Quantification des structures 3D à partir de coupes histologiques

sériées

R. Kraftsik.

Programmes disponibles :Neurolucida - PC

structures arborescentes, coupes sériée

Volume - SGI Indigo + G.T.coupes sériées

Neurolucida

• Neuron Tracing: Trace and analyze branching neuron processes.

• Brain Mapping: Map, count cells, and measure their locations and morphology

• Morphometry: Make accurate anatomical maps and measurements of histologically distinct structures and regions

Neurolucida system:

-Neurolucida Software -Image Overlay Device

(Optical and/or Video based) -Microscope Stage Control Device -Tracing Device -Graphics Output Device -PC Computer System.

Neurolucida system, room 127

- color camera 640 X 480

- stepping stage + controller

- Zeiss microscope, light and fluorescence

Contact:

R. Kraftsik

FEATURES: - Accurately overlays a computer

graphics or text display onto the microscope image

- High brightness intensity - Up to 1024x668 pixels resolution (PC

and MAC) - Accessible controls for contrast and

brightness - Does not interfere with conventional

use of the microscope

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DRG - CR

Sciatic nerve

infra-granular cells supra-granular cells

Adult ferret

Volume Program, SGI

Contact: R. Kraftsik

Volume program, SGI. Contact R. Kraftsik

Reconstruction of cortical neurons from EM sections

Volume program, SGI. Contact R. Kraftsik

Reconstruction of dendritic segments from EM sections