling- CEofixtradepHandMEKCkitforanalysisofseizeddrugs- Anionsforcounter-ionanalysisofseizeddrugs- CEofixtradeCARBOforanalysisofsugarinexplosives orindrugs

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Principaux avantages du PA 800 plus compareacute aux autres CEbull Systegraveme robuste bull performance de travail

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Analytical for RampD and QC

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Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

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sansrisqueauxUV

Industry 97 27

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wwwanalisbeInfo Maria Fraile I Teacutel 09 243 77 12 I mfeanalisbe

ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

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L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

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strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

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Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

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Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

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RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

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Industry 9730wwwanalisbe

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P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

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Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

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Industry 9732wwwanalisbe

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Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

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wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

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CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

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PROMO - 20 ECoNoMy F12-ED911661203

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toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

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objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

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JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

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Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

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Page 21: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Findenser performance testingA range of solvents in identical flasks and set-ups were tested with a Findenser water condenser and air condenser to record solvent loss by weight

Compared with an air condenserFor synthesis with lsquolowrsquo boiling point solvents Findenser showed a significant improvement in solvent retention With acetone or DCM the reaction boiled dry when using an air condenser yet Findenser retained 95 of the solvent under the same conditionsFor synthesis with lsquomediumrsquo boiling point solvents Findenser delivered improved solvent retention particularly with larger volumes and high temperatures

Compared with a water condenserUnder identical conditions Findenser retained solvent to the same level as a water condenser (with the exception of diethyl ether)

How does Findenser work

- Findenser comprises of an internal glass condenser and an external finned aluminium jacket between which a small amount of water is permanently sealed

- The glass condenser design has a greater internal surface area than traditional condensers increasing heat transfer capacity

- The finned jacket fits around the glass condenser further increasing the external surface area- The result is a lsquoSUpER air condenserrsquo

radleys Findenser - performs as well as a water condenserFindenser replaces water-cooled condensers in over 95 of common chemistry applications

500ml solvent in 1000ml flask 16 hours heating 10˚C above boiling point for each solvent

Solvent Loss Water Condenser Boiling Point Solvent Solvent Loss

Findenser09 40˚C DCM 0607 55˚C MetBuEther 0404 56˚C Acetone 0302 65˚C MeOH 0202 66˚C THF 0201 77˚C EtOAc 0101 78˚C EtOH 00501 82˚C CH3CN 01

Results based on a standard Findenser

Choice of B14 B19 B24 and B29 joint sizes

Findenser Mini cross section

Findenser

B14 B19 B24 B29

neWFindenser Mini

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Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

Industry 97 23wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

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Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

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Industry 97 27

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wwwanalisbeInfo Maria Fraile I Teacutel 09 243 77 12 I mfeanalisbe

ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

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L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

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Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

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Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

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Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

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Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

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wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

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objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

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Page 22: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

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Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

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C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

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C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

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CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

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Analysis of Casein and Whey Protein in Whole 2 and skim Milk by Capillary Gel electrophoresis

Introduction

Milk is an important and popular source of nutrition that is widely consumed around the world Milk for human consumption can be obtained from a number of domesticated animals including sheep goat buffalo and cow whose milk is by far the most consumed Fresh cow milk contains approximately 35 protein 80 casein 15 whey protein as well as vitamins and lipids all of which provide necessary ingredients for growth It is well documented the composition of milk changes between breeds feed and of course species Because they make up a significant proportion of the content milk proteins have been a focus of research in order to better understand its make-up quality and health-related properties Historically these proteins have been analyzed using techniques like total nitrogen analysis spectroscopy and ELISA strategies More recently analytical technologies such as capillary electrophoresis (CE) liquid chromatography and immunochemistry have gained in popularity as methods by which to analyze these proteins Capillary zone electrophoresis (CZE) has proven to be a very important analytical tool in the phenotyping of individual cows for characterization of proteins in raw milk as well as determining whether the milk has been adulterated1 The principle of CZE is separation based on differences in mass to charge ratios of a protein and its isoforms thus providing heterogeneity information We propose that CE-SDS can be used orthogonally as an automated analytical technique by which to obtain quantitative information about milk proteins In this bulletin analysis of casein and whey protein in bovine milk (whole 2 and 1) including protein fractionation by the Rowland Method and sample preparation for CE-SDS analysis on the pA 800 plus system is described

Experimental

Protein standards

Whey proteins α-lactalbumin β-lactoglobulin and bovine serum albumin in addition to α β and κ casein standards were purchased from Sigma Aldrich and used without further purification A stock solution of whey proteins was prepared in distilled and deionized (ddi) water at a concentration of 10 mgml The casein stock solutions were also prepared in 1 x pBS (phosphate buffered saline) at a concentration of 10 mgml These solutions were stored at 4ordmC until used

Preparation of Whey proteins

Bovine whole 2 and 1 milk was purchased from a local market Casein was prepared by precipitation using HCl (Masci et al2) A 10 ml volume of each milk sample was brought to room temperature and adjusted to pH 40 using 20 M HCl while subjected to constant shaking The resulting curdled material corresponding to the casein fraction was separated from the whey fraction by centrifugation at 1800g for 20 min at room

temperature One ml of 02 M acetate buffer pH 40 was added to 2 ml of whey and stored at room temperature for 15 min then centrifuged for 15 minutes at 1800g also at room temperature The supernatant was recovered and filtered through a 045 μm syringe filter The resulting solution was diluted by mixing an equal volume of the supernatant and ddi water and stored at 4ordmC until use

Preparation of whey protein for CE SDS analysis

The Beckman Coulter SDS MW Assay (Beckman Coulter pN 390953) was used in the preparation of samples for separation on the pA 800 plus instrument Fifty microliters of sample were thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal marker and 5 μl of beta-mercaptoethanol and then heated at 100ordmC for 3 minutes

Preparation of Caseins

The casein fraction was treated as described by Olieman et al3 The precipitate was washed 3 times with 20 ml of acetate buffer (4 acetic acid and 04 M sodium acetate pH 46) In the case of the whole milk sample an extra wash with 10 ml of dichloromethane was performed to remove lipids followed by another wash of 10 ml of acetate buffer pH 46 The resulting casein clean fraction was dried collected and frozen at -20ordmC until use

Casein Sample Buffer (300 mM of tris 20 mM of EDtA 01M urea and 10 mM Dtt)

In a 50 ml flask containing 20 ml of ddi water were dispensed 182 g of Trizma base 037 g of EDTA and 03 g of Urea followed by the addition of 05 ml of 1 M DTT solution ddi water was added to a final volume of 50 ml It may take up to 15 minutes for the complete dissolution of all solids This solution must be prepared fresh daily

Preparation of Caseins for CE SDS analysis

300 mg of casein derived from the three varieties of milk was each mixed with 700 μl of the casein sample buffer The casein fraction generally does not dissolve completely and was therefore left to incubate for 30 min at room temperature The mixture was centrifuged at 14000 g for 10 min The solid was discarded and the supernatant filtered through a 045 μm syringe filter This solution was used for CE SDS analysis

Preparation of Caseins for CE SDS analysis

50 μl of each filtered sample was thoroughly mixed with 45 μl of CE SDS sample buffer 2 μl of 10 kD internal standard and 5 μl of β-mercaptoethanol This mixture was heated at 100ordmC for 3 minutes

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C a p i l l a r y E l e c t r o p h o r e s i s

Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

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C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

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C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

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- Burst testers to determine bursting resistance of paper and corrugated board

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NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

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P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

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wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

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Workflow

The sample preparation workflow for both whey and casein species is illustrated in Chart 1

instrumentation

All centrifugation steps were carried out using a Beckman Coulter Allegra X-22 Centrifuge equipped with the SX4250 rotor with tube inserts for 50 ml or 15 ml Falconreg tubes The CE SDS experiments were carried out on a pA 800 plus pharmaceutical Analysis System equipped with a pDA detector The methods used for CE SDS separations including capillary conditioning CE SDS separations and shutdown method where performed as described elsewhere4

Results and Discussion

CE SDS of whey proteins

Figure 1 illustrates CE SDS separation for whey proteins following HCl extraction from whole 2 and 1 milk and the molecular weight size ladder used to calculate their respective MW The molecular weights for these proteins were estimated based on interpolation of the observed migration time for each of the protein peaks into a quadratic fit of molecular weight size standard peak assignments were performed based on the migration time observed for the individual whey protein standards run separately (data not shown) The molecular weight values found were 13 kD 18 kD and 59 kD for α-lactalbumin β-lactoglobulin and BSA respectively These values are in very good agreement with those reported in literature5

Using CE-SDS MW it is also possible to estimate the quantity of whey proteins in milk For this purpose a calibration curve using purified commercial protein standards at various concentrations was constructedFigure 2 illustrates the dilution curve obtained for separation of these standards

Figure 1 CE-SDS separations of milk whey proteins extracted with HCl

Figure 2 Calibration curve of milk whey protein commercial standards using CE-SDS

Industry 9724wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

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Industry 97 27

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ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

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forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

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L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

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de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

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strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

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Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

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TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

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Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

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Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

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Industry 9732wwwanalisbe

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Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

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wwwanalisbeCHAtiLLoNCS

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

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wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

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Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

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CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

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wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 25: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

By interpolating the corrected area of each milk protein from the calibration curve in fig 2 the concentrations projected are in good agreement with values found in the literature for cow milk4 Table 1 shows the estimated amount of whey proteins present in the different types of milk studied compared to the average amount of whey protein found in cow milk5-7 Since the variations in the composition of milk are dependent on factors such as breed and feed this can account for the slight difference found between average values in cow milk versus the amount found using CE-SDS The difference between whole 2 and

1 milk is related to their overall fat content and these 3 types of milk must contain no less than 825 of solid-not-fat (SNF)6 SNF consists of proteins including whey caseins and others lactose carbohydrates water soluble minerals and vitamins7 Our results indicate that both whole and 2 milk contain a very similar protein composition making up the whey fraction Alternatively whey in 1 milk was present at consistently lower concentrations for each of the major whey species identified Although it is not clear why the difference may be due to losses during milk filtration steps or possibly during sample preparation

Table 1 Amount of whey protein found in whole 1 and 2 milk using CE-SDS assay

Figure 3 CE-SDS separations of caseins derived from whole 2 and 1 milk

Whey Proteins (mgml)Milk type alpha-Lactalbumin beta-Lactoglobulin BSA1 milk 044 098 0052 milk 065 44 025whole milk 062 42 02Average protein content in cow milk (ref 4) 12 32 04

Analysis of the casein fraction extracted from whole 2 and 1 milk by CE-SDS is illustrated in Figure 3

Compared to whey proteins casein content is expected to be similar between whole 1 and 2 milk Although we have not yet performed quantitative work on casein visual analysis of this fraction separated using CE-SDS revealed the different milk types were reasonably similar to one another (Fig 3)

Conclusion

The data presented here illustrates that capillary electrophoresis can be used to obtain protein separation information for complex matrices like milk This type of analysis is important as it provides a means by which to assess protein quality and quantity in the area of food safety and quality control Although we may have experienced losses during sample preparation the highly resolving separation conditions provided using the Beckman Coulter CE SDS gel in combination with the pA 800 plus instrumentation offers a robust platform by which to analyze samples like milk

Industry 97 25wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

entiegraverementautoclavables(121degC100kPa20min)etpeuventecirctresteacuteriliseacutees

sansrisqueauxUV

Industry 97 27

wwwanalisbeHtLoFFERDautres promotions sur

wwwanalisbeInfo Maria Fraile I Teacutel 09 243 77 12 I mfeanalisbe

ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

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Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 26: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Marcia Santos Staff Applications Scientist Beckman Coulter Life Sciences Brea CA USAMark Lies Marketing Manager Beckman Coulter Life Sciences Brea CA USA

1 JML Heck_ C Olieman A Schennink HJF van Valenberg MHpW Visker RCR Meuldijk ACM van Hooijdonk Intl Dairy Journal 18(2008)5482 G Cartoni F Coccioli R Jasionowska and M Masci J Chrom A 846 (1999)1353 I Recio and COlieman Electrophoresis 17(1996)12284 S Jovanovic M Barac O Macej T Vocic C Lacnjevac Sensors 7(2007)3715 Butler LJ Agricultural and Resource Economics Update 3(2)(2000)3-66 University of Guelph Dairy Science Department Website httpwwwuoguelphcafoodsciencedairyscience- and-technologydairy-chemistry-and-

physicscomposition-and-structure-overview7 B Ribadeau-Dumas and R Grappin Review Milk protein analysis Lait 69(1989)357

Chart 1 Milk Sample Preparation Workflow

Industry 9726wwwanalisbe

Info Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

C a p i l l a r y E l e c t r o p h o r e s i s

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

entiegraverementautoclavables(121degC100kPa20min)etpeuventecirctresteacuteriliseacutees

sansrisqueauxUV

Industry 97 27

wwwanalisbeHtLoFFERDautres promotions sur

wwwanalisbeInfo Maria Fraile I Teacutel 09 243 77 12 I mfeanalisbe

ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

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wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

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Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

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720

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CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 27: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

dIsCoVery ProPipette monocanale agrave volume variableLa conception de la nouvelle pipette monocanale DISCOVERy pRO a complegravetement eacuteteacute axeacutee sur trois caracteacuteristiques essentielles

bull Une prise en main correcte gracircce agrave la poigneacutee profileacutee ce qui reacuteduit la tension et la fatigue dans le poignet

bull Un meacutecanisme de piston unique qui diminue la force neacutecessaire pour eacutejecter les cocircnes de pregraves de 30

bull Lrsquoajustement du volume est rapide et aiseacute mecircme avec des gants

Poigneacutee ergonomique Convient pour chaque type de main

Meacutecanisme drsquoajustement et de verrouillage de volume qui permet de travailler dune seule main

Double systegraveme pour lrsquoajustement du volumeRapide et facile

Compteur 4 chiffresBien visible au devant de la pipetteultraleacuteger avec un minimum de force de pipetageUn confort ineacutegaleacute

Pipette multicanale agrave volume variableNouvelle construction systegraveme reacutevolutionnaire de ressortMoins de pression sur le pouceReacuteglage et verouillage du volumeCompteur agrave 4 chiffres

Entiegraverement autoclavable121degC100kpa20min

isolation thermiquedes piegraveces inteacuterieures pour une haute preacutecision

Manifold 360degUn confort optimal

Ejecteur seacutequentiel des cocircnespermet drsquoeacutejecter facilement les cocircnes

Le meacutecanisme de fixation des cocircnesassure une eacutetancheacuteiteacute parfaite des tips

Echangez vos anciennes pipettes ou vos pipettes deacutefectueuses pour une nouvelle pipette HTL Discovery Pro (mono ou multichannel) et vous profiterez drsquoune remise speacuteciale de -33 sur le prix tarif de ces pipettes

Action valable jusquau 31052014

ACTION eacuteCHANgE -33

-33

TouteslespipettesDiscoveryProsont

entiegraverementautoclavables(121degC100kPa20min)etpeuventecirctresteacuteriliseacutees

sansrisqueauxUV

Industry 97 27

wwwanalisbeHtLoFFERDautres promotions sur

wwwanalisbeInfo Maria Fraile I Teacutel 09 243 77 12 I mfeanalisbe

ACTION eacuteCHANgE

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 28: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Depuis plus de 20 ans Analis propose un service de deacutecontamination maintenance et calibration pour les pipettes micropipettes et pipettes reacutepeacutetitives monocanal ou multicanauxUne eacutequipe de personnes qualifieacutees et expeacuterimenteacutees assure le controcircle et la calibration de vos pipettes selon une meacutethode gravimeacutetrique respectant les speacutecifications des normes ISO 8655-6La maintenance permet de prolonger la dureacutee de vie de vos pipettes et un eacutetalonnage freacutequent vous permet de garantir la fiabiliteacute et la preacutecision de votre pipette Un eacutetalonnage reacutegulier est recommandeacute par les normes BpL BpF FDA et ISO

wwwanalisbePIPETTECALIBRATION

Calibration pour toutes les marques suivant les speacutecifications de la norme ISO 8655-6

La calibration effectueacutee par notre service utilise la meacutethode gravimeacutetrique conforme aux speacutecifications de la norme ISO 8655-6 sur une balance analytique calibreacutee et certifieacutee ISOIEC 17025 Une maicirctrise de la tempeacuterature agrave lrsquoaide drsquoun thermomegravetre eacutetalonneacute et lrsquoutilisation drsquoeau bi-distilleacutee assurent une meilleure eacutevaluation des volumes Un certificat reprenant tous les reacutesultats de la calibration permet une parfaite traccedilabiliteacute et comparabiliteacute des reacutesultats anteacuterieurs et futurs

oPtioN iN out en choisissant cette option vous recevrez deux certificats avant et apregraves Un premier certificat (IN) de calibration (2x4 mesures) est eacutetabli lors de la reacuteception de la pipette avant toute manipulationEnsuite apregraves la deacutecontamination la maintenance preacuteventive et la calibration selon votre package classic vous recevrez un deuxiegraveme certificat (OUT)

Deacutecontamination

Degraves la reacuteception de votre pipette celle-ci est automatiquement soumise agrave une deacutecontamination chimique et bacteacuteriologique

Maintenance preacuteventive

- Deacutemontage de la pipette- Veacuterification de lrsquoeacutetat des piegraveces usageacutees

ou deacutefectueuses- Nettoyage des piegraveces- Lubrification des joints et pistons- Remplacement automatique des

teflon-seals et 0-rings (inclus dans le forfait)- Remontage de la pipette et test de fonctionnement et de fuite

pour les marques de pipettes GILSON et HTL - piegraveces de rechange en provenance des fabricants

Deux types de package vous sont proposeacutes

PiPEtCAL Classic - La deacutecontamination chimique et bacteacuteriologique de votre

pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de 2 volumes x 4 mesures - Etablissement drsquoun certificat (OUT) reprenant tous les

reacutesultats de calibration- Ce service Classic vous garantit un eacutetat parfait agrave un coucirct

forfaitaire tregraves compeacutetitif- oPtioN inout

PiPEtCAL High - La deacutecontamination chimique et bacteacuteriologique de

votre pipette - Maintenance preacuteventive (voir descriptif ci-dessus) - Calibration avec un eacutetalonnage de

3 volumes x 10 mesures - Etablissement (OUT) drsquoun certificat reprenant tous les

reacutesultats de calibration- Ce service High vous garantit une plus grande preacutecision

et une meilleure lineacuteariteacute des reacutesultats

A quelle freacutequence devez-vous veacuterifier la calibration de vos pipettes

Au moins une ou deux fois par anneacutee ou chaque fois que les reacutesultats des controcircles de qualiteacute entraicircnent un doute sur leurs performances

depuis plus de 20 ans Analis propose un service de calibration de pipettes

Industry 9728wwwanalisbe

Info Karine Matthys I Teacutel 081 25 50 50 I flashanalisbe

L i q u i d H a n d l i n g

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 29: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Le systegraveme de calibration de pipettes PCsreg ARTEL vous apporte la solution

Le pCS de Artel est un systegraveme universel vous permettant de calibrer les pipettes monocanal et de veacuterifier des volumes allant de 01 μl agrave 5000 μl Vous pouvez controcircler agrave tout moment de faccedilon rapide et facile la justesse et la preacutecision des volumes de vos pipettes Gracircce agrave ces calibrations simples et fiables vous maximisez la qualiteacute de vos transferts de liquide

Avec le systegraveme PCS vous beacuteneacutecifiez des avantages suivants

- Une veacuterification automatique de la preacutecision et de la reproductibiliteacute des volumes pipetteacutes et ce en 3 minutes seulement (10 points de mesure)

- Des reacutesultats tregraves preacutecis standardiseacutes et traccedilables selon les normes NIST

- Un rapport avec le calcul des reacutesultats des tests - Une meacutethode fiable et approuveacutee pour ameacuteliorer la technique

de pipettage - Un systegraveme sans effets environnementaux - Un systegraveme (multi users) que chaque collaborateur peut utiliser - Une meacutethode facile et plus fiable que toute autre meacutethode de

veacuterification de volumes de pipettes monocanal - Un systegraveme qui vous simplifie la conformiteacute aux speacutecifications

strictes Volumes compris entre 01 microl et 5 000 microl

Calibration preacutecise en 3 eacutetapes simples

1) Scanner la pipette2) Pipetter les eacutechantillons un par un dans la cuvette

Les reacutesultats apparaissent directement agrave lrsquoeacutecran pendant que vous pipettez

3) Analyse des reacutesultats Analyse et stockage immeacutediats des donneacutees Lrsquoeacutetiquette autocollante de calibration est imprimeacutee automatiquement

Artel Pipette tracker softwareComment informatiser vos calibrations de pipettes et la gestion de vos donneacutees et vos reacutesultats

LrsquoArtel Pipette trackertrade est un logiciel permettant la gestion complegravete du controcircle de qualiteacute de vos pipettes Il automatise la collecte de donneacutees pendant les calibrations eacutetablit des rapports et facilite la planification des calibrations toutes eacuteventuelles fautes de transcription sont eacutelimineacutees car les reacutesultats sont stockeacutes automatiquement et directement dans la base de donneacutees

Logiciel pour la gestion de vos pipettes agrave utiliser avec le ArteL PCsreg

- Affichage immeacutediat des reacutesultats volumeacutetriques- reacutesultat pass-fail immeacutediat baseacute sur vos soP- stockage automatique de toutes les donneacutees importantes- Planning automatique de la calibration des pipettes- Impression de rapport automatique via pratiquement

toute imprimante ou sauvegarde informatique- rapports faciles agrave standardiser- Base de donneacutees pour un large eacuteventail de pipettes et

leurs speacutecifications de calibration- Notification par e-mail de la prochaine calibration- Conforme aux normes GLP CLsI et Iso 9000- rapport de calibration deacutetailleacute par eacutechantillon

NoUVeAU- Creacuteer imprimer et scanner des eacutetiquettes agrave codes-barres pour les

pipettes- Creacuteer et imprimer des eacutetiquettes de calibration- Analyser les performances des pipettes et la technique de pipetage

Comment ameacuteliorer la qualiteacute de vos transferts de liquide Comment obtenir des reacutesultats toujours preacutecisreproductibles et traccedilables selon les normes NIst

wwwanalisbeInfo Marylegravene Focant I Teacutel 081 25 50 50 I flashanalisbe

wwwanalisbeARtEL

Industry 97 29

L i q u i d H a n d l i n g

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

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te

mpeacute

ratu

re

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de te

mpeacute

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plusmnK

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A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 30: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Quality Control

amp Packaging testing

These testing systems are used to verify package integrity and suitability for transportation storage and for safety and consumer protection They also validate packaging materials in product development and manufacturing to test finished products Applications include general purpose packaging pharmaceutical and medical device packaging food packaging cosmetic packaging plastic film paperboard induction seal caps and more

wwwanalisbePACKAGiNGtEStiNG

TECHLAB sysTEMs- Box compression testers

for compressive strength in packaging

- Burst testers to determine bursting resistance of paper and corrugated board

- Corrugated testers to determine crush puncture burst and permeability characteristics of various thicknesses of corrugated board samples

NEW A new range of instruments for Polymers amp Plastics testingKARg Industriektechnik - Melt Flow Indexer - Thermal Analysis of plastics amp

polymers HDT VICAT - Manual amp Automatic Notching Machine - Sample preparation for tensile testing - Allround cutters for soft and rubber

dumbell - Specimen punching Machine

- Allround cutters for soft and rubber dumbell - Specimen punching Machine

- CNC Cutter for Hard plastics - Specimen Milling Machine

- Film-paper Strip Cutter

RDM Test Equipment- Co-efficient of friction

testers- Lab and production

heat sealers to control the jaw temperature

PREsENsChemical Optical O2 pH and CO2 sensorspermeation measurement in pET bottle determination of O2 pH and CO2 content within packaging pharmaceutical vials hellip

LLOyD - Tensile bench For plastic film and paperboard crush bending burst strength strapping metal packaging compressive strength adhesion test amp adhesive bonds

CHATILLON - force gauge amp digital force testersBendflexural strength - bond strength - burst strength - crush strength - peel strength - puncture strength - snap on snap off forces - stiffness - tear strengthhellip

Also a range of apparatuses for bulk density tests pourability amp powderflow testing

wwwanalisbeKARG

Industry 9730wwwanalisbe

Info Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

P a c k a g i n g T e s t i n g

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

wwwanalisbeCHAtiLLoNCS

wwwanalisbeLLoyDLS1

Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

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P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

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wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

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Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

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mpeacute

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A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

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Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

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votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

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Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

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ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

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C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

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wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 31: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

As the worldrsquos most innovating hardness testing instruments manufacturerINNOVATEST provides fit to purpose and fit to budget hardness testing solutions all made in Europe

top quality instruments meeting or exceeding all applicable global standards such as AStM DiN-iSo JiS and GB

iNNoVAtESt testers are subjected to direct and indirect verification procedures as a final proof of compliance to standardsEach model can be complemented with a wide range of calibration blocks and indentors traceable to iSo or AStM standards

Following customers requirements iNNoVAtESt has developed a comprehensive range of accessories such as special fixtures and automatic measuring systems based on motorized 3 4 or 5 axis CNC work piece positioning

wwwanalisbeiNNoVAtESt

Industry 97 31wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

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wwwanalisbeCHAtiLLoNCS

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

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wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

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Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

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720

litre

s

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CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

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Industry 9750wwwanalisbe

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wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

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Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

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wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 32: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Spectrophotomegravetre pour une mesure fiable de la couleur - spectro-guide

La couleur et la brillance jouent un rocircle preacutepondeacuterant dans lrsquoapparence drsquoun objet Le spectro-guide est unique car il mesure les deux effets en mecircme temps Gracircce agrave la reacutesolution spectrale de 10 nm vous beacuteneacuteficiez drsquoune grande preacutecision pour vos mesures de colorimeacutetrie tant sur des surfaces sombres que des surfaces brillantes De par sa faciliteacute drsquoutilisation et le design ergonomique du spectro-guide la mesure de couleur nrsquoa jamais eacuteteacute aussi simple

NEW ByK-mac i

Le ByK-mac i permet drsquoeacutevaluer lrsquoimpression de couleur totale - dont le flip flop les effets de scin-tillement et de granulariteacute - mais donne eacutegalement de lrsquoinformation sur la formulation de pigments Le nouveau logiciel smart-chart a eacuteteacute conccedilu pour le controcircle de processus agrave base de speacutecifica-tions connues et offre une flexibiliteacute complegravete pour les exigences de laboratoire constamment en eacutevolution

Applicateur automatique de film ndash byko-drive

pour deacuteterminer les caracteacuteristiques optiques drsquoune peinture en eacutetendant une couche sur des cartes drsquoapplications standardiseacutees il est conseilleacute drsquoutiliser lrsquoapplicateur de film La

vitesse drsquoeacutetalement et la pression appliqueacutee sur lrsquoapplicateur auront un impact sur lrsquoeacutepaisseur du film la brillance lrsquoopaciteacute et la couleur Le byko-drive est un appareil eacuteconomique et facile drsquoutilisation qui est disponible avec un plateau sous vide ou avec une plaque de verre et une pince pour maintenir lrsquoeacutechantillon Diffeacuterents formats de carte et plusieurs positions marchearrecirct sont possibles et il y a un reacuteceptacle de reacutecupeacuteration de liquide pour un nettoyage plus rapide

Mesure drsquoeacutepaisseur sur film sec ndash byko-test

Lrsquoeacutepaisseur de couche de peinture est un paramegravetre essentiel agrave mesurer reacuteguliegraverement car il a un impact sur lrsquoopaciteacute de la peinture lrsquoapparence et les proprieacuteteacutes de protection drsquoun coating De couches trop eacutepaisses neacutecessitent une longue dureacutee de seacutechage et peuvent causer des fissures et des ondulations La mesure de lrsquoeacutepaisseur permet de suivre le processus de peinture et garde les principales caracteacuteristiques du coating La mesure sur film sec peut se faire de faccedilon non-destructive sur base drsquoinduction magneacutetique pour des supports ferreux et au moyen des courants de Foucault pour des supports non-ferreux Les appareils byko-test se caracteacuterisent par la reconnaissance automatique du substrat le transfert de donneacutees vers pC sans fil les capteurs sans fil la sonde inteacutegreacutee reacutesistante agrave lrsquousure avec embout hellip

NEW ByK byko-spectra effect cabine agrave lumiegravere pour un controcircle de couleur visuel

Les couleurs sont perccedilues diffeacuteremment selon les conditions drsquoillumination La cabine agrave lumiegravere est utiliseacutee pour simuler diffeacuterents types de luminositeacute afin drsquoobtenir une perception objective de la couleur Son utilisation ameacuteliore la communication augmente la confiance et reacuteduit les retours de produits Les cabines agrave lumiegravere ByK vous permettent de voir agrave quoi vos produits ressemblent reacuteellement indeacutependamment des influences du lieu et de lrsquoenvironnement

Byk Analyse de couleur

C o l o r M e a s u r e m e n t

wwwanalisbeCoLoR

Industry 9732wwwanalisbe

Info Ken De Roover amp Stefaan Dayer I Teacutel 09 243 77 10 I flashanalisbe

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

PLUg amp PLAyET voUS PoUvEz TESTER

Gracircce au choix illimiteacute des pinces et au logiciel convivial et performant le LS1 est immeacutediatement opeacutera-tionnel dans la production et pour le controcircle de qualiteacute et srsquoutilise aussi agrave des fins eacuteducatives et de RampD Les applications sont multiples des composants meacutedicaux et eacutelectroniques plastiques emballages textiles caoutchouc aliments hellip pour nrsquoen citer que quelques unes

Plusieurs essais sont possibles

Des essais de traction et de compression de flexion la deacutetermination du coefficient de friction (COF) des essais de rupture et de peeling de relaxation de tack et des tests cycliques hellip

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

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CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

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ExplorerTM Series

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Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

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bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

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recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

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deacutetergents liquides avec reacuteservoir exteacuterieur

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C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

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Action valable jusquau 15 mars 2014

Industry 9754

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C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

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de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

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Captairreg Flexreg XLS 392

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Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

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StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

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wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 33: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Chatillonreg dynamomegravetres numeacuteriques seacuterie CSLa dynamomeacutetrie nrsquoa jamais eacuteteacute aussi simple

Deacutemarrage tregraves facile

Le LS1 lsquoUniversal Testing Machinersquo est un banc de traction et de compression hautement preacutecis qui vous permet drsquoeacutevaluer les proprieacuteteacutes physiques et meacutecaniques de vos mateacuteriaux jusqursquoagrave 1 kN

Banc drsquoessais universel LS1 de Lloyd

Une SoLUTioN pour tous les BUDgETS

Un appareil CS est complegravetement opeacutera-tionnel et ne neacutecessite pas drsquoordinateur ni de logiciel

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Industry 97 33

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

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usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

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Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

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re

Fluc

tuat

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ture

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Alar

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de

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Cla

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riteacute

Vent

ilate

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ilate

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agrave vi

tess

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inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

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objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

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JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

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Page 34: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

1 Introduction

A lot of problems in industrial processes using powders are related to the poor knowledges of the dry powder behavior Indeed powder is a complex material with moving properties in time due to external factors as humidity or temperature but also due to a lot of intrinsinc powder properties such as particle size and shape porosity density roughness nature of the material Face to all these factors it is frequently difficult to establish a clear relationship between one or severals of these factors and the problems observed on the production line However a lot of these factors have significant effects on the rheological behavior of the powder So measurement of powder flowabilty and cohesion between grains (GranuDrum and GranuHeap) can give good and simple global views of a complex granular system From these information strong links can be established between simple macroscopic factors (flowability and cohesion) and behaviors observed on a process line

powders used or produced in industrial processes are often put in motion in the production line (blender air convoyering rotative furnace mixer blender dryer) powder as liquid can be characterized by different behaviors following the stress applying on it or quantity of air trapped between the particles (shear thinning or shear thickening) So it is important to determine the rheological behavior of the powder in real conditions under motion and to evaluate the evolution of the cohesion in the material under stirring Then you are able to predict and to understand the behavior of the granular material in your process For measuring the dynamic flow properties of a powder Aptis has developped the GranuDrum instrument This instrument allows to

‐ classify the samples by using simple concepts based on flowability and cohesion to choose the raw materials to optimize your process andor your material

‐ quantify the main rheological effects of the powder under motion to evaluate and predict the behavior of your material in your process line

‐ controlling the powder properties to be sure that your process is under control at every time

The cell of GranuDruM is a horizontal inox cylinder with glass side walls The capacity of the cell may vary from 10 to 100 cm3 and is half‐filled with the powder sample for analyses The cylindrical cell rotates around his axis producing the avalanches and the flow of the grains For each angular velocity several images of the GranuDruM cell containing the flowed powder are recorded as controlled time‐sampling The position of the air ndash powder interface is evolved by an edge detection The average interface position and the fluctuations around this average position are computed From the fluctuations of that interface cohesion of the

grains powder contained inside the cell and dynamic flowability can be obtained

For quality control Aptis has developped GranuHeap instrument GranuHeap is based on the formation of a heap of powder on a flat support Easy to perform and fast (60 secondes) GranuHeap allows to realize large series of measurements to control a process to analyze received raw material to select a new furnisher or even to evaluate a new composition of blends

Fig 1 shows two heaps of sugar While the granular sugar forms a classical conical heap the powdered sugar forms a strongly irregular heap Contrary to the granular sugar the powdered sugar is strongly cohesive due to the small size of the grains This example clearly shows that the heap shape strongly depends on the grain properties In particular a cohesive granular material gives a high value of the repose angle and strong deviations from the conical shape Therefore a precise measurement of the heap shape gives some useful information about interactions between the grains The angle of repose test is very sensitive to the method used to create the heap Therefore an initialization protocol has been defined in GranuHeap instrument Moreover after the heap formation the measurement of the repose angle is not obvious The shape of the heap has to be analyzed carefully

Therefore the classical method which consists in measuring the heap heigth on a circular support of diameter oslash and in calculating the angle with the relation tan(α)=2h oslash is subject to caution For our measurements an initialization tube is placed on the support After filing the tube with the sample of powder the initialization tube is removed and the powder flows from the tube to form a heap on the support and the handling of the powder by the operator is erased

The support can rotate slowly around its axis Then a camera is able to take pictures of the heap for different orientations In this way even if the heap shape is complex and asymmetric (clay lactose flour powders with high cohesion) you are able to

APtIs New and fast methods to determine powder rheology in rampd and quality control blend of sugars

Fig1 Two typical heap shapes (a) Conical heap shape obtained with a non cohesive granular sugar(b) Irregular heap of powdered sugar which is a cohesive granular material[1]

Industry 9734wwwanalisbe

Info Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

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Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

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Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

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Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

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de

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riteacute

Vent

ilate

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inteacute

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e

Con

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A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

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JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

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Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

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Industry 9754

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Page 35: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

extract all the geometrical information From each picture of the heap a dedicated algorithm finds the position of the interface powderair by image analysis The repose angle (αr) is measured by considering the pictures of the heaps of powder In addition to this parameter a cohesive index (σr) is measured Table 1 gives the empirical relation between the flow properties and the repose angle

Flow properties Repose angle (deg)Excellent 25‐30Good 31‐35Fair 36‐40passable 41‐45poor 46‐55Very poor 56‐65Very very poor gt 66

Powderedsugar (wt)

Repose angle (deg) Cohesion Height (mm) Flowproperties

0 Average 418 08 146 passableStd deviation 07 00 04

2 Average 419 08 147 passableStd deviation 06 00 05

4 Average 421 09 148 passableStd deviation 06 01 05

6 Average 458 12 165 poorStd deviation 11 02 02

8 Average 490 12 188 poorStd deviation 05 03 10

Table 2 main results obtained with GranuHeap instrument

Table 1 Empirical relation between the flow properties of a powder and repose angle[1]

[1] Measuring the flowing properties of powders and grains Powder Technology Volume 224 July 2012 Pages 19‐27

2 Applications

21 Quality control effect of small particle size on the flowability of a coarse sugar

In industrial process it is important to determine with the best accurancy the upper and the lower limits in which the process is under control to avoid production of non conform products In this exemple we consider a blend of two sugars a coarse (granular) and fine grain size (powdered) sugars We determine with GranuHeap instrument the maximum weight percent of powdered sugar (fine particles) to have in the coarse sugar to observe deleterious effects on powder flowability

Table 2 shows the main results obtained with GranuHeap instrument for different blends of sugars

As we can observe in table 2 up to 4 wt of powdered sugar the flowability of the blend keeps constant and it is not affected by the presence of the small particles (powdered sugar) in the blend Up to 4 wt of powdered sugar repose angle is about 418deg and cohesion index is about 08 Above 6 wt we can observe that repose angle and cohesion significantly increase (table 2) So powder behavior is modified by the presence of small particles in the blend Above 6 wt of fine particles we can conclude without any doubts that powder is characterized by a poor flow property which can have deleterious effect on the process (convoyering packaging sensitivity to humidity) So 4 wt of powdered sugar can be considered as the upper limit to avoid any problem on the process line GranuHeap instrument

can be used as easy and fast control quality test to ensure that process is under control by measuring flowability and cohesion of the product through repose angle

22 RampD- optimization of blend of powders (powdered milkcoffeesugar)

Blend of powders is frequenltly used in food or pharmaceutical industries In this application the blend of powders is composed of powdered milk freeze dryied coffee and sugar in ratio 206515 Two grades of sugar and coffee respectively coarse and fine have been selected to show their effects on the blend behavior

Industry 97 35wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

P o w d e r F l o w T e s t i n g

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

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Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

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mpeacute

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A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

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C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

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Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

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wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 36: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Figure 2 shows the cohesion curves obtained with GranuDrum instrument for the different blends analyzed

As we can observe cohesion for blend with coarse powders is characterized by a very robust and stable behavior (no variation) Moreover cohesion index stays very low in all the range of rotating speeds studied However replacement of coarse sugar by fine sugar (green curve) leads to an increase of the cohesion of the blend above 8 rpm However up to 8 rpm the two blends (coarse and fine sugar) are characterized by the same behavior (black and green curves) So if the stress applied to the blend is weak powder rheology of the blends can be considered as similar even if fine sugar is used Replacement of coarse sugar and coffee by fine ones leads to a very significant increase of cohesion according to the stress applied to the blend (red curve) Blend is characterized by a shear thickening behavior above 6 rpm Due to the high degree of cohesion under high stress this powder should be more difficult to convey and to pack However this powder is characterized by a low degree of segregation (fig 3) and this factor has to be kept in mind in blend achievement [2] Indeed use of fine coffee and sugar allows to obtain well dispersed coffee grains in the blend

which ensures to the customer a regular taste in its coffee cup So blend has to be optimized to meet customer wishes but also technical specifications on process line In conclusion GranuDrum instrument can be easily used to optimize and to understand rheological behavior of blend of powders under motion Moreover GranuDrum instrument can also be used to quantify the amount of additives to add in a blend of powders to improve its flowability

Fig 2 cohesion curve vs rotating speed for different blend tests

wwwanalisbePoWDERFLoW

Fig 3 respectively coarse (A) and fine (B) sugar and coffee

[2] Cascade of granular flows for characterizing segregation Powder Technology 234 (2013) 32ndash36

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe Industry 9736

P o w d e r F l o w T e s t i n g

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

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Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

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toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

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Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

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objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

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JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

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Page 37: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Using a shear cell tester to characterize

flow agents in fine powders

A small amount of flow agent can help your fine cohesive powder flow smoothly through your process without arching or ratholing But exactly how much flow agent do you need Read this article to discover how using a shear cell tester can help you quickly and accurately determine the amount of flow agent you must add to your powder to reduce or eliminate flow problems

Fine powders exhibit interparticle adhesion which often makes them cohesive This characteristic can cause several flow problems including erratic flow arching ratholing and equipment jams or blockages A common way to reduce or eliminate these flow problems is to add a small amount of a flow agent (also called a flow aid or anticaking agent) to the cohesive powder Typical flow agents include fumed silica clay talc zeolite magnesium chloride and stearic acid These materials coat the surface of the powder particles reducing or eliminating interparticle adhesion

Unfortunately in many plants a flow agent is added to a fine powder by trial and error requiring several additions of the flow agent until the powder mixture flows as desired This process is not only time-consuming and costly but subjective as well making it difficult to achieve repeatable results Adding to the challenge the acceptable amount of flow agent is typically limited to 2 percent by volume of the total powder mixture This limit based on standards set by governing bodies such as the FDA underscores the need to characterize and precisely define the amount of flow agent your cohesive powder requires

A faster less expensive and more accurate way to do this is to run a series of flow behavior tests on your powder using an automatic shear cell tester This method may also eliminate any need to test your powder mixture in a pilotscale process

Running flow function tests with a shear cell tester

By using the automatic shear cell tester to run one type of flow characterization test mdash the flow function test mdash you can quickly and accurately determine how much flow agent is needed to improve your powderrsquos flow behavior

About the shear cell tester

The automatic shear cell tester as shown in Figure 1a is a common shear cell apparatus available from various manufacturers for characterizing powder flow properties Several flow characterization tests including the flow function test are built into the testerrsquos software The software allows each test to be quickly set up and run by a lab technician without requiring extensive training Only a small powder sample is required for testing and the tester can provide comprehensive flow

characterization results in 1 to 2 hours In addition to executing the tests automatically the software does all the calculations simplifying the task of analyzing the flow data Shear cell test methods are well-defined in ASTM test methods D6128 D6682 and D67731 (1) Available from ASTM International at wwwastmorg

Flow function test method

Performing a flow function test with the shear cell tester simulates your powderrsquos flow in a bin or other vessel through an orifice The test allows you to measure how much strength (called the unconfined failure strength) the powder retains at a stress-free surface following consolidation to a given stress level (called the major principal consolidation stress) This consolidation stress level correlates with a specific fill level in a vessel Running a series of flow function tests can help you characterize the amount of flow agent that must be added to your powder to achieve reliable flow from your process equipment

Figure 1Shear cell tester

a Tester

b Trough (annular shear cell)

c Vaned lid

Industry 97 37

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

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Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

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wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

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For the initial flow function test before any flow agent is added to the powder the technician loads a powder sample into the testerrsquos trough (an annular [ring-shaped] shear cell) shown in Figure 1b After entering the samplersquos weight into the software the technician initiates the test The software automatically runs the flow function test consolidating the powder in the trough to defined strengths and shearing it horizontally with the testerrsquos vaned lid (Figure 1c) to measure the powderrsquos yield stress (that is the stress level at which it yields and flows) The results are plotted in a curve as unconfined failure strength (yield stress) versus major principal consolidation stress with one data point for each consolidation strength applied in the test

Plotting the results

The flow function test results shown in Figure 2 illustrate five regions commonly accepted by the bulk solids industry for characterizing a powder samplersquos flow behavior nonflowing very cohesive cohesive easy flowing and free flowing The red curve in this figure represents flow function test results for one powder sample with each data point representing the samplersquos flow function at that defined consolidation stress As you can see the curve shows that this powder sample is cohesive at 05 kilopascals of consolidation stress and easy flowing at higher consolidation stresses

We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

Determining the correct flow agent proportion

Once you have the initial flow function results for your powder you can run additional flow function tests on powder samples containing an increasing amount of flow agent (up to the 2 percent limit) until the powder mixture achieves the desired flow behavior By helping you accurately measure what proportion of flow agent your powder mixture requires these flow function tests provide a benchmark that future powder mixtures can be checked against

Consider this example We perform an initial flow function test with a sample of powder A a powder blend that contains no flow agent The results as illustrated by the blue curve in Figure 3 show that the material is very cohesive at various consolidation stresses This cohesiveness results in an erratic flow pattern that causes the blended powder to segregate producing an incorrect mixture We know that adding a flow agent to the powder will allow it to flow more easily without segregating but we need to determine precisely how much flow agent will produce the desired flow behavior

To do this we add a small amount of flow agent to powder A and test it again (yellow curve in Figure 3) repeating this process with more flow agent (red curve) until enough flow agent has been added to allow the powder to flow easily

To further define the correct amount of flow agent this powder requires we can use a flow index value which is a single-point measure of the powderrsquos flowability based on the flow function test results For a given flow function the flow index is a value

Figure 2 Flow function results for a powder sample

Industry 9738

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

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A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

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C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

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Captairreg Flexreg XLS 392

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Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

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MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

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wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 39: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

usually ranging from 0 to 10 that represents the inverse of the slope of a straight line running from the data point at the highest consolidation stress to the origin (where the X and y axes meet) The steeper the slope the higher the flow index and the more cohesive the powder The flow index valuersquos relationship to the five flow behavior regions is

- 0 to 010 free flowing- 010 to 025 easy flowing- 025 to 050 cohesive- 050 to 100 very cohesive- 100 and over nonflowing

Another option is to choose a flow index value at a specific consolidation strength to define the amount of flow agent we must add to the powder So for example if a powder samplersquos flow function curve shows a 019 flow index at 4 kilopascals of consolidation stress this means that the powder is easy flowing at that consolidation stress In future tests this stress value can be used to indicate that the correct amount of flow agent has been added to the powder We can also use this value to improve plant operating procedures by requiring that the vessel fill level for the powder be maintained at or above the flow index value equal to 4 kilopascals of consolidation stress

Once the flow function tests have benchmarked the correct amount of flow agent for your powder you can use the shear cell tester to check new powder mixtures against this benchmark before the mixtures go into your process

Improving your process

Beyond helping you determine how much flow agent to add to your fine cohesive powder using the shear cell tester can also help you control your process The tester can perform fast quality assurance and quality control tests to ensure that your powder is flowing as expected in your process This will catch any changes to powder flow properties caused by your process or environment before they can result in process downtime lost production or off-spec product pBE

Vinnie Hebert

Vinnie Hebert is Powder Flow Tester product manager at Brookfield Engineering Laboratories 11 Commerce Boulevard Middleboro MA 02346 800-628-8139 or 508-946-6200 ext 7171 (v_hebertbrookfield engineer ingcom wwwbrookfieldengineeringcom) He also manages product education and training at the company He holds a BS in electrical engineering from Roger Williams University in Bristol RI

For further readingFind more information on shear cell testing and flow agents in articles listed under Particle analysis and Solids flow in Powder and Bulk Engineeringrsquos article index (in the December 2012 issue and at pBErsquos website wwwpowderbulkcom) and in books available on the website at the pBE Bookstore you can also purchase copies of past pBEarticles at wwwpowderbulkcom

Figure 3 Flow function test results showing how added flow agent changes a powderrsquos flow behavior

wwwanalisbePFt

Industry 97 39

P o w d e r F l o w T e s t i n g

wwwanalisbeInfo Ken De Roover I Teacutel 09 243 77 10 I flashanalisbe

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

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Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

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A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

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Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

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StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

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Page 40: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Advancements in Instrumentation for Viscosity Measurements

The electronics revolution within the instrumentation industry has continued at an extraordinary pace as can be seen with the many recent improvements in display technology and smart operating features These advances have been especially remarkable in the latest generation of devices for measuring viscosity and yield stress User interface now includes touch-activated screens that easily access built-in libraries of reference information on test methods Accessory items for use with the instrument like temperature baths can be monitored directly Controlled user access and data security which are required to comply with standards such as 21 CFR part 11 in the US Federal Register are becoming normal features in todayrsquos viscometers and rheometers Simple and complex tests alike can execute automatically without direct operator involvement to monitor data output

So much new capability has been incorporated into todayrsquos instruments that the laboratory benchtop now has a host of time-saving and error-reduction opportunities that cannot be overlooked Why you ask What could possibly justify such an investment at this time Two answers jump right out First productivity gains are measurable and significant Second no more operator mistakes occur because the viscometer or rheometer is in control Does this mean a reduction in force for lab staff No lab techs now have time to address growing sample volume throughput and the addition of extra tests for yield stress In the case of materials like adhesives and pastry icings that need to hold their shape creep behavior can also be assessed So the bottom line is a clear return on investment that guarantees payback within the first year of operation

New rheometers like the instrument shown in Figure 1 represent the most advanced capability in the worldwide market for viscosity and yield stress measurement Ease of use is facilitated by new touchscreen interfaces which have become the standard due to the market proliferation of everyday handheld devices such as smartphones tablets etc Lab technicians can quickly access specific viscosity and yield stress test methods stored in memory in the standalone instrument Connection to a computer was necessary in the past but this is negated by the built-in computer within the instrument itself Both RampD and QC benefit because the work of RampD to create the test methods automatically transfers to QC once the methods are stored in the instrument

Viscosity test data can now appear live on the screen in graphical format with the added benefit of showing trend behavior The data on the screen as shown in Figure 2 permit visual acceptance or rejection at a glance The instrument by itself can actively monitor the measured viscosity and yield stress values and report whether the data fall between allowable QC limits established by the user These windows for data acceptability are programmed into the instrument by the user and automatically show whether the test passes or fails Interpretation of results therefore becomes automatic and provides the operator more time for other responsibilities

These improvements help to increase both the quantity and quality of data generated per test They also streamline the test in terms of the time required to execute Since the market price for these types of instruments is typically under $5000 RampD can easily afford the purchase to do the initial method development Then there is added assurance that QC can also afford the same unit due to its relatively low cost making it a double win for both departments

Figure 1 Brookfield DV3T Rheometer with touchscreen interface

Figure 2 The graphical data output on the rheometer screen characterizes material flow behavior at a glance

wwwanalisbeViSCoSity

Industry 9740wwwanalisbe

Info Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

v i s c o s i m e t r y

New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

wwwanalisbeViSCoSity

Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

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New capabilities in viscosity testing

touchscreen interface to visually observe viscosity test data

Below are some examples of the change in test methodology that becomes possible using rheometers with live screens that show real-time viscosity data in graphical format

A dairy products manufacturer realized that the live screen feature allows the operator to visually observe when the viscosity signal becomes stable and reaches a steady-state viscosity value The test can terminate at that moment instead of running for the established time interval normally on the order of several minutes The time savings over several tests every day will be significant given that there are eight instruments in the lab and 13 operators over two shifts

A minerals industry processor concluded that the torque trend indicator is a behavioral feature that would now allow viscosity testing of rapidly settling slurries (Viscometers and rheometers are fundamentally instruments used to measure torque or resistance to spindle rotation) Loss of torque which is proportional to viscosity is an indication that particles in suspension are settling out The ability to visually observe how fast this behavior occurs on the instrument screen permits improved analysis of the mixture the amount of stabilizer ingredient added to the product can be readily adjusted in order to keep particles in suspension Beneficiaries include the minerals processor and their customers alike

Built-in computer to manage viscosity data

The ability to export data from this new generation of instruments using a com port connected to a local data network or the USB port connected to a flash drive is a significant breakthrough The latter feature enables test results to be recorded in remote locations on the plant floor and transmitted via the flash drive when time permits to a central lab for recordkeeping Large plants with spray painting operations are one example in which the viscosity test results can be managed at each local spray booth but the permanent records can be captured in memory and transmitted via the flash drive at the end of each shift

Long-term viscosity tests over several hours and sometimes even days in the petroleum industry have typically involved a computer to monitor and record data This can now be done in standalone mode using todayrsquos rheometers with built-in computer Data averaging during the test is another new feature that permits quicker management of information as it is collected and processed instead of holding onto large unwieldy data files

Measurement of thixotropic index to determine flow behavior

Most QC tests for viscosity have been singlepoint measurements requiring that a spindle rotates at a defined speed for a given time interval The viscosity value is recorded and that is used to make the passfail determination for the sample Now it is just as easy to do a two-point measurement like the Thix Index test and get the extra benefit of determining the shear thinning

behavior of the sample Thix Index is the ratio of viscosity values measured at an initial speed and then at a second speed that is higher perhaps by as much as an order of magnitude For pseudoplastic materials the Thix Index will be a numerical value greater than 1 because the measured viscosity value decreases as the rotational speed increases Use of Thix Index gives a better handle on the flow behavior of the material and therefore provides added value in a QC test

integrated temperature control for QC testing

One final point worth emphasizing about viscosity measurement is the need to measure at a defined temperature RampD will always specify a test temperature when developing the test method QC needs to assure adherence to this temperature and allow sufficient time for the sample and spindle to come to temperature equilibrium The new generation of instruments can build in this temperature conditioning requirement by not allowing the test to proceed until everything has reached equilibrium In fact the rheometers and viscometers have the capability to control the apparatus which provides the temperature control (see Figure 3)

Conclusion

potential enhancements to QC test applications for viscosity measurement are many and varied It is up to the imagination of method developers in RampD and the continuous search for efficiency improvements within QC Productivity gains coupled with mistake reduction are a winndashwin proposition that will drive industrial manufacturers to upgrade to this new technology at the earliest opportunity

Robert G McGregor

Robert G McGregor MS BS is General Manager Global Marketing Brookfield Engineering Laboratories Inc 11 Commerce Blvd Middleboro MA 02346 USA tel 508-946-6200 ext 7143 e-mail r_mcgregorbrookeldengineering com wwwbrookeldengineeringcom

Figure 3 Temperature bath controlled by a Brookfield DV2T viscometer

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Industry 97 41

v i s c o s i m e t r y

wwwanalisbeInfo Erik Verheyen I Teacutel 09 243 77 10 I flashanalisbe

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

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Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

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T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 42: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Parce que

Pourquoi choisir un lyophilisateur MArtIN CHrIst

Les blocs chauffants pour toutes sortes de tubes (eppendorf falcon) maintiennent les petits eacutechantillons congeacuteleacutes pendant le tranfert du congeacutelateur vers le lyophilisateur

La chambre acrylique laisse passer la chaleur rayonnante vers lrsquoeacutechantillon ce qui augmente la vitesse de sublimation

Le capteur de vide vous tient au courant du processus dans le lyophilisateur

Le deacutegivrage actif assure un enlegravevement rapide de la glace sur le condenseur de glace

Les sondes de tempeacuterature indiquent si lrsquoeacutechantillon est congeacuteleacute etou seacutecheacute

Le controcircleur de vide et le clapet permettent drsquoacceacuteleacuterer le processus de lyophilisation et de preacutechauffer la pompe agrave vide avant que la lyophilisation deacutemarre

Opeacuteration facile via lrsquoeacutecran tactile en couleur dans sa propre langue Le panneau de controcircle signale quand il faut vidanger lrsquohuile de la pompe agrave vide

Industry 9742

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

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C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 43: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

La vanne agrave pointeau permet de reacutegler minutieusement la vitesse de ventilation pour eacuteviter qursquoun eacutechantillon sec et ultraleacuteger tourbillonne

Les plaques chauffeacutees permettent un meilleur controcircle de lrsquoapport drsquoeacutenergie vers les eacutechantillons et acceacutelegraverent ainsi la sublimation

La forme du condenseur de glace assure une surface de condensation maximale pour tenir la couche de glace aussi mince que possible Cela garantit une tempeacuterature du piegravege agrave la surface de la glace la plus basse possible pendant tout le processus

Il y a des filtres pour les reacutecipients agrave col large des valves en caoutchouc et des plateaux pour eacuteviter la contamination croiseacutee entre les eacutechantillons en cas drsquoaeacuteration du lyophilisateur

Les reacutecipients agrave col large simplifient la reacutecupeacuteration des eacutechantillons seacutecheacutes (compareacute aux ballons standard)

Un bac reacutecepteur reacutecolte lrsquoeau provenant des ballons givreacutes en fin de cycle et protegravege de cette faccedilon le lyophilisateur

wwwanalisbeMARtiNCHRiSt

Industry 97 43

F r e e z e D r y i n g

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

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C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

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wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 44: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Memmert Nouvelle geacuteneacuteration drsquoincubateurs et de fours

Sous la devise touch turn and go Memmert a introduit sur le marcheacute une nouvelle geacuteneacuteration de fours incubateurs et incubateurs refroidis par effet Peltier qui sont disponibles avec un SINGLE display et un TWIN display

La faciliteacute drsquoutilisation est la conception centrale Les deux types de controcircleurs sont drsquoun maniement absolument simple En effet une seule pression sur la touche suffit pour programmer un nouveau paramegravetre sur lrsquoeacutecran

Nouvelle uniteacute de controcircle avec TWIN-display Remarquez la nouvelle poigneacutee pratique

sINgLE DIsPLAy

La caracteacuteristique principale du sINgLE DIsPLAy est sa simpliciteacute On peut facilement lire le display agrave distance gracircce agrave la reacutesolution eacuteleveacutee et programmer les alarmes neacutecessaires (tant visuelles qursquoacoustiques) Lrsquoeacutecran est approprieacute aux applications standard et permet de suivre la vitesse du ventilateur la tempeacuterature la dureacutee et la position du volet drsquoair

TWIN DIsPLAy

Si vous deacutesirez une fonction drsquoalarmes multiple etou une programmation de vos cycles vous pouvez opter pour le TWIN DIsPLAy Vous souhaitez un e-mail vers le serveur en cas drsquoalarme Crsquoest tout agrave fait possible Efficace et tregraves sucircr De plus vous pouvez utiliser le logiciel ATMOCONTROL pour geacuterer des processus complexes plus aucun problegraveme quant agrave la traccedilabiliteacute

Memmert est une socieacuteteacute digne de confiance et efficace La nouvelle technologie vous offre plus de possibiliteacutes que jamais et gracircce au degreacute drsquoisolation plus eacuteleveacute cette nouvelle geacuteneacuteration reacutepond aux normes les plus strictes quant agrave lrsquouniformiteacute de tempeacuterature

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

T h e r m o s t a t i s a t i o n

Industry 9744

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

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C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 45: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

LaboratoryChamber amp tube Furnaces

Muffle furnaces and chamber furnaces 1100 to 1400 degC

High temperature chamber furnaces 1400 to 1800 degC

Elevator furnaces 1100 to 1800 degC

Tube furnaces (horizontalvertical) 1100 to 1800 degC

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeFuRNACE

NeW

Industry 97 45

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

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ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 46: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Materials testing at a high level

The Thermoplastic composites Research Center TpRC in the Netherlands is a research and development center that focuses on thermoplastic composites for a broad range of end use markets under the guidance of industrial companies who are members of TpRC Thermoplastic composites are important materials for many contemporary high end applications in transport industry and healthcare TpRC is primarily executing joint development projects on new thermoplastic composite technologies and applications In these joint projects TpRC takes into account materials processing and design aspects Next to the joint technology projects TpRC also executes specific developments for its members or third parties MKF 115 environmental simulation chambers and VD 115 vacuum drying ovens from BINDER are used in testing the various materials

Humidity attacks polymer composites

Most polymer composites are sensitive to moisture The mechanical properties of the polymer matrix change depending on the moisture content and the temperature

How sensitive a polymer is for moisture depends on its molecular structure and for instance the presence of polar groups Moisture leads to a decrease of stiffness and strength but may also lead to an increase of fracture toughness and ductility This is valid for thermoset as well as thermoplastic polymers Usually glass and carbon fibers become a coating to improve the bonding between the fibers and the matrix thus improving the mechanical performance These so-called sizings usually are thermoset polymers of some tenth of a micrometer thickness Next to the polymer matrix the sizing is attacked by moisture as well

We can rely on BINDER chambers 100 as any failure would mean a delay of months for us Bert Rietman Senior research associate at TpRC

Climate testing explores knockdown factors

In order to know the knock-down factors for the design process usually a number of tests are performed under different conditions This involves for instance cold temperature dry room temperature dry

Requirements

- Testing of thermoplastic composites

- Designation of humidity

- Reliable results to define knockdown factors

- Gentle circulation of air with homogeneous climate conditions

- Reliable stress tests

- Easy handling

- High quality standards

BiNDER Solutions

- Environmental simulation chamber MKF115

- precise temperature control

- Large temperature range -40 degC to 180 degC

- Humidity range 10 mdash 98 rH

- Control accessible from the front

- Vacuum drying oven VD 115

- Homogeneous climate conditions ApTlineTM

- patented expansion rack technology

- Homogeneous sample trying

- Fast condensation-free drying process

- Direct transfer of heat to the specimens due to thermal conducting plates

Research and development center TpRC

Industry 9746

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

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plusmnK

inte

rface

Alar

me

de

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e

Con

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ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 47: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Advantages

- BINDER ApTlinetrade- BINDER Cross-Flow principle- User-friendly LCD screen- Easy-to-read menu guide- Made in Germany

Areas of application

- Automotive- Electronics Semiconductor Industry- plastic Industry- Aerospace Defence Industry- Surface Technology

and hot wet testing BINDER climate chambers and vacuum ovens are employed to condition the coupons to the right moisture level In the case of wet testing conditions the coupons are fully saturated whereas in dry conditions the coupons are completely free of moisture The wide temperature range from -40 to 180 degC and humidity range from 10 mdash 98 of the MKF 115 environmental simulation chamber are of particular importance for these variable applications Just as important is use of the VD 115 vacuum drying oven from BINDER which reduces the moisture content of the materials as required We can rely on BINDER chambers 100 as any failure would mean a delay of months for us explains Bert Rietman Laboratory Manager at TpRC about their requirements These tests are a standard in aerospace industry for a long time Similar tests are used for polymers and polymer composites in the automotive industry nowadays

Ease of use and stable test conditions

The driving force for moisture uptake is diffusion Based on the moisture uptake curves which are measured using an ana-lytical balance and the time needed to obtain saturation the diffusion coefficients can be identified In the case of so-called Fickian diffusion a relatively simple mathematical expression describes the evolution of the moisture concentration Usually however this is not the case which leads to the conclusion that other mechanisms as for instance the aforementioned mois-ture transport along the interfaces play a role Together with its partners TpRC is investigating the mechanisms that play a role in moisture uptake and the influence of saturation level on

the mechanical properties of the product with the final goal of identifying reliable knock-down factors with relative ease The environmental simulation chambers and drying ovens from BINDER make it possible to precisely determine these processes ApTlinetrade in the inner chamber means even air circulation which ensures homogeneous climate conditions The research and development center particularly values how easy the chambers are to use which facilitates the daily work of the staff TpRC also values the low noise level of 62 dB Among others the company uses some of their own proprietary standards in which the ASTM and ISO standards are taken into account for testing in BINDER chambers

Designation of knockdown factors

Vacuum drying oven VD115

wwwanalisbeBiNDERMAtESt

Industry 97 47

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

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rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

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Vent

ilate

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agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

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e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

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Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

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Captairreg Flexreg XLS 392

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Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

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Page 48: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

seacuteCHAgE ET TRAITEMENT THERMIQUE

Eacutetuves de seacutechage et eacutetuves | Seacuteries ED FD et FED

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points)- Grandes reacuteserves de puissance- Temps de chauffage et de recouvrement courts- Disponible avec convection naturelle ou forceacutee

TEsT DEs MATeacuteRIAUX

Chambres drsquoessai des mateacuteriaux | Seacuteries FP et M

- Conditions de seacutechage homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Conforme agrave la norme DIN 12880 (mesure en 27 points) - Taux de renouvellement dlsquoair eacuteleveacute et grandes reacuteserves de

puissance - Temps de chauffage courts- programmation individuelle

avec controcircleur numeacuterique (uniquement seacuterie M)

Eacutetuve de seacutechage agrave convection naturelle

ED 9010-0191 9010-0079 9010-0097 - - 9010-0101 9010-0075 -

Eacutetuve de seacutechage agrave convection forceacutee

FD 9010-0194 9010-0082 9010-0102 - - 9010-0104 - -

Eacutetuve de chauffage agrave convection forceacutee

FED - 9010-0210 9010-0212 - - 9010-0214 9010-0216 -

Chambre dessai des mateacuteriaux agrave convection forceacutee

Fp - 9010-0153 9010-0255 - - 9010-0263 9010-0265 -

Chambre dessai thermique M - 9010-0201 9010-0202 - - 9010-0203 9010-0204 -

Eacutetuve de seacutechage seacutecuriseacute FDL - - 9010-0269 - - - - -

Eacutetuve de seacutechage seacutecuriseacute MDL - - 9010-0200 - - - - -

Chambres climatiques agrave conditions constantes pour les essais sous contrainte

KMF - - 9020-0187 - - 9020-0145 - -

Prod

uit

Seacuterie

23 li

tres

53 li

tres

115

litre

s

150

litre

s

210

litre

s

240

litre

s

400

litre

s

500

litre

s

BINder - simulation environnementale seacutechage amp chauffage

Industry 9748

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

700

litre

s

720

litre

s

Plag

e de

te

mpeacute

ratu

re

Fluc

tuat

ion

de te

mpeacute

ra-

ture

plusmnK

inte

rface

Alar

me

de

tem

peacutera

ture

Cla

sse

de

seacutecu

riteacute

Vent

ilate

ur

Vent

ilate

ur

agrave vi

tess

e reacute

glab

le

Porte

vitr

eacutee

inteacute

rieur

e

Con

trocircle

ur

A Acoustique V Visuel

CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 49: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

CRoiSSANCE Cultures cellulaires et tissulaires

C | CB Microbiologie BD | BF | KB | KT Cultures in vivo et in vitro

KBW | KBWF

StoCKAGE Stockage drsquoeacutechantillons UF V

SEacuteCHAGE Et CHAuFFAGE Seacutechage amp Chauffage ED | FD | FED Essai des mateacuteriaux Fp | M Seacutechage seacutecuriseacute FDL | MDL Seacutechage sous vide VD | VDL

SiMuLAtioN ENViRoNNEMENtALE Conditions climatiques constantes KBF Conditions climatiques constantes et lumiegravere

KBF p | KBF LQC Conditions constantes sous contrainte KMF

seacuteCHAgE seacuteCURIseacute

Eacutetuves de seacutechage seacutecuriseacutees | Seacuteries FDL et MDL

- Technologie de chambre de preacutechauffage avanceacutee ApTlinetrade- La quantiteacute de solvant deacutefinie respectant les normes de

seacutecuriteacute de seacutechage - Conforme EN 1539

surveillance de llsquoair frais avec arrecirct automatique - Indice de protection Ip 33 - Filtre agrave air frais facile agrave remplacer - programmation personnaliseacutee avec le controcircleur numeacuterique

(uniquement seacuterie MDL)

Chambres climatiques agrave conditions constantes pour les essais sous contrainte | Seacuterie KMF

- Conditions climatiques homogegravenes gracircce agrave la technologie de chambre de preacutechauffage avanceacutee ApTlinetrade

- Vaste plage climatique avec humiditeacute jusqulsquoagrave 90- Humidification agrave pression de vapeur avec temps

de reacuteponse rapides- Quelque soit la

qualiteacute de llsquoeau amp gestion de llsquoeacutevacuation

- Systegraveme de refroidissement puissant pour un fonctionnement sucircr jusqulsquoagrave TA 32 degC

ED - 9010-0076 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - - LEDFonction minuterieRampe

FD - - TA+ 5 degC agrave 300 degC 03 - V 2 - - LEDFonction minuterieRampe

FED - 9010-0218 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieRampe

Fp - 9010-0267 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - LEDFonction minuterieprogramme

M - 9010-0205 TA+ 5 degC agrave 300 degC 03 RS422 V 2 - programme graphique couleur

FDL - - TA+ 5 degC agrave 300 degC 03 RS422 VA 2 - - LEDFonction minuterieRampe

MDL - - TA+ 5 degC agrave 300 degC 05 RS422 VA 2 - - programme graphique couleur

KMF - 9020-0245 -10 degC agrave 100 degC lt05 Ethernet VA 31 programme graphique couleur

Seacuterie

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CONDITIONs CLIMATIQUEs CONsTANTEs sOUs CONTRAINTE

Industry 97 49

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 50: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

NEWRegloICCPompepeacuteristaltiquebidirectionelle2 3 ou 4canauxindeacutependants

une eacuteconomie de temps de coucircts amp drsquoespace Reacutevolutionnaire cettepompequitournedanslesdeuxsensenmecircmetempsvousoffreunepreacutecisionetflexibiliteacutedepompageexception-nellesaveccontrocircleindeacutependantdechaquecanal

Une seule pompe Reglo ICC remplace 4 pompes individuelles

Un deacutebit continu bidirectionnel et une distribution preacutecise des volumes

Apregraves calibration vous atteignez une preacutecision de deacutebit et une reproductibiliteacute dans la valeur-cible de 1

Une plus grande reproductibiliteacute de canal agrave canal que toute autre pompe sur le marcheacute

wwwanalisbeREGLoiCC

Industry 9750wwwanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 51: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

wwwanalisbeEXPLoRER

votre balance oHAUS PioNEERtrade (ref PA114C 80251557) 110 g 01 mg avec plateforme de 9 cm de diam et

avec calibration interne agrave 89000 euro

Action valable jusquau 30032014

+ Cotisation administrative de recyclage 010 euro (HTVA)

wwwanalisbePioNEER

Destineacutee au pesage de routine pour les diverses applications du laboratoire de lrsquoindustrie et de lrsquoenseignement la seacuterie Pioneertrade offre une parfaite combinaison entre performance et fonctionnaliteacutesModegraveles disponibles avec ou sans calibrage interne ainsi quavec lapprobation OIML

bull Cage de peseacutee avec 3 portes coulissantes sur les modegraveles 1 mg

bull Large eacuteventail doptions disponibles

bull Indicateur de niveau agrave lavant

PioneerTM SeriesAnalytical and Precision Balances

La meilleure balance pour le pesage - comptage - peseacutee en pourcentage

wwwanalisbeInfo Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe

ExplorerTM Series

NouvELLE oPTioN Portes automatiques gRATUiTESoffre speacuteciale LABoRAMAagrave voiR SUR NoTRE STAND

Industry 97 51

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 52: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

wwwanalisbeJuLABoSPECiALoFFERS

JULABo Cryostat agrave circulation pour reacutegulation de tempeacuterature en circuit interne ou externe

Ces appareils COMpACTS sont conccedilus pour des mises en tempeacuterature externes ou pour thermostater directement dans le bain Trois seacuteries dappareils couvrent toutes les gammes de puissance Lutilisation de pompes puissantes et de fortes puissances de chauffage ou de refroidissement garantissent des monteacutees ou des descentes en tempeacuterature tregraves bregraveves La grille daeacuteration amovible permet docircter deacuteventuelles poussiegraveres Selon les modegraveles ils sont eacutequipeacutes avec des poigneacutees ou des roulettes pour faciliter le transport Lorifice de vidange du bain est en faccedilade

ProMo -20

ECoNoMy - F12-ED (ref 9116612)

pour les travaux de routine et le quotidien du laboratoirebull Grand Ecran LED pour la

tempeacuterature reacuteelle et la tempeacuterature de consigne preacutecision daffichage 01degC

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film retour automatique agrave laffichage de la valeur reacuteelle

bull Reacutegulation de tempeacuterature par pID 1

bull Alarme de seacutecuriteacute de surtempeacuterature etou dassegravechement

toP tECH F12-MA (ref 9153612)

Un domaine dlsquoutilisation tregraves largebull Ecran multi-fonctions (LED) pour

tempeacuterature reacuteelle et de consigne signaux dalerte et alarmes et puissance de pompe

bull Clavier pour entrer la valeur de consigne proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de tempeacuterature par pID 2 avec compensation des perturbations

bull Calibration en 3 points ATC3bull Signal de preacute alarme pour niveau de

bain bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour communication on linebull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

toP tECH F25-ME (ref 9162625)

Avec une connexion pour sonde pt100 externe bull Affichage VFD Comfortbull Clavier pour entrer la valeur de consigne

proteacutegeacute par un film valeurs dalerte et dalarme et fonction menu

bull Reacutegulation de la tempeacuterature en cascade pID3 pour une constance de la tempeacuterature interne et externe maximale

bull Calibration en 3 points ATC3bull prise pour sonde externe pT100 pour

mesurer et pour reacutegulerbull Smart pump pompe avec reacuteglage de la

puissance par paliersbull Signal de preacute alarme pour niveau de bain

bas (DBGM 203 06 0598)bull Seacutecuriteacute de surchauffe reacuteglable agrave leacutecranbull prise RS232 pour connexion on-linebull programmateur agrave 10 pasbull Active Cooling Control utilisation de la

pleine puissance de froid sur toute la plage de tempeacuterature

PROMO - 20 ECoNoMy F12-ED911661203

toP tECH F12-MA915361203

toP tECH F25-ME916262503

Domaine de tempeacuterature (degC) -20 100 -20 200 -28 200Constance de tempeacuterature (degC) plusmn003 plusmn002 plusmn001Reacutesolution de laffichage 01 degC 001 (-9999999)01 degC 001 degCPuissance de chauffage (kW) 2 2 2

Puissance frigorifique (liquide caloporteur eacutethanol) 20degC 016 kW 016 kW 026 kW0degC 01 kW 01 kW 02 kW-20degC 002 kW 002 kW 006 kWPRiX EuRo HtVA 2 66800 3 18300 4 07300

offre valable jusqursquoau 31 mai 2014

wwwanalisbeCRyoStAtoFFER

Industry 9752

T h e r m o s t a t i s a t i o n

wwwanalisbeInfo Alain Bucovaz I Teacutel 0498 56 50 62 I flashanalisbe

+ Cotisation administrative de recyclage 010 euro (HTVA)

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 53: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

objectiveThis case study tests the heating and cooling power of the JU-LABO Presto W80 with a 10 liters glass reactor The W80 is connected to the reactor via two 10 m metal tubings The W80 is programmed to cycle between -60 degC and +100 degC

CASE STUDy

TipMake use of the option to regu-late the pump pressure You can define the desired pressure in the PRESTOreg settings

EnvironmentRoom temperature +20 degC

Humidity 45

Voltage 230 V 50 Hz

Test ConditionsJULABO unit JULABO Presto W80

Cooling power +20 degC 12 kW 0 degC 12 kW -20 degC 11 kW

Heating capacity 18 kW

Band limit No

Flow pressure 05 bar

Bath fluid JULABO Thermal HL80

Reactor 10 liters glass reactor (Normag) filled with 10 liters Thermal HL80

Control External (ICC)

Test ResultsSee chart the W80 heating process from -60 degC to +100 degC in 2 h Hitting exactly +100 degC without overshoot The cooling process from +100 degC to -60 degC in 2 h 30 min Hitting exactly -60 degC without overshoot

Nouvelle geacuteneacuteration de systegravemes de reacutegulation de tempeacuterature agrave haute dynamique JuLABo PRESToreg W91 et W92

Ces nouveaux systegravemes de mise en tempeacutera-ture agrave tregraves haute dynamique PRESTOreg W91 et W92 font partis des appareils les plus puissants du marcheacute et sont agrave la pointe des technologies de thermostatisation Ils se caracteacuterisent par leurs extrecircmes puissances de chauffage de refroidissement et de pompe Ils sont eacutequipeacutes de seacuterie de multiples options ainsi que lrsquointerface de pilotage de la nouvelle geacuteneacuteration de PRESTOreg

JULABO offre de nouvelles fonctionnaliteacutes avec le Modbus TCPiP sur la seacuterie PRESToreg avec un port Ethernet Ce qui est caracteacuteristique est le guidage interactif via lrsquoeacutecran tactile 57 cou-leur inteacutegreacute Dautres interfaces inteacutegreacutees (par exemple USB Ethernet RS232) et des inter-faces optionnelles (par exemple entreacutee standby analogique EA Profibus) offrent de nombreuses possibiliteacutes pour inteacutegrer les uniteacutes PRESTOreg dans les reacuteseaux et les systegravemes de controcircle de processus

JULABo Presto W80

WHATrsquoS NEW

CooLiNg AND HEATiNg A 10 LiTERS REACToR BETWEEN -60 degC AND +100 degC

wwwanalisbePRESTo

Ask your PREsTO amp sHILLER special Pricesabzanalisbe

Industry 97 53wwwanalisbeInfo Alain Bucovaz I Teacutel0498 56 50 62 I flashanalisbe

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 54: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Agrave lrsquoachat drsquoun nouveau systegraveme MIELE vous revevrez un chegraveque drsquoune valeur de 500 euro pour la reprise de votre vieux laveur-desinfecteur MIELE

photo non contractuelle

Laveurs-deacutesinfecteurs pour laboratoireCommande MULTiTRoNiCCommande eacutelectronique avec la posibiliteacute de programmer une deacutesinfection thermique ainsi quune deacutesinfection chimico-thermique Differents paramegravetres sont adabtables Systegraveme agrave circulation deau renouveleacutee

bull Modegravele autonome - chargement frontal utilisable en tant qursquoappareil sous-encastrable sans couvercle

bull Livreacute sans panier

bull Habillage exteacuterieur blanc ou inox

bull Equipement - Cuve entiegraverement en acier inoxydable- Pompe de circulation Qmax drsquoune capaciteacute

de 400 lmn- Pompe de vidange- Adoucisseur drsquoeau incorporeacute pour eaux froide et

chaude jusqursquoagrave 70 deg- LED pour affichage de la Tdeg temps restant et

deacuterangements- Machine agrave double paroi isoleacutee preacutevue pour

recyclage- DK condensateur agrave vapeur

bull Equipement de dosage de produits - Boicircte agrave produits en poudre dans la contreporte- Doseur de produit de rinccedilage liquide dans la

contreporte- Doseur pour produit acide DOS 1030- Preacuteeacutequipement pour module DOS C 60 pour

deacutetergents liquides avec reacuteservoir exteacuterieur

wwwanalisbeMiELE

C o N C o U R S M i E L E

Qui en Belgique a le plus vieux lave-vaisselle pour labo de MiELE

Envoyez-nous une photo de votre vieux lave-vaisselle de labo MiELE agrave lrsquoadresse e-mail cszanalisbe avec vos coordonneacutees ainsi que la mention CONCOURS MIELE

Vous pourrez gagner un superbe expresso Miele CM5100 pour votre laboratoire

Action valable jusquau 15 mars 2014

Industry 9754

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 55: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

wwwanalisbeMiELE

C o N C o U R S M i E L E

Depuis 1968 le ndeg1 des technologies de filtrationpour la protection du personnel de laboratoire

Armoires ventileacuteesagrave filtration

HottesPCR

Enceintesagrave air propre

Systegraveme de filtrationautonome pour

armoires de seacutecuriteacute

Filtres deremplacement

de toutes marques

Hottes agrave filtration sorbonnes agrave recirculation et postes de peseacutees seacutecuriseacutes

P Performances de seacutecuriteacute garanties par la norme AFNOR NF X 15-211P Aucun raccordement aeacuteraulique neacutecessaireP Des eacuteconomies drsquoeacutenergie significatives

Votre protection par le speacutecialiste de la filtrationP Technologie de filtration exclusive FlexP Pas de rejet de polluants dans lrsquoatmosphegravereP Pas de planification hottes immeacutediatement

opeacuterationnellesP Mobiles et relocalisables

wwwanalisbeERLAB

Captairreg Flexreg XLS 392

Info Olivier Lambert I Teacutel 081 25 50 65 I flashanalisbe I wwwanalisbe

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe

Page 56: Acquisition d'ARDESTA · 2018-01-11 · à droite sur la home page Analis stand Hall 2 D2 Venez découvrir nos nouvelles technologies & rencontrer nos spécialistes. Vu le succès

Nouvelle gamme de microscopes pour des applications industrielles Analyse de surface de fissures inspections de piegraveces de rechange piegraveces automobilesaeacuteronautiquesoptoeacutelectroniques analyse de mateacuteriaux composites teacuteleacutecommunication amp eacutelectroniques transformation de meacutetaux hellip

MiCRoSCoPES DRoitS Une imagerie de haut niveau et des fonctionnaliteacutes ineacutegaleacutees avec une rapiditeacute et simpliciteacute drsquoobservation quelle que soit lrsquoapplication

MiCRoSCoPES iNVERSEacuteS Reconnus pour leur qualiteacute optique leur flexibiliteacute et leur polyvalence

StEacuteREacuteoMiCRoSCoPESLes steacutereacuteomicroscopes NIKON of-frent la plus grande plage de zoom et des optiques agrave ultra-hautes per-formances pour couvrir une large gamme drsquoapplications

MiCRoSCoPE PoRtABLE NuMEacuteRiQuE puissant et compact il relegraveve un veacuteritable deacutefi pour inspecter sur site ou agrave distance de grands eacutechantillons tout en offrant des images de haute reacutesolution

Contacteznosspeacutecialistespourplusdrsquoinformationmetrologyanalisbe

wwwanalisbeNiKoN

wwwanalisbeInfo Yvan Deckers I Teacutel 09 243 77 10 I flashanalisbe