DOSSIER DE CANDIDATURE A UN CONTRAT DOCTORAL EN CO …

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Appel à projets dans le cadre de la politique scientifique de l’établissement

DOSSIER DE CANDIDATURE A UN CONTRAT DOCTORAL

EN CO-TUTELLE INTERNATIONALE - 2019

Liste des pièces à joindre :

- Le présent dossier de candidature, renseigné et signé.

- Une lettre d’engagement de la structure partenaire et du co-directeur de thèse.

Si le candidat est identifié au moment du dépôt :

- un curriculum-vitae détaillé du candidat. En cas de rupture dans le cursus universitaire, donner une

explication ;

- un courrier du candidat explicitant ses motivations à poursuivre en thèse de doctorat.

Si le candidat n’a pas été identifié au moment du dépôt, le CV et le courrier du candidat ainsi que tous les

éléments d’appréciation seront à transmettre dans les meilleurs délais dès qu’il sera connu de l’unité (au plus

tard le 3 juin 2019).

Unité de recherche : E.A. 4651 Aliments Bioprocédés Toxicologie Environnements (ABTE)

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PRESENTATION DU PROJET DE THESE

Intitulé du projet de thèse : Compréhension des mécanismes d’action d’un complexe de monoglycérides et

acides gras inhibiteurs de bactéries potentiellement pathogènes et application du complexe ou de variantes

multi-composés dans des films actifs.

Le projet s’inscrit dans le cadre des accords de coopération mis en place par le carré international

Préciser lequel :

Un accord-cadre entre l’Université de Reggio et Emilia (Unimore) et l’Université de Caen Normandie a été établi

en 2016, à l’occasion de la mise en place de la première édition de l’université d’été des apprentis en agro-

alimentaire de l’ESIX Normandie (accueil des apprentis par Unimore). Cet accord est valable 5 ans et sera

complété par de nouvelles actions dont une demande d’accueil de chercheur-invité pour le Prof. Andrea

Pulvirenti sur l’année universitaire 2019-2010.

Le projet est développé dans l’un des axes transversaux de l’établissement :

Transition énergétique ;

Hadronthérapie ;

Le numérique et ses applications;

Mémoire.

Ecole doctorale : ED 497 NBISE

Unité de recherche : E .A. 4651 ABTE

Nom du directeur de thèse : Nathalie DESMASURES (co-encadrement 50%)

Unité de recherche partenaire : Department of Life Sciences, University of Modena and Reggio Emilia

Nom du co-directeur de thèse : Andrea PULVIRENTI

Nom du candidat : en cours de sélection

DESCRIPTION DU PROJET DE THESE

Description du projet (2 pages maximum) Objectifs, contexte, perspectives et précisions sur les relations

scientifiques avec la structure partenaire de la co-tutelle. (Possibilité de rédiger le projet en anglais)

Context: Microorganisms, in particular of food origin and having a Qualified Presumption of Safety (QPS) status, are an important reservoir of ingredients either as ferments or as producers of compounds having various activity within food matrices. The ability of these microorganisms to produce volatile microbial compounds (CMVs) has been known for decades, but their biological functions and antimicrobial activities have received little attention until recently. Various studies revealed that microbial volatiles can act as infochemicals in long-distance crosskingdom communication as well as antimicrobials in competition and predation (Avalos et al, 2018), so that they play an important ecological role in the regulation of species interactions within a microbiota. Some CMVs are capable of modulating growth, virulence and biofilm formation, production of specialized metabolites (eg toxins), antibiotic resistance and spore germination in competing microorganisms. CMVs belong to various classes of

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chemical compounds, including alkanes, alkenes, alcohols, esters, ketones, terpenoids, sulfur-containing compounds and inorganic compounds, and their diversity is such that there are still many CMVs to discover (O’Brien and Wright, 2011). CMVs can be unique to a single phylogenetic group or even a single species (Tyc et al, 2017), allowing their use for the selective detection of pathogens in indoor and outdoor environments and making them excellent indicators of the quality of food. Among these new compounds, some could also be used to fight against human and animal pathogens. Bacterial resistance to antibiotics is a natural phenomenon, but the spreading of multidrug resistance has been

worsened by their abuse and misuse in medicine and in farming. The biggest danger is that current antibiotics

are not able to eradicate the resistant strains. In recent years, alternative antibacterial substances have been

sought, which can help in these cases. Fatty acids and monoglycerides are known among the natural substances

for their antimicrobial properties and, important detail, bacteria do not develop resistance to them. In a previous

work at Unimore, a blend of fatty acids and their monoglycerides was created and its antimicrobial activity was

evaluated against 37 strains of Escherichia coli and 17 enterococci presenting resistance to at least two antibiotics

(Anacarso et al, 2018). Promising results were obtained with these alternative substances thus opening possible

applications to food-related Gram-positive and Gram-negative bacterial pathogens, but the mechanisms

involved in inhibition have to be elucidated.

The objectives of the project are to understand the mechanisms of action determining antimicrobial activities of

these volatile molecules and to propose and test applications relevant for the agri-food sector, and in particular

dairy and cider sectors in a transversal approach, from the cows’ udder and apples to dairy and cider

manufacturing environments. To do this:

- Starting from the UCMA microbial collection (Unicaen) the antimicrobial spectrum of the blend from

Unimore or its optimized versions will be determined by using a large collection of target microbial

species and strains relevant for the dairy and cider sectors, including Salmonella sp, Escherichia coli,

Listeria monocytogenes and Staphylococcus aureus, Zymomonas sp, Dekkera sp,

- Physiological, biochemical and molecular responses (including transcriptomic, proteomic and

metabolomics responses), cell morphology and membrane organization of representative strains will be

studied in order to describe mechanisms involved in the inhibitory activity,

- Finally, potent applications through the introduction of the mix or its optimized versions within food-

grade films will be explored in udder and stainless steel models.

In the event of satisfactory results, the perspectives of this project will be the development of antimicrobial films

to be evaluated as protective agents for teat skin in dairy cows and for apple surface, and the development of

spray to be tested for sanitation of farming/industrial equipment. Considering the components of the blend as

indicators of inhibitory activity, a screening methodology could also be developed in order to test the UCMA

microbial collection and identify potential protective strains. We want to furtherly broaden the use of natural

coatings and bioactive molecules in all the possible fields of agri-food sector.

Different formulations with bioactive products are currently studied to promote animal health and plant defense,

to reduce the abuse of antibiotics and the use of pesticides. Effective molecules have already been created during

natural evolution, we just need to find them and apply them to our human needs The present mix or its optimized

versions could be tested for their clinical application as topical treatments or, after an evaluation about the

gastric protection, as food supplements.

Scientific relations with the partner structure: this co-supervision would strengthen relations between the two

partner structures, already initiated through:

- Offers of internships at Unimore for students from Unicaen (5 students hosted in the last six years),

- Oganization of a summer university at Unimore (in 2016 and 2018) destined to students from Unicaen

(ESIX),

- Seminars conducted by Prof. A Pulvirenti at ESIX Normandie (M2 level) in 2015, 2018 and 2019,

- A request for funding to invite Prof. A Pulvirenti within Unicaen will be submitted in May 2019.

This could help to enlarge the present agreement signed by Unimore and Unicaen.

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APPRECIATION DU PROJET ET DU CANDIDAT

Le candidat a-t-il été identifié au moment du dépôt du dossier ?

Oui Non (sélection en cours parmi plusieurs candidatures spontanées)

Avis motivé du directeur de thèse sur la recherche envisagée en ce qui concerne les objectifs scientifiques et technologiques poursuivis et leur place dans les orientations de recherche du laboratoire d'accueil : Les recherches menées au sein de l’équipe MALIM de l’E.A. 4651 ABTE visent à mieux connaitre le microbiote

des aliments afin d’accompagner la production d’aliments de qualité durable. Les interactions des

microorganismes entre eux, et avec leur matrice alimentaire, sont explorées pour définir des marqueurs de

qualité et/ou d’authenticité des aliments. L’équipe MALIM s’intéresse notamment à la production par les micro-

organismes de composés à activité antimicrobienne pour le contrôle des flores d’altération et/ou pathogènes au

sein des matrices d’intérêt alimentaires. Les objectifs scientifiques et technologiques de la thèse trouvent tout à

fait leur place dans les orientations de recherche du laboratoire.

Avis du directeur d’unité sous l’angle des thématiques de recherche du laboratoire et des conditions d’accueil

du candidat :

Le projet s’intègre dans l’axe de caractérisation des propriétés probiotiques des microorganismes d’intérêt

alimentaire de l’ER MALIM du projet scientifique de l’UR ABTE tel qu’évalué par l’HCERES. Il s’appuie sur le réseau

déjà constitué par le laboratoire en région avec les acteurs socio-économique du domaine agroalimentaire. Cette

thèse en co-tutelle constituera un levier de développement stratégique pour l’UR par le renforcement de sa

visibilité internationale.

Mon avis est très favorable.

Avis motivé du directeur de l’Ecole doctorale (qualité de l’encadrement et politique doctorale du laboratoire) : En co-direction avec une équipe dynamique (Pr Andrea Pulvirenti) d’Unimore (qui bénéfice d’un accord-cadre

avec Unicaen), le Pr. Desmasures propose un sujet de thèse de microbiologie, dont l’intérêt alimentaire est ancré

dans le territoire normand. Les 2 directeurs proposés ont une expérience significative et réussie de la direction

de thèse en France et en Italie. Le Pr A Pulvirenti est à cet égard directeur de l’école doctorale des Sciences agro-

alimentaires, technologies et biotechnologies d’Unimore. L’UR ABTE est très bien intégrée à l’ED nBISE et les

docteurs qui y ont été formés ont bénéficié d’un très bon encadrement. Avis très favorable.

Date : 15 mai 2019

Signature du directeur Signature du directeur Signature du directeur

de l’Ecole doctorale de thèse de l’unité de recherche

Dipartimento di Scienze della Vita

Sede di Modena Via G. Campi 287 41125 – Modena – Italia T +39 059 2057144 – 2057140 - 2055418 F +39 059 2057146

Sede di Reggio Emilia Via G. Amendola 2 (Padiglione Besta) 42122 – Reggio Emilia – Italia T +39 0522 522051 - 522054 F +39 0522 522027

E mail [email protected] www.dsv.unimore.it Partita IVA e codice fiscale: 00427620364

Date, 16-05-2019

To whom it may concern:

with this letter of agreement, Prof. Daniela Quaglino - Director of the Department of Life Science,

University of Modena and Reggio Emilia - and Prof. Andrea Pulvirenti - Professor in food microbiology

of the Department of Life Science, University of Modena and Reggio Emilia – declare they are available

to be partner of the project: “DOSSIER DE CANDIDATURE A UN CONTRAT DOCTORAL”.

The Doctoral project is presented by Prof. Nathalie Desmasures – Professor at the University of Caen,

Normadie, France – tutor of the Doctoral project, at the Doctoral School: ED-497 NBISE, Research Unit

E.A. 4651 ABTE.

Between the University of Caen and Unimore there is a five years agreement. Prof. Pulvirenti has been

collaborating since 2007 and he is invited Professor at the Caen University from 2015.

The goal of the research is the comprehension of the mechanism of action of complex monoglycerides

and inhibitors of fatty acids in active films.

The Department of Life Science, University of Modena and Reggio Emilia, is also available to host the

Doctoral Candidate for future periods of study.

Sincerely Yours,

Director of the Department of Life Science Co-supervisor of the Doctoral Project

University of Modena and Reggio Emilia

Prof.ssa Daniela Quaglino Prof. Andrea Pulvirenti

Andrea Pulvirenti, born in 1972 in Catania.

Place of employment: Department of Life Sciences, University of Modena and Reggio Emilia, via Amendola, 2– 42124 Reggio Emilia, Italy. University position held: Associate Professor. University lectures given: Food Microbiology. Degree: food Sciences and Technologies. University of Catania, Italy, July 1996 Postgraduate studies: PhD student, University of Catania, Italy. 1997-2000 Scientific degree: PhD in Food Biotechnology, 2001 Workplaces and positions: 2000 November: Research fellow, Department of Agriculture and food Sciences, University of Modena and Reggio Emilia, (permanent position) 2006 December: Associate Professor in Food Microbiology. Department of Agriculture and food Sciences (now Dept . of Life Sciences), University of Modena and Reggio Emilia, (permanent position) From 2010 at 2017: Dean of Ph.D. School in Food Sciences, Technology and Biotechnology From November 2018: President of Master in food Technology, University of Modena and Reggio Emilia ( http://www.dsv.unimore.it/site/home/didattica/corsi-di-laurea-magistrale/articolo1003020810.html ) Publications : 134 research papers on international refereed journals Article in journal: 77 Contribution in volume (or Chapter Wise): 7 Contribution in Conference proceedings: 21 Abstracts in proceedings: 21 Monograph or scientific paper: 5 Translation of the book: 1 Curatorship: 2. Regularly invited to be a peer-reviewer for different international journals. Teaching and Tutoring Activities Since 2002 “Food Microbiology” (8 credits) and Microbiological techniques and food management (8 credits) at the University of Modena e Reggio Emilia, for food sciences students. He was a tutor of a more than 100 Master Degree student and supervisor of a 9 PhD students. Selection of scientific projects: 2004-2005 : Organizing committee of “International Symposium on Vinegars and Acetic Acid Bacteria” (www.vinegars2005.com), Reggio Emilia, Italy. 2007-2009: Coordinator of PRIN Projet “Study of the segregation of phenotypic traits for genetic improvement of strains of wine yeast strains of interest” Research Positions outside the University of Modena and Reggio Emilia: May-August 1997: Centre of Institute of Genetics - University of Florence (Italy). Field: Chromosome studies of eucaryotic microorganisms. Referee: Prof. M. Polsinelli February 1999 - February 2000 : Centre INA-INRA (Institute National of Agronomic Studies, Jouy en Josas) Laboratory of molecular genetic of microorganisms. Field: CHEF (counter-clamped homogenous electric field), PCR/RFLP, Sequencing eucariotic microorganisms. Referees: Prof. Claude Gaillardin, Dr Vang Nguyen. Lines of research: Phenotypic and genotypic characterization of microbial species of food interest. Development of molecular tools for the species/strains identification of food-related yeasts and bacteria.

http://www.dsv.unimore.it/site/home/didattica/corsi-di-laurea-magistrale/articolo1003020810.html

http://www.dsv.unimore.it/site/home/didattica/corsi-di-laurea-magistrale/articolo1003020810.html

Genotypic diversity and technological properties of yeasts species and evaluation of their potential applications in the food industry. Early detection of microbial evolution by Electronic Nose Improvement of food shelf-life by different antimicrobial methods Use of antibiotics alternative. Relevant international publications: http://personale.unimore.it/rubrica/pubblicazioni/apulvirenti

http://personale.unimore.it/rubrica/pubblicazioni/apulvirenti

Liste des publications de Pr Andrea Pulvirenti

1. Haghighi, Hossein; De Leo, Riccardo; Bedin, Elisa; Pfeifer, Frank; Siesler, Heinz Wilhelm; Pulvirenti, Andrea

Comparative analysis of blend and bilayer films based on chitosan and gelatin enriched with LAE (lauroyl arginate ethyl) with antimicrobial activity for food packaging applications

FOOD PACKAGING AND SHELF LIFE 2019

2. DE LEO, Riccardo; Quartieri, Andrea; Haghighi, Hossein; Gigliano, Silvia; Bedin, Elisa; Pulvirenti, Andrea

Application of pectin-alginate and pectin-alginate-laurolyl arginate ethyl coatings to eliminate Salmonella enteritidis cross contamination in egg shells

JOURNAL OF FOOD SAFETY 2018

3. Bedin, Elisa; Torricelli, Chiara; Gigliano, Silvia; De Leo, Riccardo; Pulvirenti, Andrea

Vegan foods: Mimic meat products in the Italian market

INTERNATIONAL JOURNAL OF GASTRONOMY AND FOOD SCIENCE 2018

4. Cammilleri, Gaetano; Costa, Antonella; Graci, Stefania; Buscemi, Maria Drussilla; Collura, Rosaria; Vella, Antonio; Pulvirenti, Andrea; Cicero, Antonello; Giangrosso, Giuseppe; Schembri, Pietro; Ferrantelli, Vincenzo

PRESENCE OF ANISAKIS PEGREFFII IN FARMED SEA BASS ( DICENTRARCHUS LABRAX L.) COMMERCIALIZED IN SOUTHERN ITALY: A FIRST REPORT

VETERINARY PARASITOLOGY 2018

5. Cammilleri, Gaetano; Graci, Stefania; Collura, Rosaria; Buscemi, Maria Drussilla; Vella, Antonio; Macaluso, Andrea; Giaccone, Vita; Giangrosso, Giuseppe; Cicero, Antonello; Lo Dico, Gianluigi Maria; Pulvirenti, Andrea; Cicero, Nicola; Ferrantelli, Vincenzo

Aflatoxin M1 in cow, sheep, and donkey milk produced in Sicily, Southern Italy

MYCOTOXIN RESEARCH 2018

6. Bellelli, Massimo; Licciardello, Fabio; Pulvirenti, Andrea; Fava, Patrizia

Properties of poly(vinyl alcohol) films as determined by thermal curing and addition of polyfunctional organic acids

FOOD PACKAGING AND SHELF LIFE 2018

7. Nunez Carmona, Estefania; Sberveglieri, Veronica; Ponzoni, Andrea; Galstyan, Vardan; Zappa, Dario; Pulvirenti, Andrea; Comini, Elisabetta

Detection of food and skin pathogen microbiota by means of an electronic nose based on metal oxide chemiresistors

SENSORS AND ACTUATORS. B, CHEMICAL 2017

8. Pulvirenti, Andrea; De Giorgio, Roberto

Le certificazioni di qualità nel settore Agroalimentare: stato dell'arte ed evoluzione book 2017

9. Sberveglieri, Veronica; Bhandari, Manohar P.; Pulvirenti, Andrea; Carmona, Estefania NÃºÃ±ez

Grated parmigiano reggiano cheese: Authenticity determination and characterization by a novel nanowire device (S3) and GC-MS

Smart Sensors, Measurement and Instrumentation 2017

10. Immacolata, Anacarso; Andrea, Quartieri; Riccardo De, Leo; Pulvirenti, Andrea

Evaluation of the antimicrobial activity of a blend of monoglycerides against Escherichia coli and Enterococci with multiple drug resistance

FOOD ANALYTICAL METHODS 2018

11. Ronga, Domenico; Setti, Leonardo; Salvarani, Chiara; De Leo, Riccardo;

Effects of solid and liquid digestate for hydroponic baby leaf lettuce (Lactuca sativa L.) cultivation

JOURNAL OF CEREAL SCIENCE 2018

Bedin, Elisa; Pulvirenti, Andrea; Milc, Justyna; Pecchioni, Nicola; Francia, Enrico

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ARCHIVES OF MICROBIOLOGY

13. Giaccone, Vita; Cammilleri, Gaetano; Macaluso, Andrea; Cicero, Nicola; Pulvirenti, Andrea; Vella, Antonio; Ferrantelli, Vincenzo

A LC-HRMS After QuEChERS Cleanup Method for the Rapid Determination of Dye Residues in Fish Products

SCIENTIA HORTICULTURAE 2018

14. Vita, Giaccone; Gaetano, Cammilleri; Vita Di, Stefano; Rosa, Pitonzo; Antonio, Vella; Pulvirenti, Andrea; Gianluigi Maria Lo, Dico; Vincenzo, Ferrantelli; Andrea, Macaluso

First report on the presence of Alloxan in bleached flour by LC-MS/MS method

FOOD ANALYTICAL METHODS 2017

15. Pulvirenti, Andrea; Zambonelli, C.; Gullo, Maria; Giudici, Paolo

In Vivo Interspecific Hybrids of Saccharomyces Species

JOURNAL OF APPLIED MICROBIOLOGY 2003

16. P. Giudici; C. Caggia; A. Pulvirenti; S. Ranieri

Karyotiping of Saccharomyces strains with different temperature profiles

LETTERS IN APPLIED MICROBIOLOGY 1998

17. Giudici P.; C. Caggia; A. Pulvirenti; C. Zambonelli; S. Rainieri

Electroforetic profiles of hybrids between cryotolerant and non-cryotolerant Saccharomyces strains

ANNALI DI MICROBIOLOGIA ED ENZIMOLOGIA 1998

18. P. Giudici; C. Caggia; A. Pulvirenti; C. Restuccia

Cryotolerant Saccharomyces strains and spoilage of refrigerated musts

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 1999

19. GIUDICI P; SOLIERI L.; PULVIRENTI A.M; CASSANELLI S

Strategies and perspectives for genetic improvement of wine yeasts. BULLETIN DE L'OIV 2005

20. PULVIRENTI A.; P. GIUDICI

Identification des levures par techniques Moléculaires

ANNALS OF MICROBIOLOGY 2003

21. Pulvirenti, Andrea; Caggia, C.; Restuccia, C.; Giudici, Paolo; Zambonelli, C.

Inheritance of mitochondrial DNA in interspecific Saccharomyces hybrids BULLETIN DE L'OIV 2000

22. Pulvirenti, Andrea; Castellari, L; DE PAOLA, M; Giudici, Paolo

Etude De La Prédominance Des Levures Sélectionnée Dans Une Fermentation De Cave

SENSORS AND ACTUATORS. B, CHEMICAL 2004

23. Gobbi, Emanuela; Falasconi, Matteo; Zambotti, Giulia; Sberveglieri, Veronica; Pulvirenti, Andrea; Sberveglieri, Giorgio

Rapid diagnosis of Enterobacteriaceae in vegetable soups by a metal oxide sensor based electronic nose

PROCEDIA ENGINEERING 2015

24. Sberveglieri, Veronica; Carmona, Estefania Núñez; Ponzoni, Andrea; Comini, Elisabetta; Galstyan, Vardan; Zappa, Dario; Pulvirenti, Andrea

Skin Microbiota Monitoring by Nanowire MOS Sensors

BIOMASS & BIOENERGY 2015

25. Pulvirenti, Andrea; Ronga, Domenico; Mannella, Lorenzo; Pecchioni, Nicola; Zaghi, Massimo; Tomasselli, Anna Rita; Mannella, Lorenzo; Pecchioni, Nicola

Pelleting is a successful method to eliminate the presence of Clostridium spp. from the digestate of biogas plants

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 2015

26. GALA E; LANDI S; SOLIERI L.; NOCETTI M; PULVIRENTI A; GIUDICI P

Diversity of lactic acid bacteria population in ripened Parmigiano Reggiano cheese

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 2008

27. Andrea, Caridi1; Rossana, Sidari; Pulvirenti, Andrea; Giuseppe, Meca; Alberto, Ritieni

Ochratoxin A adsorption phenotype: an inheritable yeast trait

2012

28. Sberveglieri, Veronica; Ongo, Emelda; Falasconi, Matteo; Concina, Isabella; Pulvirenti, Andrea; Sevilla III Fortunato,

Electronic nose and gas chromatography-mass spectrometry for the Philippine civet coffee discrimination

Sensors and Mycrosystems 2014

29. Sberveglieri, Veronica; Concina, I.; Falasconi, M.; Gobbi, E.; Pulvirenti, Andrea; Fava, Patrizia

Early detection of fungal contamination on green coffee by a MOX sensors based Electronic Nose

LECTURE NOTES IN ELECTRICAL ENGINEERING 2011

30. Sberveglieri, Veronica; Estefania Nunez Carmona, ; Pulvirenti, Andrea

Nanowire technology to Asses the bacterial presence in water and other food stuff

CANADIAN JOURNAL OF MICROBIOLOGY 2015

31.

Pulvirenti, Andrea; Solieri, Lisa; DE VERO, Luciana; Giudici, Paolo

Limitations on the use of polymerase chain reaction restriction fragment length polymorphism analysis of the rDNA NTS2 region for the taxonomic classification of the species Saccharomyces cerevisiae.

FEMS YEAST RESEARCH 2005

32. Rainieri, Sandra; Kodama, Yukiko; Nakao, Yoshihiro; Pulvirenti, Andrea; Giudici, Paolo

The inheritance of mtDNA in lager brewing strains

INTERNATIONAL JOURNAL ON SMART SENSING AND INTELLIGENT SYSTEMS 2008

33. Nunez Carmona, Estefania; Sberveglieri, Veronica; Ponzoni, A.; Zappa, D.; Pulvirenti, Andrea

Detection of microbial contamination in potable water by Nanowire technology

JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY 2014

34. Pulvirenti, Andrea; Solieri, Lisa; Sara, Landi; Giudici, Paolo The Yeasts of sourdough

INDUSTRIE ALIMENTARI 2006

35. Lanza, CARMELA M.; Filippo, Tomaselli; Pulvirenti, Andrea

Effetti del confezionamento sulla conservabilità delle paste di mandorla


36.

Vang, NGUYEN H.; Pulvirenti, Andrea; Claude, Gaillarden

Rapid differentiation of the closely related Kluyveromyces lactis var. lactis and K. marxianus strains isolated from dairy products using selective medium and PCR/RFLP of the rDNA non trascribed spacer 2


37. Lanza, CARMELA M.; Filippo, Tomaselli; Giuseppe, Muratore; Pulvirenti, Andrea

Studio sulla conservabilità delle brioche da gelato.

FEMS MICROBIOLOGY LETTERS 2000

38. Pulvirenti, Andrea; Nguyen, Hv; Caggia, C.; Giudici, Paolo; Rainieri, S.; Zambonelli, C.

Saccharomyces uvarum, a proper species within Saccharomyces sensu stricto


39. Caggia, C.; Restuccia, C.; Pulvirenti, Andrea; Giudici, Paolo

Identification of Pichia anomala isolated from yoghurt by RFLP of the ITS region

FEMS MICROBIOLOGY LETTERS 2001

40. Rainieri, S.; Zambonelli, C.; JOHON HALLSWORT, E.; Pulvirenti, Andrea; Giudici, Paolo

Saccharomyces uvarum, a distinct group within Saccharomyces sensu stricto


41. Caridi, A.; Pulvirenti, Andrea; Restuccia, C.; Sidari, R.

Screening for yeasts able to hydrolyse arbutin in the presence of glucose or ethanol


42. Pulvirenti, Andrea; Zambonelli, C.; Todaro, Mary Antonio Donatello; Giudici, Paolo

Interspecific hybridisation by digestive tract of invertebrates as a source of environmental biodiversity within the Saccharomyces cerevisiae


43. Restuccia, C.; Pulvirenti, Andrea; Caggia, C.; Giudici, Paolo

A b-glucosidase positive strain of Saccharomyces cerevisiae isolated from grape must



Inerspecific hybridisation by digestive tract of invertebrates as a source of enviromental biodiversity within the Saccaromyces cerevisiae species



Origin of Biodiversity of Saccharomyces cerevisiae and its Interspecific hybrids.


46. Gullo M; Romano AD; Pulvirenti A; Giudici P

Candida humilis - dominant species in sourdoughs for the production of durum wheat bran flour bread

LETTERS IN APPLIED MICROBIOLOGY 2003

47. A. Pulvirenti; L. Solieri; M. Gullo; L. De Vero; P. Giudici

Occurrence and dominance of yeast species in sourdough


48. De Vero L.; A. Pulvirenti; M. Gullo; P. Bonatti; P. Giudici

Sorting of mitocondrial DNA and proteins in the progeny of Saccharomyces interspecific hybrids


49. A. Pulvirenti; C. Caggia; C. Restuccia; M. Gullo; P. Giudici

DNA fingerprinting methods used for identification of yeasts isolated from Sicilian sourdoughs


50. Zambotti, G.; Sberveglieri, V.; Gobbi, E.; Falasconi, M.; Nunez carmona, Estefania; Pulvirenti, A.

Fast Identification of Microbiological Contamination in Vegetable Soup by Electronic Nose


51. Núñez Carmona, Estefania; Sberveglieri, Veronica; Comini, Elisabetta; Zappa, Dario; Pulvirenti, Andrea

Nanowire Technology for the Detection of Microorganisms in Potable Water


52. Sberveglieri, V.; Núñez carmona, E.; Zappa, D.; Comini, E.; Pulvirenti, A.

Classification of Different Roasting Processes by MOX Nanowire


53. V. Sberveglieri; Matteo Falasconi; Emanuela Gobbi; E. Núñez Carmona; Giulia Zambotti; A. Pulvirenti

Candida Milleri Detected by Electronic Nose in Tomato Sauce

PROCEEDINGS OF THE ... INTERNATIONAL CONFERENCE ON SENSING TECHNOLOGY 2014

54. Sberveglieri, Veronica; Carmona, Estefania Nunez; Pulvirenti, Andrea

Nanowire device (S3) to characterize the genuine aroma of Parmigiano Reggiano cheese

Proceedings of the International Conference on Sensing Technology, ICST Volume 2016 2016

55. Sberveglieri, V; Carmona, E Nunez; Pulvirenti, A

Nanowire sensor array for the rapid quality control on grated Parmigiano Reggiano

Report of AISEM Annual Conference, 2015 XVIII 2015

56. Riccardo, de leo; Francesca, Diazzi; Andrea, Quartieri; Andrea, Pulvirenti

Elimination of Salmonella cross-contamination on eggs using antimicrobial coating


57. L. SOLIERI; L. DE VERO; M. GULLO; A. PULVIRENTI

Studio molecolare e fenotipico di lieviti in paste acide tradizionali


58. Chiari, Paola; Pulvirenti, Andrea; Giudici, Paolo

Dessert freddi: criteri per la loro classificazione [Cold dessert: criteria for their classification]


59. V.Sberveglieri; A. Pulvirenti; P. Giudici

Dalla valutazione qualitativa ai modelli di previsione dei rischi ignoti book 2014

60.

A. PULVIRENTI; L. SOLIERI; L. DE VERO; M. GULLO; P. GIUDICI

Limits of r-DNA-NTS2 regions on the taxonomy of Saccharomyces genus

Report of 11th International Congress on Yeasts. Yeasts in Sciences and Biotechnology 2004

61.

A.Pulvirenti; S.Boveri Shelf-life improvement of horse meat by preservative lactic cultures


62. Carmona, Estefania Nunez; Sberveglieri, Veronica; Ponzoni, Andrea; Zappa, Dario; Pulvirenti, Andrea

Small Sensor Sistem S3 device to control the microbial contamination in water

PROCEEDINGS OF THE INTERNATIONAL CONFERENCE ON SENSING TECHNOLOGY 2016

63.

Pulvirenti, Andrea Raw meat shel life increasing by antimicrobial edible coating


64. Sberveglieri, Veronica; Comini, Elisabetta; Zappa, Dario; Pulvirenti, Andrea; Carmona, Estefania Nunez

Electronic nose for the early detection of different types of indigenous mold contamination in green coffee


65. Carmona, Estefania Nunez; Sberveglieri, Veronica; Pulvirenti, Andrea

Detection of microorganisms in water and different food matrix by Electronic Nose 2013

66. V. Sberveglieri; I. Concina; M. Falasconi; E. Ongo; A. Pulvirenti; P. Fava

Identification Of Geographical Origin Of Coffee Before And After Roasting By Electronic Noses

SUBSURFACE SENSING TECHNOLOGY AND APPLICATIONS 2011

67. Sberveglieri, V.; Fava, P.; Pulvirenti, A.; Concina, I.; Falasconi, M.

New methods for the early detection of fungal contamination on green coffee beans by an Electronic Nose

JOURNAL OF FOOD SCIENCE 2012

68. Musetti, Alessandro; Paderni, Katia; Fabbri, Paola; Pulvirenti, Andrea; Al-Moghazy, Marwa; Fava, Patrizia

Poly(vinyl alcohol)-Based Film Potentially Suitable for Antimicrobial Packaging Applications.


69.

S. Boveri; S. Rainieri; A. Pulvirenti

Method for the validation of intraspecific crosses of Saccharomycescerevisiae strains by minisatellite analysis

BIOMED RESEARCH INTERNATIONAL 2012

70. V. Sberveglieri;E. Nunez Carmona;Elisabetta Comini;Andrea Ponzoni;Dario Zappa;Onofrio Pirrotta;A. Pulvirenti

A Novel Electronic Nose as Adaptable Device to Judge Microbiological Quality and Safety in Foodstuff FOOD CONTROL 2014

71. Al-Moghazy; M; Boveri; S; Pulvirenti, A

Microbiological safety in pistachios and pistachio containing products


72. Gian Luigi Mazzacan; Andrea Pulvirenti; Fausto De Salvo; Paola Zucchi

Il processo fermentativo in mieli di sulla ed erba medica:valutazione della specie di lieviti dominante SENSOR LETTERS 2011

73. Sberveglieri, V.; Carmona, E. Nunez; Falasconi, M.; Concina, I.; Pulvirenti, A.

Fast Identification of Different Kind of Green Coffee by Electronic Nose

PROCEEDINGS OF THE INTERNATIONAL CONFERENCE ON 2014

SENSING TECHNOLOGY

74. Veronica Sberveglieri; Isabella Concina; Matteo Falasconi; Andrea Pulvirenti; Patrizia Fava

Discrimination between different type of coffee according to their country of origin TRANSPLANTATION 2012

75.

Pulvirenti, A; Solieri, L; DE VERO, L; Giudici, P

Mitochondrial DNA and Mitochondrial proteins in interspecific Saccharomyces hybrids


76. V. Sberveglieri; A. Pulvirenti; E. Comini; E. Nunez Carmona

What happens at the aroma of coffee beans after roasting?


77. PAOLO GIUDICI; MARIA GULLO; LISA SOLIERI; LUCIANA DE VERO; SARA LANDI; A. PULVIRENTI; SANDRA RAINIERI

Le Fermentazioni dell’Aceto Balsamico Tradizionale book 2006

78.

Pulvirenti A.; C. Caggia; S. Rainieri; P. Giudici

Pulsed field gel electrophoresis: optimisation of the running conditions to improve the differentiation of Saccharomyces cerevisiae and Saccharomyces bayanus strains

ISSY congress. Yeasts in the spoilage production and spoilage of food and beverages 1998

79. DANIELA MIGLIORISI; CINZIA CAGGIA; A. PULVIRENTI; PAOLO GIUDICI I batteri lattici del pecorino siciliano IL LATTE 1997

80. Andrea Pulvirenti; Sandra Rainieri; Silvio Boveri; Paolo Giudici

Strategy for optimizing the selection of yeast starter cultures by pre-selecting strains with good technical characteristics


81. PAOLO GIUDICI; MARIA CONCETTA STRANO; A. PULVIRENTI

La sicurezza microbiologica nella preparazione casalinga delle olive verdi al naturale TECNICA AGRICOLA 1997

82. Solieri L.; Pulvirenti A.; Gullo M.; Giudici P.

Dominant yeast species in traditional sourdoughs

proceeding

2003

83. L. SOLIERI; L. CASTELLARI; A. R. BATTAGLIOLA; A. PULVIRENTI; S. LANDI; P. GIUDICI

Molecular procedure for yeasts identification of Traditional Balsamic Vinegar

proceeding

2005

84. L. Solieri; A. Pulvirenti; A.R. Battagliola; M. Gullo; L. De Vero; P. Giudici

I lieviti dell’Aceto Balsamico Tradizionale dopo tre lustri.

proceeding

2004

85.

P. Giudici; M. Gullo; A. Pulvirenti; L. De Vero; L. Solieri;

I microrganismi dell'aceto balsamico. Atti convegno:"anche la tradizione va studiata" ricerche preliminari per l'individuazione di starter per l'Aceto Balsamico Tradizionale.

proceeding

2004

86. LUCIANA DE VERO; ANDREA PULVIRENTI; MARIA GULLO; LISA SOLIERI; P. GIUDICI

Transmission of mtdna and mitochondrial proteins in the progeny of Saccharomyces interspecific hybrids.

proceeding

2003

87. P. GIUDICI; MARIA GULLO; ANDREA PULVIRENTI; LISA SOLIERI; LUCIANA DE VERO.

Microflora Y La Tecnología De Producción Del Vinagre Balsámico Tradicional.

proceeding

2003

88. M. Gullo; A. Pulvirenti; L. De Vero; P. Giudici

I batteri acetici dell’Aceto Balsamico Tradizionale: caratterizzazione fenotipica e molecolare.

proceeding

2004

89. A. Pulvirenti Microbiologia degli Alimenti Book springer 2009

90. L. SOLIERI; L. DE VERO; A. PULVIRENTI; M. GULLO; P. GIUDICI; S. CASSANELLI

Analysis of genes and proteins targeted to yeast mitochondria in Saccharomyces interspecific hybrids.

proceeding

2004

91.

V.Sberveglieri; A.Pulvirenti; P.Fava

THE DEVELOPMENT OF A NEW METHOD FOR RAPID IDENTIFICATION OF FUNGAL CONTAMINATION ON GREEN COFFEE BY MOX SENSORS BASED ELECTRONIC NOSE

proceeding

2012

92.

Marwa Al-Moghazy; Silvio Boveri; Andrea Pulvirenti

Microbiological safety and moleculaar analysis of Klebsiella ssp. and Salmonella ssp. Isolated from different pistachio's product

proceeding

2011

93. VERONICA SBERVEGLIERI; ISABELLA CONCINA; MATTEO FALASCONI; ANDREA PULVIRENTI; PATRIZIA FAVA

DISCRIMINATION BETWEEN DIFFERENT TYPES OFCOFFEE ACCORDING TO THEIR COUNTRY OF ORIGINPresentazione oraleVERONICA SBERVEGLIERI

proceeding

2011

94. Sberveglieri V.; Concina I. ; Falasconi M.; Gobbi E.; Pulvirenti A. ; Fava P.

DETERMINATION BY ELECTRONIC NOSE OF COFFEE FROM DIFFRENT GEOGRAPHICAL AREAS

proceeding

2011

95.

Sberveglieri V.; Concina I. ;Falasconi M.; Gobbi E.; Pulvirenti A. ; Fava P.

SETTIN UP A NEW METHOD FOR RAPID IDENTIFICATION OF FUNGAL CONTAMINATION ON GREEN COFFEE BY MOX SENSORS BASED ELETRONIC NOSE

proceeding

2011

96. Marwa Al-Moghazy; Andrea Pulvirenti; Patrizia Fava

Application of antimicrobial alginate-oregano essential oil edibile coating on chicken met

Food Microbiologi specia ediction 2011

97. Andrea Caridi; Rossana Sidari; Andrea Pulvirenti; Giuseppe Mecca; Alberto Ritieni.

Ochratoxin A adsorption phenotype: an inheritable yeast trait EUROCARNI 2008

98. A. PULVIRENTI; S. BOVERI; M. MASINA

Colture lattiche protettive e miglioramento della shelf-life della carne equina

INGREDIENTI ALIMENTARI 2009

99. A. Pulvirenti; P. Zucchi; F. Desalvo; G. Marcazzan; A. G. Sabatini; P. Giudici

Aspetti qualitativi del miele fermentato

proceeding

2009

100. P. Giudici; M. Gullo; L. De Vero; A. Pulvirenti; U. Rangone.

Ricerche finalizzate alla tutela e tipicità dell'Aceto Balsamico Tradizionale di Reggio Emilia

proceeding

2004

101. P. Giudici; M. Gullo; L. Solieri; L. De Vero; A. Pulvirenti; W. Ludwig; F. Barja; J. Bourgeois; J. M.Guillamon; A. Mas; P.Raspor

vinegars and acetic acid bacteria international symposium

proceeding

2005

102. Andrea Caridi; Rossana Sidari; Andrea Pulvirenti; Giuseppe Blaiotta e Alberto Ritieni

Wine strain improvement strategies to enhance red wine safety based on parietal adsorption activity

proceeding

2009

103. P.Giudici; A. Pulvirenti; M. Gullo Microbiologia degli aceti

proceeding

2002

104. P. Giudici; L.Solieri; M. Gullo; L. De Vero; A. Pulvirenti; P. Reverberi; G. Gambini

Titolo progeto: ruolo dei microrganismi del siero innesto nella produzione di formaggio parmigiano reggiano di qualita’

proceeding

2006

105. P. Giudici; A. Pulvirenti

Dominanza dei LSA sui ceppi selvaggi APOIDEA 2004

106. A. PULVIRENTI; S. BOVERI ; P. ZUCCHI; F. DESALVO; G. MARCAZZAN.

Zygosaccharomyces mellis specie dominante nei processi fermentativi in mieli di agrumi, castagno, girasole, melata. VIGNEVINI 2009

107. A. Pulvirenti; M. De Paola

LA PREVALENZA DEI LIEVITI STARTER IN ENOLOGIA

proceeding

2008

108. Giuseppe Blaiotta; Valentina Coppola; Alberto Ritieni; Andrea Pulvirenti; Andrea Caridi

Selection of Saccharomyces cerevisiae strains with high ability to adsorb ochratoxin A during fermentation

proceeding

2009

109.

A Pulvirenti

Ricerche finalizzate alla tutela della tipicità dell'aceto balsamico di Reggio Emilia

proceeding

2004

110.

Andrea Carldi Rossana Sicari. Caterina Vadalà; Andrea Pulvirenti

Wine strain improvement strategies to enhance red wine safety based on parietal adsorption activity. Selection of Spanish, Portuguese and Sicilian wine yeasts.

proceeding

2009

111. P. Giudici; L. Solieri; M.Gullo; A. Pulvirenti; L. De Vero; P.M. Falcone I microrganismi dell'aceto balsamico.

proceeding

2006

112.

Andrea PULVIRENTI

Studio della segregazione dei caratteri fenotipici per il miglioramento genetico di ceppi di ceppi di livito di interesse enologico

SCIENZA E TECNICA LATTIERO-CASEARIA 2008

113.

L. De Vero; L. Solieri; E. Gala; A. Pulvirenti; P.Giudici

Studio dell’evoluzione della popolazione di batteri lattici durante la produzione di Parmigiano Reggiano mediante 16S rDNA PCR-DGGE


114.

P. Zucchi; A. Pulvirenti; F. De Salvo; G. L. Mazzacan

Monitoring the fermentation process in honey and the evaluationof chemical, microbiological and sensory parameters

INTERNATIONAL JOURNAL OF ELECTROCHEMISTRY 2012

115. M. Falasconi; I. Concina; E. Gobbi; V. Sberveglieri; A. Pulvirenti; G. Sberveglieri

Electronic Nose for Microbiological Quality Control of Food Products

INDUSTRIE DELLE BEVANDE 2012

116. P. GIUDICI; A. PULVIRENTI; L. DE VERO; S. LANDI; M. GULLO

Cristallizzazione dell'aceto balsamico tradizionale

proceeding

2004

117. L. Solieri; L. Castellari; A.R. Battagliola; A. Pulvirenti; S. Landi; P. Giudici

The yeasts of Traditional Balsamic Vinegar

proceeding

2005

118. L. De Vero; A. Pulvirenti; M. Gullo; L. Solieri; P. Giudici

Transmission of mt DNA and mitochondrial proteins in the progeny of Saccharomyces hybrids

proceeding

2003

119. Giudici P.; Gullo M.; Solieri L.; De Vero L.; Landi S.; Pulvirenti A.; Rainieri S

Le fermentazioni dell’aceto balsamico tradizionale

proceeding

2006

120. P. GIUDICI; A. PULVIRENTI

Molecular Methods for identification of wine yeasts

proceeding

2002

121. A. PULVIRENTI; M. DE PAOLA; L. CASTELLARI; P. GIUDICI

Prevalence of the inoculated strain on the native yeast during wine fermentation


122. P. GIUDICI; A. PULVIRENTI; C. CAGGIA.

L’acido gluconico e l’acido lattico come indici di qualità microbiologica dei succhi d’arancia


123.

Pulvirenti A.; Zambonelli C.; Todaro M.A.; Giudici P.

Interspecific hybridation by digestive tract of invertebrates as sorce of environmental biodiversity within the Saccaromyces cerevisiae. proceeding 2002

124.

Pulvirenti A.; Giudici P.

Estudio del predominio de las levaduras seleccionadas en la fermentacion en bodega


125. S. RAINIERI; A. PULVIRENTI; C. CAGGIA; P. GIUDICI

Electrophoretic Profile of Thermotolerant Saccaromyces cerevisiae

proceeding

1998

126. Caggia C.; Restuccia C.; Pulvirenti A.; Giudici P.

Identification of yeasts isolated from spoiled yoghurt

proceeding

1999

127.

A. Pulvirenti; P. Giudici

Metodi molecolari per l’identificazione di lieviti isolati dalle paste acide siciliane.

proceeding

2001

128. A. Pulvirenti; M. Gullo; L. De Vero; P. Giudici

Uniparental Mitochondrial Inheritance in Saccaromyces spore coniugation.

proceeding

2002

129. A. PULVIRENTI, L. SOLIERI, M. GULLO; P. GIUDICI

Metodi indiretti per lo studio dei microrganismi dell’ABT. BULLETIN DE L'OIV 2004

130. GIUDICI P.; PULVIRENTI A.; TODARO M.A.; ZAMBONELLI C.

Horizontal transfer of genetic materials in Saccharomyces promoted by animals


131. PAOLO GIUDICI; SANDRA RAINIERI; A. PULVIRENTI; CARLO ZAMBONELLI. . TRADIZIONE E NUOVI APPROCCI

Strategie di selezione e miglioramento genetico


132. P. GIUDICI; CAGGIA C.; PULVIRENTI A.

Cryotolerant Saccharomyces strains and spoilage o f refrigerated must TECNICA AGRICOLA 2000

133. P. GIUDICI; M.C. STRANO; A.PULVIRENTI.

La sicurezza microbiologica nella preparazione casalinga delle olive verdi al naturale siciliane. TECNICA AGRICOLA 1997

134. P. Giudici; A. Pulvirenti; S. Cassanelli; L. De Vero; M. Gullo

Novel microorganisms in bread production and cereals and how to create them

proceeding

2004

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